TY - JOUR AU - RADY, M. AU - EZZ-EL-DIN, N.A. AU - MOHAMED, K.F. AU - NASEF, S. AU - SAMIR, A. AU - ELFEIL, W.K. PY - 2020/07/01 Y2 - 2024/03/28 TI - Correlation between ESβL Salmonella Serovars Isolated from Broilers and their Virulence Genes JF - Journal of the Hellenic Veterinary Medical Society JA - J Hellenic Vet Med Soc VL - 71 IS - 2 SE - Research Articles DO - 10.12681/jhvms.23645 UR - https://ejournals.epublishing.ekt.gr/index.php/jhvms/article/view/23645 SP - 2163-2170 AB - <p><em>Salmonell</em>a is considered to be one of the major poultry bacterial pathogens. The poultry species are one of the main reservoirs for the human types, thus serving as public health hazards. The development of drug resistant genes and multidrug resistant types from <em>Salmonell</em>a has increased recently. This current study was undertaken to estimate the correlation between extended spectrum beta-lactamase multidrug resistant (<em>ES</em><em>β</em><em>L</em>) <em>Salmonella </em>serovars isolated from broilers and their virulence genes. Two hundred and forty samples were collected from clinically diseased broilers chicks (showed disorders of the intestinal tract) and examined for the presence of <em>Salmonella </em>isolates according to ISO 6579: 2002 and ISO. 6579-3:2014 Fifty <em>Salmonella </em>isolates were isolated with an incidence of 20.8%. Isolates of <em>Salmonella </em>were serotyped as follows: 25 <em>S. </em>Kentucky, 9 <em>S. </em>Infantis<em>, </em>6 <em>S. </em>Enteritidis, 4 <em>S. </em>Heidelberg<em>, </em>and one isolates per serovars <em>S. </em>Labadie<em>, S. </em>Typhi<em>, S. </em>Agona<em>, S. </em>Pullorum<em>, S. </em>Newport and <em>S. </em>Virginia. AST (antimicrobial susceptibility testing) showed that high percentage of isolates were resistant to all Ampicillin (90%), Nalidixic acid (88%), Sulfamethoxazole + Trimethoprim (82%) and Tetracycline (82%). Approximately 86% of the isolates demonstrated multiple resistance, of which 18.75% and 25% were resistant to three and four antimicrobial types, respectively. Phenotypic detection of <em>ES</em><em>β</em><em>L</em>s by using screening test (<em>Cefinase</em>®) and confirmatory test by using combined disk diffusion test revealed that 32% of isolates were positive for both tests with 20% similarity and 12% diversity between the two tests. Molecular characterization of some <em>ES</em><em>β</em><em>L</em>s genes (<em>bla</em>TEM, <em>bla</em>CTX, <em>bla</em>OXA, <em>bla</em>CMY and <em>bla</em>SHV) and some virulence genes (<em>inv</em>A<em>, avr</em>A<em>, sop</em>B<em>, bcf</em>C<em>, stn </em>(was done using PCR. The results showed that all the <em>ES</em><em>β</em><em>L</em>s positive serovars were positive for amplification of all tested virulence genes and noticed that all the isolates were negativefor <em>bla</em>CMY gene. The present study suggests that virulent <em>ES</em><em>β</em><em>L Salmonella </em>serovars could infect broilers and should be taken into consideration as an important bacterial pathogen affecting poultry.</p> ER -