@article{BOUGIOUKLIS (Π. ΜΠΟΥΠΟΥΚΛΗΣ)_GEORGOPOULOU (Ι. ΓΕΩΡΓΟΠΟΥΛΟΥ)_IORDANIDIS (Π. ΙΟΡΔΑΝΙΔΗΣ)_2017, title={Determination of breakthrough maternal antibodies titres by two intermediate and two intermediate plus vaccine strains against Gumboro disease, in commercial broilers}, volume={55}, url={https://ejournals.epublishing.ekt.gr/index.php/jhvms/article/view/15097}, DOI={10.12681/jhvms.15097}, abstractNote={<p><span lang="EN-US">Two field trials (termed A and B) were carried out on 4 farms (groups) with 20,000 broilers each. The groups in A and </span><span>Β</span><span lang="EN-US"> were named Al, A2, A3, A4 and Bl, B2, B3, B4, respectively. The chicks in A were hatched from breeders of 39, 43, 46, and 56 weeks-old and in </span><span>Β</span><span lang="EN-US"> from 27, 31, 52, and 54 weeks-old. One vaccination to each group was administered against Gumboro in drinking water, in each trial. Intermediate plus vaccines I and II were used in groups 1 and 2 and intermediate I and II in groups 3 and 4. In A the vaccinations were conducted when the chicks were 15-days-old and in </span><span>Β</span><span lang="EN-US"> at 19-days-old. In both trials blood samples were collected at 8, 15, 19, 23, 30 and 37 days of age. Serum </span><span lang="EN-US">examinations by ELISA established that in groups Al, A2, A3 and A4 vaccinations were carried out in the presence of mean maternal antibody titres 7.1 log</span><span lang="EN-US">2</span><span lang="EN-US">, 7.2 log</span><span lang="EN-US">2</span><span lang="EN-US">, 6.3 log</span><span lang="EN-US">2 </span><span lang="EN-US">and 6.4 log</span><span lang="EN-US">2</span><span lang="EN-US">, respectively. A regression of antibodies up to 37 days were observed in these 4 </span><span lang="EN-US">groups. In groups Bl,  B2, B3 and B4 the vaccinations were carrie out in the presence of mean maternal antibody titres 7.3 log</span><span lang="EN-US">2</span><span lang="EN-US">, 7.4 log</span><span lang="EN-US">2</span><span lang="EN-US">,3.2 log</span><span lang="EN-US">2 </span><span lang="EN-US">and 3.3 log</span><span lang="EN-US">2</span><span lang="EN-US">, respectively. A strong serological response from 30 days was observed in these 4 groups. It is remarkable that intermediate plus vaccines to 7.3log</span><span lang="EN-US">2 </span><span lang="EN-US">and 7.41og</span><span lang="EN-US">2 </span><span lang="EN-US">mean maternal antibody titres caused active immune response in groups Bl and B2, respectively, contrary to what happened to similar titres in groups Al and A2. This important observation showed that age may be one of the main factors that influences the effectiveness of vaccinations, as well as the interference of maternal antibodies and the virulence of vaccines strains. The results of both field trials indicate that both intermediate vaccines at 15-days-old did not breakthrough mean maternal antibody titres higher than 6.3 log</span><span lang="EN-US">2</span><span lang="EN-US">, whereas intermediate plus vaccines succeded to breakthrough mean maternal antibody titres lower than 7.4 log</span><span lang="EN-US">2 </span><span lang="EN-US">at 19-days-old.</span></p>}, number={3}, journal={Journal of the Hellenic Veterinary Medical Society}, author={BOUGIOUKLIS (Π. ΜΠΟΥΠΟΥΚΛΗΣ) P. and GEORGOPOULOU (Ι. ΓΕΩΡΓΟΠΟΥΛΟΥ) I. and IORDANIDIS (Π. ΙΟΡΔΑΝΙΔΗΣ) P.}, year={2017}, month={Dec.}, pages={217–225} }