Heat shock protein (HSP) expression and mitogen-activated protein kinase (MAPK) phosphorylation during early embryonic developmental stages of the Gilthead sea bream (Sparus aurata)

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Published: Jul 30, 2018
DOI: https://doi.org/10.12681/mms.14088
Keywords:
Sparus aurata HSP70 HSP90 p38MAPK ERKs JNKs early development
CHRYSOULA ROUFIDOU
Laboratory of Animal Physiology, Department of Zoology, Faculty of Sciences, School of Biology, Aristotle University of Thessaloniki Present address:Department of Zoology, Stockholm University, S-106 91 Stockholm, Sweden
https://orcid.org/0000-0003-0022-0698
KONSTANTINOS FEIDANTSIS
Laboratory of Animal Physiology Department of Zoology School of Biology Faculty of Science Aristotle University of Thessaloniki Thessaloniki Greece
ELENI MENTE
Department of Ichthyology and Aquatic Environment, School of Agricultural Sciences, University of Thessaly, GR-38446 Volos, Greece
ELENA SARROPOULOU
Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, 71003 Heraklion, Crete
EFTHIMIA ANTONOPOULOU
School of Biology, Aristotle University of Thessaloniki
https://orcid.org/0000-0003-4565-6962
Abstract

Both heat shock proteins (HSPs), which have key roles in vital cell functions, as well as members of the mitogen-activated protein kinases (MAPKs), which adjust gene expression by transducing cellular signals to the nucleus, are necessary for normal embryonic development in vertebrates. Therefore, protein expression levels of HSP70 and HSP90 and the activation of members of the MAPK protein family, such as p38 MAPK, ERKs, and JNKs were studied in the early developmental stages of the Gilthead sea bream, Sparus aurata Linnaeus, 1758. The protein expression of HSP70 and the phosphorylation ratio of JNKs remained at equal levels at all examined developmental stages, while the other examined proteins exhibited a differential profile. HSP90 levels were mostly increased at the 16-cell stage and towards the morula stages, and the lowest values were observed at the two- to four-cell and one-half epiboly stages. While p38 MAPK phosphorylation ratio exhibited increased values mostly in the early developmental stages, the opposite was observed concerning ERK phosphorylation ratio, where increased values were observed in the later embryonic stages (high blastula to one-half epiboly stages). These differential profiles of the examined protein expression levels highlight the importance of these proteins during embryogenesis and pave the way for further research to unveil their distinct role in early development.

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Author Biography
EFTHIMIA ANTONOPOULOU, School of Biology, Aristotle University of Thessaloniki
Laboratory of Animal Physiology
Department of Zoology
School of Biology
Faculty of Science
Aristotle University of Thessaloniki
Thessaloniki
Greece
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