@article{Cebeci_2022, title={Prevalence, Characterization and PFGE profiles of multidrug resistance extended spectrum β-lactamase producing Escherichia coli strains in animal-derived food products from public markets in Eastern Turkey}, volume={73}, url={https://ejournals.epublishing.ekt.gr/index.php/jhvms/article/view/29251}, DOI={10.12681/jhvms.29251}, abstractNote={Multidrug-resistant (MDR) Extended-spectrum β-lactamase (ESBL)-producing<em> Escherichia coli</em> (<em>E. coli</em>) has increased markedly in recent years, which has currently posed a major challenge in antimicrobial treatments  and raised concerns regarding possible transfer of such bacteria through the food chain. The aim of this research was to investigate the prevalence of ESBL-producing <em>E. coli</em> in milk, cheese and meat samples and to determine their virulence, pathotype, serotype, antibiotic resistance and genetic relatedness. A total of 300 food samples  were purchased from public markets in different districts of Giresun city. Five (1.6%) of 300 food samples resulted positive for ESBL-producing <em>E. coli</em> isolation. β-Lactamase-encoding genes of the CTX-M (20%), TEM (40%), and SHV (20%) groups were detected singly or in combination. Five ESBL-producing <em>E. coli </em>isolates were further analysed for the presence of virulence genes. Virulence factor genes detected were <em>hlyA</em> (20%), <em>ehlyA</em> (20%), <em>iutA</em> (60%), <em>iucD</em> (40%), <em>fimH</em> (100%), <em>kpsMTII</em> (100%) and <em>traT</em> (100%). Of 130 E.coli isolates, 5 ESBL-producing <em>E. coli</em> strains (totally 3.8%) were isolated, including 3 EHEC (60%). No EIEC, ETEC, EPEC, DAEC or EAggEC3  was detected. In PCR, three <em>stx2</em> (60%) and one <em>flicH7</em> (20%) encoding genes were found in ESBL-producing <em>E. coli</em> isolates. In addition, genes encoding Shiga toxins were detected in 3 of 5 isolates, three isolates (60%) encoded O128 serotype.The antibiotic susceptibility test of positive isolates showed resistant to cefuroxime, trimethoprim/ sulfamethoxazole, cefazolin, streptomycin, ceftriaxone, tetracycline, ampicillin and trimethoprim. Most of ESBL-producing <em>E. coli</em> isolates showed 80% MDR phenotypes against at least four classes of antibiotics. Specific-PCR detection of antibiotic resistance genes showed the prevalence of the <em>tet</em>A gene in most of the isolates (80%), followed by <em>dfrA, </em><em>qnr</em>, <em>aadA1</em> and <em>sul1</em>. PFGE results show that the isolates from different districts presented no clonal relatedness. This is the first report of the characteristics of multidrug resistance ESBL-producing shigatoxigenic<em> E. coli</em>  in dairy and meat products in a local city in Turkey. Our findings indicate that dairy and meat products could be reservoirs of MDR ESBL-producing <em>E. coli</em> strains that were possessed several virulence factors and may be a cause of concern for human health.}, number={3}, journal={Journal of the Hellenic Veterinary Medical Society}, author={Cebeci, T}, year={2022}, month={Nov.}, pages={4633–4644} }