Evaluation of the Effects of Different Natural Dietary Feed Additives on Performance and Intestinal Histomorphology in Quails

Published: Οκτ 25, 2022
2022-10-25 (1)
crypt depth essential oil gut health performance probiotic
Derya Yesilbag
Esra Abdullahoglu
Emin Urkmez
Aykut Acar
Deniz Asmaz
Mert Kara

The aim of the present study was to investigate the influence of a commercial probiotic and a commercial essential oil blend and their mixture, as a natural feed additive, on growth parameters live weight (LW), live weight gain (LWG), feed intake (FI), feed conversion ratio (FCR), carcass yield (CY) and small intestinal histomorphology of quails. A total of 200 1-day-old Japanese (Coturnix coturnix japonica) quails, including both males and females, were divided into four groups containing 50 quails and treated as follows: (1) a control treatment without medication (2) 18 g ton-1 probiotic;  (3) 300 g ton-1  essential oil blend  and (4) 18 g ton-1  probiotic plus 300 g ton-1 essential oil blend. The results of the research show that the additives added to quail diets do not have a significant effect (P > 0.05) on the performance parameters of LW, LWG, FI and FCR. However, significant differences (P < 0.05) were found in the CY, which is one of the performance parameters. A probiotic addition of 18 g ton -1 (treatment 1) to the quail diet resulted in a significant increase (P < 0.05) in carcass yield. Moreover, the addition of natural feed additives such as probiotics, essential oil blends and mixture to quail diets caused significant (P < 0.001) increases in villus heights and crypt depth in the duodenum and ileum segments. In addition, significant (P < 0.05) enlargements were found in the villus surface area in the experimental groups compared to the control group. In conclusion, in this study, it was determined that the addition of feed additives, which are developed as an alternative to antibiotics to the quail diet did not have a negative effect on performance and caused significant differences in the effective parameters in the evaluation of intestinal health.

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Abdel-Raheem SM, Abd-Allah SM, Hassanein KM (2012) The Effects of Prebiotic, Probiotic and Synbiotic Supplementation on Intestinal Microbial Ecology and Histomorphology of Broiler Chickens. Int J Agro Vet Med Sci 6(????): 277-289.
Al-Baadani HH, Abudabos AM, Al-Mufarrej SI, Alzawgari M (2016) Effects of Dietary Inclusion of Probiotics, Prebiotics and Synbiotics on Intestinal Histological Changes in Challenged Broiler Chickens. South African J Anim Sci 46 (2): 157–165.
AOAC (2000) Official Methods for Analysis. 17th ed. Maryland USA: Association of Official Analytical Chemists.
Awad WA, Ghareeb K, Abdel-Raheem S, Bohm J (2009) Effects of Dietary Inclusion of Probiotic and Synbiotic on Growth Performance, Organ Weights, and Intestinal Histomorphology of Broiler Chickens. Poult Sci 88(??): 49-56.
Cengiz SS, Yesilbag D, Meral Y, Cetin I, Biricik H (2015) Juniper Oil Improves Oxidative Stability and Eggshell and Albumin Quality of Quail Eggs. Br Poult Sci 56(1): 58-65.
Chichlowski M, Croom J, Mcbride B, Daniel L, Davis G, Koci M (2007) Direct-Fed Microbial PrimaLac and Salinomycin Modulate Whole-Body and Intestinal Oxygen Consumption and Intestinal Mucosal Cytokine Production in the Broiler Chick. Poult Sci 86(6): 1100-1106.
Choct M. (2009) Managing Gut Health through Nutrition. Br Poult Sci 50(1): 9-15.
Crosnier C, Stamataki D, Lewis J (2006) Organizing Cell Renewal in the Intestine: Stem Cells, Signals and Combinatorial Control. Nat Rev Genet 7: 349–359.
Culling CFA, Allinston RT, Barr WT (1985) Cellular Pathology Technique: 642-647 ed. Butterworths, London, UK: Connective Tissue.
Dawson B, Trapp RG (2001) Basic and Clinical Biostatistics. 3rd ed. New York, USA: Lange Medical Books.
Domingues CH, Santos ET, Castiblanco DC, De Quadros TCO, Petrolli TG (2014) Avaliação do Desempenho e Rendimento de Carcaça de Frangos de Corte Alimentados com Dietas Contendo Probiótico nas Diferentes Fases de Criação. Rev Agrocientífica 1(1): 7-16.
Du E, Wang W, Gan L, Li Z, Guo S, Guo Y (2016) Effects of Thymol and Carvacrol Supplementation on Intestinal Integrity and Immune Responses of Broiler Chickens Challenged with Clostridium Perfringens. J Animal Sci Biotechnol 7: 19.
Galdeanoa CM, Cazorlaa SI, Dumit JML, Vélez E, Perdigóna G (2019) Beneficial Effects of Probiotic Consumption on the Immune System. Ann Nutr Metab 74:115–124.
Garcia V, Catala-Gregori P, Hernandez F, Megias MD, Madrid J (2007) Effect of Formic Acid and Plant Extracts on Growth, Nutrient Digestibility, Intestine Mucosa Morphology and Meat Yield of Broilers. J Appl Poult Res 16: 555-562.
Giannenas I, Florou-Paneri P, Botsoglou NA, Christaki E, Spais AB (2005) Effect of Supplementing Feed with Oregano and/or a-Tocopheryl Acetate on Growth of Broiler Chickens and Oxidative Stability of Meat. J Anim Feed Sci 14(3):521-535.
Giannenas I, Florou-Paneri P, Papazahariadou M, Botsoglou NA, Christaki E, Spais AB (2003) Effect of Dietary Supplementation with Oregano Essential Oil on Performance of Broilers After Experimental Infection with Eimeria Tenella. Arch Anim Nutr 57(2): 99-106.
Giannenas I, Papaneophytou C, Tsalie E, Mavridis S, Triantafillou E, Kontopidis G, Tontis D (2014) Effect of Benzoic Acid and Essential Oil Compounds on Performance of Turkeys, Intestinal Microbiota, Intestinal Morphology and Antioxidant Status. Asian-Australas J Anim Sci 27(2): 225-236.
Gong H, Yang Z, Celi P, Yan L, Ding X, Bai S, Zeng Q, Xu S, Su Z, Zhuo Y, Zhang K, Wang J (2021) Effect of benzoic acid on production performance, egg quality,intestinal morphology, and cecal microbial community of laying hens. Poult Sci 100(1): 196-205.
Hanchi H, Mottowea W, Sebei K, Hammami R (2018) The Genus Enterococcus: Between Probiotic Potential and Safety Concerns—An Update. Front Microbiol 9: 1971. Issue not found
Jin LZ, Ho YW, Abdullah N, Jalaludin S (1998) Growth Performance, Intestinal Microbial Populations and Serum Cholesterol of Broilers Fed Diets Containing Lactobacillus Cultures. Poult Sci 77: 1259–1265.
Kassie GAM (2008) The Effect of Anise and Rosemary on Broiler Performance. Int J Poult Sci 7:243-245.
Lee KW, Kim J, Oh S, Kang CB (2015) Effects of Dietary Sanguinarine on Growth Performance, Relative Organ Weight, Cecal Microflora, Serum Cholesterol Level and Meat Quality in Broiler Chickens. J Poult Sci 52(1): 15-22.
Leeson S, Summers JD (2001) Nutrition of the Chicken. 4th ed. Guelph, ON: University Books.
Miles RD, Butcher GB, Henry PR, Littell RC (2006) Effect of Antibiotic Growth Promoters on Broiler Performance, Intestinal Growth Parameters, and Quantitative Morphology. Poult Sci 85: 476-485.
Mohammadi Z, Ghazanfari S,. Moradi MA (2014) Effect of supplementing clove essential oil to the diet on microflora population, intestinal morphology, blood parameters and performance of broilers Eur Poult Sci 78: 1-11.
Nosrati M, Javandel F, Camacho LM, Khusro A, Cipriano M, Seidavi A (2017) The Effects of Antibiotic, Probiotic, Organic Acid, Vitamin C, and Echinacea Purpurea Extract on Performance, Carcass Characteristics, Blood Chemistry, Microbiota, and Immunity of Broiler Chickens. J Appl Poultry Res 26 (2): 295-306.
NRC (1994) Nutrient Requirements of Poultry. 9th ed. Washington, DC: National Academy Press.
Pan D, Yu Z (2014) Intestinal Microbiome of Poultry and its Interaction with Host and Diet. Gut Microbes 5: 1088-19.
Patel SG, Raval AP, Bhagwat SR, Sadrasaniya DA, Patel AP, Joshi SS (2015) Effects of Probiotics Supplementation on Growth Performance, Feed Conversion Ratio and Economics of Broilers. J Anim Res 5(1): 155-160.
Pelicano ERL, Souza PA, Souza HBA, Figueuredo DFII, Boiago MMI, Carvalho SRI, Bordon VFI (2005) Intestinal Mucosa Development in Broiler Chickens Fed Natural Growth Promoters. Br J Poult Sci 7(4):221-229.
Peterson LW, Artis D (2014) Intestinal Epithelial Cells: Regulators of Barrier Function and Immune Homeostasis. Nat Rev Immunol 14(3): 141–153.
Ramos LSN, Lobes JB, Ribeiro MN, Silva FES, Merval RR, Albuquerque DMN (2014) Aditivos alternativos a antibióticos para frangos de corte no período de 22 a 42 dias de idade. Rev Bras Saúde Prod Anim 15(4): 897-906.
Salois MJ, Cady RA, Heskett EA (2016) The Environmental and Economic Impact of Withdrawing Antibiotics from US Broiler Production. J Food Distrib Res 47(1): 1-2.
Samanya M, Yamuachi K (2002) Histological Alterations of Intestinal Villi in Chickens Fed Dried Bacillus Subtilis var. Natto. Comp Biochem Physiol A Mol Integr Physiol 133(1): 95–104.
Sen S, Ingale SL, Kim YW, Kim JS, Kim KH, Lohakare JD, Kim EK, Kim HS, Ryu MH, Kwon IK, Chae BJ (2012) Effect of Supplementation of Bacillus Subtilis LS 1-2 to Broiler Diets on Growth Performance, Nutrient Retention, Caecal Microbiology and Small Intestinal Morphology. Res Vet Sci 93(1): 264–268.
Sevim B, Gümüş E, Harman H, Ayaşan T, Başer E, Altay Y, Akbulut K (2020) Effects of Dietary Rosemary Essential Oil on Growth Performance, Carcass Traits and Some Hematological Values of Chukar Partridge. Turkish JAF Sci Tech 8(2):430-435
Shargh MS, Dastar B, Zerehdaran S, Khomeiri M, Moradi A (2012) Effects of Using Plant Extracts and a Probiotic on Performance, Intestinal Morphology, and Microflora Population in Broilers. J Appl Poult Res 21(2): 201-208.
Shang Y, Kumar S, Oakley B, Kim WK (2018) Chicken Gut Microbiota: Importance and Detection Technology. Front Vet Sci 5:254.
Silva MAN, Barbosa Filho JAD, Silva CJM, Rosario MF, Silva IJO, Coelho AAD, Savino VJM (2007) Avaliação do estresse térmico em condição simulada de transporte de frangos de corte. R Bras Zootec 36 (4):1126-1130.
SPSS (1997) Statistical Software for Windows Version 7.5 Microsoft Windows Chicago, IL.
Souza LFAD, Araujo DN, Astoplhi JLDL, Ambiel AC, Santos LS, Carmo AJ, Silva PCG (2010) Probiótico e antibiótico como promotores de crescimento para frangos de corte. Colloq Agr 5(1) 33-39.
Tufarelli V, Baghban-Kanani P, Azimi-Youvalari S, Hosseintabar-Ghasemabad B, Slozhenkina M, Gorlov I, Seidavi A, Ayaşan T, Laudadio V (2021). Effects of Horsetail (Equisetum arvense) and Spirulina (Spirulina platensis) Dietary Supplementation on Laying Hens Productivity and Oxidative Status. Animals 11: 335.
Tzora A, Giannenas I, Karamoutsios A, Papaioannou N, Papanastasiou D, Bonos E, Skoufos S, Bartzanas T, Skoufos I (2017) Effects of Oregano, Attapulgite, Benzoic Acid and their Blend on Chicken Performance, Intestinal Microbiology and Intestinal Morphology. J Poult Sci 54(3): 218–227.
Yang X, Xin H, Yang C, Yang X (2018) Impact of Essential Oils and Organic Acids on the Growth Performance, Digestive Functions and Immunity of Broiler Chickens. Anim Nutr 4(4): 388–393.
Yesilbag D, Biricik H, Gezen SS, Meral Y (2013) Effect of a Rosemary and Oregano Volatile Oil Mixture on Performance, Egg Traits and Oxidative Stability of Eggs in Laying Quails. Br Poult Sci 54(2): 231-237.
Yesilbag D, Cengiz SS, Cetin I, Meral Y, Biricik H (2014) Influence of Juniper (Juniperus Communis) Oil on Growth Performance and Meat Quality as a Natural Antioxidant in Quail Diets. Br Poult Sci 55(4): 495-500.
Zeng Z, Zhang S, Wang H, Piao X (2015) Essential oil and aromatic plants as feed additives in non-ruminant nutrition: a review. J Anim Sci Biotechno (2015) 6(7): 2-10.
Zhai H, LIU H, Wang S, Wu J, Kluenter, AM (2018) Potential of Essential Oils for Poultry and Pigs. Anim Nutr 4(2): 179-186.
Zhang JJ, Piao XS, Huang DS, Wang JJ, Ma X, Ma YX (2009) Effects of Feed Particle Size and Feed Form on Growth Performance, Nutrient Metabolizability, and Intestinal Morphology in Broiler Chickens. Asian- Aust J Anim Sci 22(1):107–112.
Zhang LY, Peng QY, Liu YR, Ma QG, Zhang JY, Guo YP, Xue Z, Zha LH (2021) Effects of Oregano Essential Oil as an Antibiotic Growth Promoter Alternative on Growth Performance, Antioxidant Status, and Intestinal Health of Broilers. Poult Sci 100 (7):101-163.
Zulkifli I, Abdullah N, Azrin NM, HO YW (2000) Growth Performance and Immune Response of Two Commercial Broiler Strains Fed Diets Containing Lactobacillus Cultures and Oxytetracycline Under Heat Stress Conditions. Br Poult Sci 41(5):593–597.
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