Phenotypic and Molecular Characterization of Vibrio Species Isolated from Fish markets in Egypt

PDF
DOI: https://doi.org/10.12681/jhvms.27517
Keywords:
fish PCR Vibrio species
D.E.A. GOBARAH
Department of Bacteriology, Kafr El-Sheikh Regional Laboratory, Animal Health Research Institute, Agriculture Researches Center (ARC), Egypt
S.M. HELMY
Department of Microbiology, Faculty of Veterinary Medicine, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt
N.B. MAHFOUZ
Department of Fish diseases, Faculty of Veterinary Medicine, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt
H.A. FAHMY
Department of Biotechnology, Animal Health Research Institute, Dokki, Agriculture Research Center (ARC), Egypt
M.A.M. ABOU ZEID
Department of Bacteriology, Kafr El-Sheikh Regional Laboratory, Animal Health Research Institute, Agriculture Researches Center (ARC), Egypt
E.M. MOUSTAFA
Department of Fish diseases, Faculty of Veterinary Medicine, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt
Abstract

Vibriosis is considered a worldwide, threatening bacterial disease that affects mariculture, with high mortalities and severe economic losses. Some Vibrio species have been frequently involved in outbreaks of foodborne diseases worldwide. The Genus Vibrio includes over eighty-five species present in marine and natural habitats of seawater, and the species are widely distributed throughout the world. This work aimed to isolate Vibrio species from different markets in Kafr EL-Sheikh Governorate, representing 40% of Egypt’s fish production. Samples of Nile tilapia, (Oreochromis niloticus), grey mullet (Mugil cephalus) and African sharptooth catfish (Clarias gariepinus) were collected alive and examined for Vibrio species. Isolation and identification of Vibrio species were made using colonial morphology and biochemical characteristics, then confirmed using 16S rRNA gene-specific for the genus Vibrio and multiplex PCR using species-specific primers. 52 (34. 6%) Vibrio isolates were obtained from examined fishes. The highest incidence of Vibrio species was detected in C. gariepinus (64%), followed by M. cephalus (36%) and then O. niloticus (24%). In the case of C. gariepinus, V. alginolyticus was the most predominant species (32%), followed by V. fluvialis (12%), V. cholerae, V. parahaemolyticus (8%), and V. splendidus (4%). In the case of O. niloticus, the predominant Vibrio species were V. alginolyticus (12%), followed by V. parahaemolyticus (5. 33%), V. cholerae (4%), and then V. splendidus and anguillarum (1. 33%). In M. cephalus, V. alginolyticus also wasthe predominant species (14%), followed by V. cholerae (12%), V. parahaemolyticus, V. fluvialis (2%), and V. splendidus (2 %). V. alginolyticus, V. cholerae, and V. parahaemolyticus were found to produce PCR products of 737, 304, and 897 bp, respectively. This study highlights the incidence of Vibrio species in fish in Egypt.

Article Details
  • Section
  • Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
Download data is not yet available.
References
Abdelaziz M, Ibrahem MD, Ibrahim MA, Abu-Elala NM, Abdel-moneam DA (2017) Monitoring of different vibrio species affecting marine fishes in Lake Qarun and Gulf of Suez: Phenotypic and molecular characterization. Egypt. J. Aquat. Res, 43 (2): 141-146.
Abd El-Gaber GA, Naguib M and Abd El- Aziz ES (1997) Vibrio species infections to Oreochromisniloticus and Mugilcephalus. Sodium choloride tolerance, pathogenisity, serological relatedness and antibiogram sensitivity of recovered Vibrios. Vet. Med. J., Giza, 45 (1):87-99.
Adeleye IA, Daniels FV and Enyinnia VA (2010) Characterization and Pathogenicity of Vibrio spp. Contaminating Seafoods in Lagos, Nigeria. Internet Journal ofFood Safety 12: 1-9.
Alapide-Tendencia EV and Dureza LA (1997) Isolation of Vibrio species from Penaeusmonodon(Fabricius) with red disease syndrome. Aquaculture, 154(2):107-114.
Alonzo KHF, Cadiz RE, Traifalgar RFM and CorreVL Jr (2017)Immune responses and susceptibility to Vibrio parahaemolyticus colonization of juvenile Penaeus vannamei at increased water temperature. Aquac. Aquar. Conserv. Legis. (Bioflux), 10(5): 1238-1247.
Alsina M and Blanch AR(1994a) A set keys for biochemical identification of Vibrio sp. J. Appl. Bacteriol, 76(1):79-85.
Alsina M and Blanch AR (1994b) Improvement and update of a set of key for biochemical identification of Vibrio species . J. Appl. Bacteriol, 77(6): 719-721.
Al-Taee1 AMR, Khamees NR and Al-Shammari NAH (2017)Vibrio Species Isolated from Farmed Fish in Basra City in Iraq. J Aquac Res Development, 8 (2), 1-8.
Anzar R and Alarcon B (2008) On the specificity of PCR detection of Listeria monocytogenes in food: a comparison of published primers. Syst. Appl. Microbiol. 25(1):109-119.
Austin B & Austin DA (2016) Vibrios. Bacterial Fish Pathogens: Disease of Farmed and Wild Fish. Springer International Publishing. Austin B and Austin DA (1987) Bacterial fish pathogens, diseases in farmed and wild fish. 15th ed. Ellis Howrwood Limited, England, 263-287.
Begum M, Abu Ahmed A, Das M, and Parveen S (2010) Acomparative microbiological assessment of five types of selected fishes collected from two different markets. Advances in Biological Research. 4 (5):259-265.
Béné C, Arthur R, Norbury H, Allison, EH, Beveridge M, Bush S, Campling L, Leschen W, Little D, Squires D, Thilsted SH, Troell M, and Williams M (2016) Contribution of fisheries and aquaculture to food security and poverty reduction: Assessing the current evidence. World Dev, 79:177-196.
Chen Y, Liu XM, Yan JM, Li XG, Mei LL, Fa QM and Fa Y (2010) Foodborne pathogens in retail oysters in South China. BIOMED ENVIRON SCI. . 23:32-36.
De Menezes FGR, Neves SD, De Sousa OV, Vila-Nova CMVM, Maggioni R, Theophilo GND, Hofer E and Vieira RHSD (2014) Detection of virulence genes in environmental strains of Vibrio cholera from estuaries in northeastern Brazil. Rev. Inst. Med. Trop. Sao Paulo, 56(5): 427-432.
Di DYW, LEE A, Jang J, Han D, Hur H (2017) Season-Specific Occurrence of Potentially Pathogenic Vibrio spp. On the Southern Coast of South Korea. Appl Environ Microbiol, 83(3):
e02680-16.
Di pino A, Ciccarese G, Tantillo G, Catalano G and ForteVT (2005) A Collagenase-Targeted Multiplex PCR Assay for Identification of Vibrio alginolyticus, Vibrio cholerae, and Vibrio parahaemolyticus. J Food Prot., 68(1): 150-153.
El-Bouhy Z, El-Nobi G, El-Murr A, Abd El-Hakim S (2016) Study On Vibriosis In MugilCapito In El-Dakahlia And Damitta Governorates, Egypt. Abbassa Int. J. Aquat. 9 (1):19-35.
Elliot EL, Kaysner CA, Jackson L and Tamplin ML (1995) Vibrio cholerae, V. parahaemolyticus, V. vulnificus and other Vibrio spp, p. 9. 01-9. 27. In FDA Bacteriological Analytical Manual, 8th ed. AOAC International, Gaithersburg, MD.
FAO (Food and Agriculture Organization of the United Nations) (2016) National aquaculture sector overview. Malaysia. National aquaculture sector overview fact sheets. Fisheries and aquaculture information and statistics branch.
Fouz B, Alcaide E, Barrera R and Amaro C (2002) Susceptibility of Nile Tilapia (Oreochromisniloticus) to vibriosis due to Vibrio vulnificus bio type 2 (Serovar E). Aquaculture. 212(1-4): 21-30.
Ghenem L, Elhadi N, Alzahrani F and Nishibuchi M (2017) Vibrio parahaemolyticus: A review on distribution, pathogenesis, virulence determinants and epidemiology. Saudi J. Med. Med. Sci., 5(2): 93-103.
Haenen OLM, van ZantenE, Jansen R, Roozenburg I, Engelsma MY, Dijkstra A, Boers SA, Voorbergen-Laarman M, Möller AVM (2014) Vibrio vulnificus outbreaks in Dutch eel farms since 1996: strain diversity and impact. Dis. Aquat. Org, 108(3): 201-209.
Lee CT, Chen IT, Yang YT, KoTP, Huang YT, Huang JY, Lo CF (2015) The opportunistic marine pathogen Vibrio parahaemolyticus becomes virulent by acquiring a plasmid that expresses a deadly toxin. Proc Nat Acad Sci USA, 112(34): 10798-10803.
Levican A, Fisher JC, McLellan SL, Avendaño-Herrera R (2020) Microbial Communities Associated with Farmed Genypteruschilensis: Detection in Water Prior to Bacterial Outbreaks Using Culturing and High-Throughput Sequencing. Animals. 10(6): 1055.
Lipp EK, Huq A, Colwell RR (2002) Effects of global climate on infectious disease: The cholera model. ClinMicrobiol Rev, 15: 757-770.
Liu L, Ge M, Zheng X, Tao Z, Zhou S, Wang G (2016) Investigation of Vibrio alginolyticus, V. harveyi, and V. parahaemolyticus in large yellow croaker, Pseudosciaenacrocea(Richardson) reared in Xiangshan Bay, China. AQUACULT.
Luan X, Chen J, Zhang LiY and Hu G (2007) Expression and characterization of a metalloprotease from a Vibrio parahaemolyticus isolate. Can. J. Microbiol, 53(10):1168-1173.
Morris JGJ, Black RE (1985) Cholera and other vibrioses in the United States. N Engl J Med, 312(6):343-350.
Mosustafa M, Laila AM, Mahmoud M A, Soliman WS, El-gendy MY (2010) Bacterial infections affecting marine fishes in Egypt, j of American Science: 6(11): 603-612.
Nagasawa K, Cruz-Lacierda ER (2004) Diseases of Cultured Groupers. Southeast Asian Fisheries Development Center, Aquaculture Department, Iloilo, Philippines. pp. 81.
Noga EJ (2010) Fish disease: Diagnosis and treatment. 2nd ed., Walswort publishing Co., USA. PP. 149.
Nor-Amalina Z, Dzarifah MZ, Mohd-Termizi Y, Amal MNA, Zamri-Saad M and Ina-Salwany MY (2017) Phenotypic and genotypic characterization of Vibrio species isolates from Epinephelusspecies in Selangor, Malaysia. In: Proceedings of the International Conference on Advances in Fish Health, 4-6 April, UPM Serdang, Malaysia.
Percival SL, Williams DW (2014) Vibrio. Microbiology of Waterborne Diseases (Second Edition).
Radwan IA (1995) Bacteriological characterization of Gram-negative bacteria isolated from fishes. A thesis Ph. D. Microbiology. Fac. Vet. Med., Beni-Suef, Cairo Univ.
Sambrook J, Fritsh EF and M anniato TS (1989) Molecular cloning. A laboratory Manual. 2nd ED., Coldspring, Habor Laboratory Coldspring, New York.
Shahat AA, Mehana EE (2000)Vibrio anguillarumas a stress-born pathogen in cultured freshwater fish. Vet. Med. J. ; Giza, 48(1), 1-6.
Shyne Anand PS, Sobhana KS, George KC, Paul Raj R (2008) Phenotypic characteristics and antibiotic sensitivity of Vibrio parahaemolyticusstrains isolated from diseased groupers (Epinephelus spp. ). J. Mar. Biol. Ass. India, 50(1):1-6.
Tarr CL, Patel JS, Puhr ND, Sowers EG, Bopp C A, Strockbine NA(2007) Identification of Vibrio isolates by a multiplex PCR assay and rpoB sequence determination. J Clin Microbiol., 45(1):134-140.
Winfield IJ (2018) Sea Bass and Sea Bream A Practical Approach to Disease Control and Health Management. Journal of Fish Biology, 93:434- 434.
Yang Z, Jiao X, Zhou X, Cao G, Fang W, Gu R (2008) Isolation and molecular characterization of Vibrio parahaemolyticus from fresh, low-temperature preserved, dried and salted seafood products in two coastal areas of eastern ChinaInt J Food Microbiol, 125(3):279-285.
Zorrilla I, Moriñigo M, Castro D, Balebona M & Borrego J (2003) Intraspecific characterization of Vibrioalginolyticus isolates recovered from cultured fish in Spain. Journal of Applied Microbiology, 95, 1106- 1116.
Most read articles by the same author(s)