Seroprevalence of Mycoplasma bovis in grazing dairy cows from five different areas in Serbia

PDF
Published: Feb 5, 2019
DOI: https://doi.org/10.12681/jhvms.16681
Keywords:
Mycoplasma bovis ELISA serology grazing cows Serbia
Dragica Vojinovic
Scientific Veterinary Institute of Serbia
https://orcid.org/0000-0002-4890-3239
Nemanja Zdravkovic
Veterinary Scientific Institute of Serbia Belgrade
https://orcid.org/0000-0002-3925-4409
Radiša Prodanović
University of Belgrade, Faculty of Veterinary Medicine
Ivan Vujanac
University of Belgrade, Faculty of Veterinary Medicine
Svetlana Nedić
University of Belgrade, Faculty of Veterinary Medicine
Nektarios D. Giadinis
Aristotle University of Thessaloniki, Faculty of Veterinary Medicine
Nikolaos Panuonis
Aristotle University of Thessaloniki, Faculty of Veterinary Medicine
Marija Manic Manic
Veterinary Specialistic Institute Nis
Dejan Bugaraski
Scientific Veterinary Institute Novi Sad
Milija Palamarevic
University of Belgrade, Faculty of Veterinary Mednicine
Nataša Bogicevic
University of Belgrade, Faculty of Veterinary Mednicine
Ivan Dobrosavljevic
Veterinary-specialistic institute "Pozarevac"
Ljiljana Spalević
Scientific Veterinary Institute of Serbia
Jadranka Žutić
Scientific Veterinary Institute of Serbia, Department for serology.
Jasna Prodanov-Radulović
Scientific Veterinary Institute Novi Sad
Jovan Bojkovski
University of Belgrade, Faculty of Veterinary Institute
Abstract

Mycoplasma bovis infection in grazing dairy cows has not been reported in the Republic of Serbia to date. It is important to monitor its seroprevalence on the field. The presence of specific antibodies against M. bovis in the blood serum of grazing dairy cows is investigated in the present study. A total of 131 blood serum samples of clinically healthy dairy cows were examined. Sampling was performed during 2013 from five different areas in Serbia: Zasavica, Pozarevac, Gruza, Novi Sad and Banatski Karlovac. A commercial ELISA kit for diagnosis of M. bovis antibodies in blood serum samples, manufactured by Bio-X Diagnostics, Belgium, was used. Specific antibodies against M. bovis were identified in 13 out of 131 samples (9.92%) from 4 locations; the only negative location was the most southern Gruza. The revealed seroprevalence is evidence for the presence of M. bovis in grazing dairy cows in different locations of Serbia.

Article Details
  • Section
  • Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
Download data is not yet available.
Author Biographies
Dragica Vojinovic, Scientific Veterinary Institute of Serbia
Department for serology. Junior researcher.
Nemanja Zdravkovic, Veterinary Scientific Institute of Serbia Belgrade
Bacteriology diagnostic laboratory, assistent researcher
Radiša Prodanović, University of Belgrade, Faculty of Veterinary Medicine
Department of Ruminants diseases, assistant professor
Ivan Vujanac, University of Belgrade, Faculty of Veterinary Medicine
Department of Ruminants diseases, asociate proffesor
Svetlana Nedić, University of Belgrade, Faculty of Veterinary Medicine
Department of Reproduction, Junior rersearcher
Nektarios D. Giadinis, Aristotle University of Thessaloniki, Faculty of Veterinary Medicine
Clinic of Farm Animals
Nikolaos Panuonis, Aristotle University of Thessaloniki, Faculty of Veterinary Medicine
Clinic of Farm Animals, Associate Professor
Marija Manic Manic, Veterinary Specialistic Institute Nis
Diagnostic Laboratory
Dejan Bugaraski, Scientific Veterinary Institute Novi Sad
Health protection of ruminants and hoofed animals, Research associate
Milija Palamarevic, University of Belgrade, Faculty of Veterinary Mednicine
Department for Ruminants diseases, phD Student
Nataša Bogicevic, University of Belgrade, Faculty of Veterinary Mednicine
Department for Infective diseases and diseases of bees, junior researcher
Ivan Dobrosavljevic, Veterinary-specialistic institute "Pozarevac"
Patology Department, Senior stuff
Ljiljana Spalević, Scientific Veterinary Institute of Serbia
Assistent researcher
Jadranka Žutić, Scientific Veterinary Institute of Serbia, Department for serology.
Head of Department for serology, Associate researcher
Jasna Prodanov-Radulović, Scientific Veterinary Institute Novi Sad
Health protection of ruminants and hoofed animals, Research associate
Jovan Bojkovski, University of Belgrade, Faculty of Veterinary Institute

Department of Ruminans disease, Full professor

 

References
Al-Farha AA, Hemmatzadeh F, Khazandi M, Hoare A, Petrovski K (2017) Evaluation of effects of Mycoplasma mastitis on milk composition in dairy cattle from South Australia. BMC Vet Res. 13:351.
Ayling RD, Bashiruddin SE, Nicholas RAJ (2004) Mycoplasma species and related organisms izolated from ruminants in Britain between 1990 and 2000. Vet Rec 155:431-416.
Bell CJ, Blackburn P, Patterson IA, Ellison S, Ball HJ (2012) Real-time PCR demonstrates a higher prevalence of Mycoplasma bovis in Northern Ireland compared with sandwich ELISA. Vet Rec 171:402.
Blackburn P, Brooks C, McConnell W, Ball HJ (2007) Isolation of Mycoplasma bovis from cattle in Northern Ireland from 1999 to 2005. Vet Rec 161:452-453.
Burgi N, Josi C, Burki S, Schweizer M, Pilo P (2018) Mycoplasma bovis co-infection with bovine viral diarrhea virus in bovine macrophages. Vet Res 49:2.
Byrne WJ, McCormack R, Ball HJ, Brice N, Baker SE, Ayling RD, Nicholas RAJ (2000) Application of an indirect ELISA to milk samples to identify cows with Mycoplasma bovis mastitis. Vet Rec 146:368-369.
Cerdá R, Xavier J, Sansalone P, de la Sota R, Rosenbush R (2000) Isolation of Mycoplasma bovisduring an outbreak of bovine mastitis at a dairy farm in the province of Buenos Aires. 1st report in the Republic of Argentina. Rev LatinoamMicrobiol 42:7-11.
Fekete SG, Kells RO (2007) Interrelationship of feeding with immunity and parastic infection: a review. Vet Med (Czech) 52:131-143.
Filioussis G, Christodoulopoulos G, Thatcher A, Petridou V, Bourtzi-Chatzopoulou E (2007) Isolation of Mycoplasma bovis from bovine clinical mastitis cases in Northern Greece. Vet J 173:215-218.
Gagea M, Bateman K, Shanahan R, Dreumel T, Mcewen B, Carman S, Archambault M, Caswell J (2006) Naturally occuring Mycoplasma bovis-associated pneumonia and polyarthritis in feedlot beef calves. J Vet Diagn Invest 18:29-40.
Gonsales RN, Jayarao BM, Oliver SP, Sears PM (1983) Pneumonia, arthritis and mastitis in dairy cows due to Mycoplasma bovis. In: Proceedings of the 32nd Annual Meeting of the National Mastitis Council Kansas City, MO, USA 32:178-186.
Haapala V, Pohjanvirta T, Vähänikkilä N, Halkilahti J, Simonen H, Pelkonen S, Soveri T, Simojoki H, Autio T (2018)Semen as a source of Mycoplasma bovis mastitis in dairy herds. Vet Microbiol 216:60-66.
Hale HH, Helmbold CF, Plastridge WN, Stula EF (1962) Bovine mastitis by Mycoplasma species. Cornell Vet 52:582-591.
Le Grand D, Calavas D, Brank M, Citti C, Rosengarten R, Bezille P, Poumarat F (2002) Serological prevalence of Mycoplasma bovis infection in suckling beef cattle in France. Vet Rec 150:268-273.
Maksimovic Z, Rifatbegovic M (2012) Mycoplasmas isolated from the respiratory tract of cattle in Bosnia and Herzegovina. An Vet (Murcia) 28:79-83.
Nicholas RAJ, Ayling RD (2003) Mycoplasma bovis: disease, diagnosis, and control. Res Vet Sci 74:105-112.
Nicholas RAJ, Baker S, Ayling RD, Stripkovits L (2000) Mycoplasma infections in growing cattle. Cattle Pract 8:115-118.
OIE (2018) Old Classification of Diseases Notifiable to the OIE List A. http://www.oie.int/en/animal-health-in-the-world/the-world-animal-health-information-system/old-classification-of-diseases-notifiable-to-the-oie-list-a/. [accessed 20.03.2018.]
Onoviran O, Taylor-Robinson D (1979) Detection of antibody against Mycoplasma mycoides subsp. mycoides in cattle by an enzyme-linked immunosorbent assay. Vet Rec 105:165-166.
Pfutzner H, Sachse K (1996) Mycoplasma bovisas an agent ofmastitis, pneumonia, arthritis and genital disorders in cattle. Rev Sci Tech Off Int Epiz 15:1477-1494.
Razin S, Yogev D, Noat Y (1998) Molecular biology and pathogenicity of mycoplasmas. Microbiol Mol Biol Rev 62:1094-1156.
Stipkovits L, Rady M, Glavits R (1993) Micoplasmal arthriris and meningitis in calves. Acta Vet Hung 41:73-88.
Tenk M(2005) Examination of Mycoplasma bovis infection in cattle. PhD thesis, Szent Istvan University.Budapest, Hungary. Aveilable online: http://www.huveta.hu/bitstream/handle/10832/134/TenkMiklosDissertation.pdf;jsessionid=0645DD4C2402838D570B23E53E713330?sequence=1 [accessed 05.09.2017. ]
Vidic B, Savic S, Grgic Z, Suvajdzic L, Prica N (2014) Seroepidemiological investigation of Mycoplasma bovis in calves. Arch Vet Med 7:3-9.
Vojinovic D, Vasic A, Zutic J, Djuricic B, Ilic Z, Jovicic D, Elezovic Radovanovic M (2014) Determination of Mycoplasma bovis specific antibodies in blood sera of asymptomatic carriers-calves in three farms in the Republic of Serbia by using indirect ELISA assay. J Hell Vet Med Soc 65:779-822.