Antibiotic resistance of Staphylococcus intermedius strains isolated from 53 natural cases of canine pyoderma

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Published: Jan 25, 2018
DOI: https://doi.org/10.12681/jhvms.15388
Keywords:
Staphylococcus intermedius dog pyoderma antibiotic resistance
M. N. SARIDOMICHELAKIS (Μ. Ν. ΣΑΡΙΔΟΜΙΧΕΛΑΚΗΣ)
Clinic of Companion Animal Medicine, Faculty of Veterinary Medicine, Aristo ties University of Thessaloniki
A. F. KOUTINAS (Α.Φ. ΚΟΥΤΙΝΑΣ)
Clinic of Companion Animal Medicine, Faculty of Veterinary Medicine, Aristo ties University of Thessaloniki
N. HELIADIS (Ν. ΗΛΙΑΔΗΣ)
Department of Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, Aristotles University of Thessaloniki
I. GEORGOPOULOU (Ι. ΓΕΩΡΓΟΠΟΥΛΟΥ)
Clinic of Poultry Diseases, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki
L. LEONTIDES (Λ. ΛΕΟΝΤΙΔΗΣ)
Laboratory of Biostatistics, Epidemiology and Economics of Animal Production, School of Veterinary Medicine, University of Thessaly
Abstract

Samples for bacterial cultures on blood agar were obtained from the skin lesions of 53 dogs demonstrating the typical clinical and cytological features of superficial (n=17) or deep (n=36) pyoderma. None of them had received any kind of antimicrobial therapy within the previous month. The Staphylococcus looking colonies were recultured on blood and Chapman agar for staphylococcal species identification and biotyping. All 53 coagulasepositive staphylococcal isolates were eventually identified as S. intermedius. The susceptibility for each of these isolates was tested against 12 different antibiotics with the disc diffusion method. Resistance rates were found to be 0% for amoxicillin/clavulanate, 11.3% for oxacillin, 7.5% for cefalexin, 5.7% for enrofloxacin, 32.1% for erythromycin, 11.3% for tylosin, 20.8% for lincomycin, 18.9% for clindamycin, 32.1% for doxycycline, 5.7% for amicacin, 7.5% for chloramphenicol and 92.5% for trimethoprim/sulfamethoxazole. No correlation between the resistance pattern and either the age, breed, sex, and the animals' lifestyle, or the depth of skin infection, the presence or not of underlying diseases and any past treatment with antibiotics was identified.

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