Essential oil in milk and calf starter feed for Simmental calves: Growth performance, hematological, and biochemical parameters


Published: Μαΐ 18, 2026
Updated: 2026-05-18
Versions:
2026-05-18 (2)
Keywords:
calf essential oil blend growth performance hemogram blood biochemical parameters
A Bal
Z Selçuk
Abstract

This study aimed to evaluate the effects of supplementing a commercial essential oil blend (EOB) to milk and calf starter feed (CS) on the growth performance, blood markers, oxidative stress markers, and fecal consistency of Simmental calves during the preweaning period. This study was conducted with a controlled experimental trial design. The treatments were as follows: (1) Control: CS and milk without EOB, (2) MEOB: Supplementation of milk with 0.06 mL of a commercial liquid product of EOB/L of milk, (3) CSEOB: Supplementation of CS with 1000 g of a commercial powder product of EOB/t of CS, and (4) MCSEOB: Both supplementation milk with 0.06 mL of commercial liquid product of EOB/L of milk and 1000 g of a commercial powder product of EOB/t in CS. Growth performance, feed intake, and health parameters were monitored over 56 days. EO supplementation did not significantly impact average feed intake or overall growth rate outcomes. However, calves receiving essential oils in both milk and CS exhibited a 6.58% increase in weight by weaning and demonstrated reduced fecal scores, indicating enhanced digestive health. Furthermore, supplementation enhanced markers related to growth, such as insulin-like growth factor-1 (IGF-1), and reduced oxidative stress. These findings suggested that EOB may serve as a natural alternative to antibiotics, promoting better health and productivity in calves. Nevertheless, further research is recommended to optimize EO formulations and determine their long-term effects on livestock performance and welfare.

Article Details
  • Section
  • Research Articles
Downloads
Download data is not yet available.
References
Abd El-Hafeez AM, Ali MAE, Abu El-Hamd MA, et al., 2014. Productive
performance, immune status and metabolic activity of suckling
bovine calves treated with Nigella sativa oil. Egypt J Agric Res
:1561–74.
Ahmed AA, Bassuony NI, Awad SES, et al., 2009. Adding natural juice
of vegetables and fruitage to ruminant diets (B) nutrients utilization,
microbial safety and immunity, effect of diets supplemented with
lemon, onion and garlic juice fed to growing buffalo calves. World
J Agric Sci 5:456–65.
Akbarian‐Tefaghi M, Ghasemi E and Khorvash M, 2018. Performance,
rumen fermentation and blood metabolites of dairy calves fed starter
mixtures supplemented with herbal plants, essential oils or monensin.
J Anim Physiol Anim Nutr 102:630–38.
Allen HK, Levine UY, Looft T, et al., 2013. Treatment, promotion, commotion:
antibiotic alternatives in food-producing animals. Trends
Microbiol 21:114–19.
AOAC, 2006. Official Method of Analysis, 18th ed.; Association of
Official Analytical Chemist: Washington, DC, USA.
Benchaar C, Calsamiglia S, Chaves AV, et al., 2008. A review of
plant-derived essential oils in ruminant nutrition and production.
Anim. Feed Sci Technol 145:209–228.
Busquet M, Calsamiglia S, Ferret A, et al., 2006. Plant extracts affect in
vitro rumen microbial fermentation. J Dairy Sci 89:761–71.
Campolina JP, Coelho SG, Belli AL, et al., 2021. Effects of a blend of
essential oils in milk replacer on performance, rumen fermentation,
blood parameters, and health scores of dairy heifers. PLoS ONE
:e0231068.
Campolina JP, Coelho SG, Belli AL, et al, 2023. Potential benefits of
a blend of essential oils on metabolism, digestibility, organ development
and gene expression of dairy calves. Sci Rep 13:3378.
Castillejos L, Calsamiglia S, Ferret A, et al., 2007. Effects of dose and
adaptation time of a specific blend of essential oil compounds on
rumen fermentation. Anim Feed Sci Tech 132:186–201.
Christaki E, Giannenas I, Bonos E, et al., 2020. Innovative uses of aromatic
plants as natural supplements in nutrition. In Feed Additives
(Florou-Paneri P, Christaki E and Giannenas I, eds.): Academic
Press: Elsevier Inc, pp:19–34.
Clemmons DR, 2012. Metabolic actions of insulin-like growth factor-I
in normal physiology and diabetes. Endocrinol Metab Clin North
Am 41(2):425-43.
Coelho MG, da Silva AP, de Toledo AF, et al., 2023. Essential oil blend
supplementation in the milk replacer of dairy calves: Performance
and health. PLoS ONE 18:e0291038.
Ebrahimi MA, Sobhanirad S and Bayat AR, 2018. Effects of Ajwain
(Trachyspermum ammi) and Thyme (Thymus vulgaris) oils on nutrients
digestibility, blood parameters and growth performance of
Brown Swiss neonatal calves. Iran J Appl Anim Sci 8:387–95.
Froehlich KA, Abdelsalam KW, Chase C, et al., 2017. Evaluation of
essential oils and prebiotics for newborn dairy calves. J Anim Sci
:3772–82.
Gilling DH, Ravishankar S and Bright KR, 2019. Antimicrobial efficacy
of plant essential oils and extracts against Escherichia coli. J
Environ Sci Health A Tox Hazard Subst Environ Eng 54:608–16.
Hassan AA, Salem AZM, Kholif AE, et al., 2016. Performance of crossbred
dairy Friesian calves fed two levels of Saccharomyces cerevisiae:
intake, digestion, ruminal fermentation, blood parameters and
faecal pathogenic bacteria. J Agric Sci 154:1488–98.
Izzaddeen SI and Kaygısız A, 2018. Effect of essential oil of Laurel
(Laurus nobilis L.) on performance, blood and fecal parameters of
Holstein calves during suckling period. Pak J Zool 50:1087–96.
Jeshari M, Riasi A, Mahdavi AH, et al., 2016. Effect of essential oils
and distillation residues blends on growth performance and blood
metabolites of Holstein calves weaned gradually or abruptly. Livest
Sci 185:117–22.
Kazemi-Bonchenari M, Falahati R, Poorhamdollah M, et al., 2018.
Essential oils improved weight gain, growth and feed efficiency
of young dairy calves fed 18 or 20% crude protein starter diets. J
Anim Physiol Anim Nutr 102:652–61.
Kerr DE, Laarveld B, Fehr MI, et al., 1991. Profiles of serum IGF-I
concentrations in calves from birth to eighteen months of age and
in cows throughout the lactation cycle. Can J Anim Sci 71:695–705.
Kolling GJ, Panazzolo DM, Gabbi AM, et al., 2016. Oregano extract
added into the diet of dairy heifers changes feeding behavior and
concentrate intake. Sci World J 1–6.
Kuralkar P and Kuralkar SV, 2021. Role of herbal products in animal
production–an updated review. J Ethnopharmacol 278.
Lee-Rangel HA, Mendoza-Martinez GD, Martínez-García JA, et al.,
An Indian polyherbal phytogenic source improved blood
serum biochemistry and immune response of dairy calves. Food
Agric Immunol 33:97–112.
Liu T, Chen H, Bai Y, et al., 2020. Calf starter containing a blend
of essential oils and prebiotics affects the growth performance of
Holstein calves. J Dairy Sci 103:2315–23.
Lorenz, I, Fagan, J and More SJ, 2011. Calf health from birth to weaning.
II. Management of diarrhoea in pre-weaned calves. Irish Veterinary
Journal 64(9).
Luo Z, Liu T, Li P, et al., 2023. Effects of essential oil and/or encapsulated
butyrate on fecal microflora in neonatal Holstein calves.
Animals 13:3523.
Luo Z, Liu T, Cairang D, et al., 2024. Oregano essential oil as a natural
plant additive affects growth performance and serum antibody levels
by regulating the rumen microbiota of calves. Animals 14:820.
Lykkesfeldt J and Svendsen OL, 2007. Oxidants and antioxidants in
disease: Oxidative stress in farm animals. Vet J 173:502–11. Marwan
AA and Mousa SA, 2021. Influence of basil oil as an essential oil
on buffalo calves performance, digestibility, haemobiochemical
profile and rumen fermentation indicators. Egyptian J Nutrition
and Feeds 24:211–21.
McGuirk SM, 2008. Disease management of dairy calves and heifers.
Vet Clin North Am Food Anim Pract 24:139–53.
Negi PS, 2012. Plant extracts for the control of bacterial growth: Efficacy,
stability and safety issues for food application. Int J Food
Microbiol 156:7–17.
Özek T, Tabanca N, Demirci F, et al., 2010. Enantiomeric distribution of
some linalool containing essential oils and their biological activities.
Rec Nat Prod 4:180–92.
Özen E, 2022. Süt Emen Buzağılara Kritik Besleme Dönemlerinde Çörek
Otu Tohumu Yağı İçirilmesinin Performans ve Kan Parametreleri
Üzerine Etkisi. Doctoral Thesis, Aydın Adnan Menderes University,
Health Sciences Institute, Department of Animal Nutrition and
Nutritional Diseases, Aydın.
Özkaya S, Erbaş S, Özkan O, et al., 2018. Effect of supplementing
milk replacer with aromatic oregano (Oreganum onites L.) water
on performance, immunity and general health profiles of Holstein
calves. Anim Prod Sci 58:1892–1900.
Özkaya S, Pigamov F, Erbaş S, et al., 2024. The effect of ginger (Zingiber
officinale L.) liquid extract on growth, immune response,
antioxidant defence mechanism, and general health of Holstein
calves. Trop Anim Health Prod 56:119.
Pan J, Zhu Y, Abdel-Samie MA, et al., 2023. Biological properties of
essential oil emphasized on the feasibility as antibiotic substitute
in feedstuff. Grain Oil Sci Technol 6:10–23.
Quigley JD, Caldwell LA, Sinks GD, et al., 1991. Changes in blood glucose,
nonesterified fatty acids, and ketones in response to weaning
and feed intake in young calves. J Dairy Sci 74:250–57.
Ranade R, Talukder S, Muscatello G, et al., 2014. Assessment of oxidative
stress biomarkers in exhaled breath condensate and blood of
dairy heifer calves from birth to weaning. The Veterinary Journal
:583–87.
Ranjan R, Naresh R, Patro RC, et al., 2006. Erythrocyte lipid peroxides
and blood zinc and copper concentrations in acute undifferentiated
diarrhoea in calves. Veterinary Research Communications
:249–54.
Renaud DL, Buss L, Wilms JN, et al., 2010. Technical note: Is fecal
consistency scoring an accurate measure of fecal dry matter in dairy
calves? J Dairy Sci 103:10709–14.
Ritt LA, Orso C, Silveira AK, et al., 2023. Oregano extract fed to preweaned
dairy calves. Part 1: Effects on intake, digestibility, body
weight, and rumen and intestinal bacteria microbiota. Livestock
Science 269:105165.
Rossi B, Toschi A, Piva A, et al., 2020. Single components of botanicals and nature-identical compounds as a non-antibiotic strategy to
ameliorate health status and improve performance in poultry and
pigs. Nutr Res Rev 33:218–34.
Sánchez N, Lee-Rangel HA, Martínez-Cortés I, et al., 2021. A polyherbal
phytogenic additive improved growth performance, health, and
immune response in dairy calves. Food Agr Immunol 32:482–98.
Santos FHR, de Paula MR, Lezier D, et al., 2015. Essential oils for
dairy calves: Effects on performance, scours, rumen fermentation
and intestinal fauna. Animal 9:958–65.
Seifzadeh S, Mirzaei Aghjehgheshlagh F, Abdibenemar H, et al., 2017.
The effects of a medical plant mix and probiotic on performance and
health status of suckling Holstein calves. Ital J Anim Sci 16:44–51.
Seirafy H and Sobhanirad S, 2017. Effects of oregano (Origanum vulgare)
and thyme (Thymus vulgaris) oils on growth performance
and blood parameters in Holstein suckling calves. Iran J Appl Anim
Sci 7:585–93.
SPSS. IBM Corp. Released 2012. IBM SPSS Statistics for Windows,
Version 21.0. Armonk, NY: IBM Corp.
Stefańska B, Katzer F, Golińska B, et al., 2022. Different methods of
eubiotic feed additive provision affect the health, performance,
fermentation, and metabolic status of dairy calves during the preweaning
period. BMC Vet Res 18:138.
Steiner Z, Benak S, Samardžija M, et al., 2024. The effect of different
forms of solid feed on biochemical parameters in blood plasma of
calves. Acta Vet-Beograd 74:444–59.
Suarez-Mena FX, Hu W, Dennis TS, et al., 2017. β-Hydroxybutyrate
(BHB) and glucose concentrations in the blood of dairy calves as
influenced by age, vaccination stress, weaning, and starter intake
including evaluation of BHB and glucose markers of starter intake.
J Dairy Sci 100:2614–24.
Svensson C and Hultgren J, 2008. Associations between housing, management,
and morbidity during rearing and subsequent first lactation
milk production of dairy cows in southwest Sweden. J Dairy Sci
:1510–18.
Urie NJ, Lombard JE, Shivley CB, et al., 2018. Preweaned heifer management
on US dairy operations: Part V. Factors associated with
morbidity and mortality in preweaned dairy heifer calves. J Dairy
Sci 101:9229–44.
Ünlü HB and Erkek R, 2013. Effects of oregano and garlic essential
oils on performance of calves and some parameters of faeces and
blood. Ege Üniv Ziraat Fak Derg 50:299–310.
Volpato A, Crescencio RB, Tomasi T, et al., 2019. Phytogenic as feed
additive for suckling dairy calves’ has a beneficial effect on animal
health and performance. An Acad Bras Cienc 91:e20180747.
Wang J, Deng L, Chen M, et al., 2024. Phytogenic feed additives as
natural antibiotic alternatives in animal health and production: A
review of the literature of the last decade. Anim Nutr 17:244-64
Windeyer MC, Leslie KE, Godden SM, et al., 2014. Factors associated
with morbidity, mortality, and growth of dairy heifer calves up to
months of age. Prev Vet Med 113:231–40.