TY - JOUR AU - Komal, M AU - Afshan, K AU - Zafar, S AU - Firasat, S AU - Qayyum, M PY - 2022/11/09 Y2 - 2024/03/29 TI - Seroprevalence of Fasciola-infection using Enzyme-linked immunosorbent assay in large ruminants from Pakistan JF - Journal of the Hellenic Veterinary Medical Society JA - J Hellenic Vet Med Soc VL - 73 IS - 3 SE - Research Articles DO - 10.12681/jhvms.26436 UR - https://ejournals.epublishing.ekt.gr/index.php/jhvms/article/view/26436 SP - 4309-4316 AB - <p>Fasciolosis, caused by liver fluke species of the genus <em>Fasciola, </em>are well recognized because of its high veterinary impact. Stool examination for <em>Fasciola </em>eggs is not a sensitive method, and limited efforts to find a reliable and cheaper means of detection are available. The present study aimed to develop rapid diagnostic ELISA test against fasciolosis.<strong> </strong>The excretory/secretory (ES) and somatic (SA) products of <em>Fasciola</em> helminths were analysed using polyacrylamide gel electrophoresis (PAGE). Immunogenicity was evaluated by immunoblotting using hyperimmune sera raised in rabbits and seroprevalence was determined by indirect ELISA. <strong> </strong>The results of SA antigen of <em>Fasciola </em>species showed polypeptide bands ranged from 10kDa-100kDa, while ES antigen of <em>Fasciola </em>showed bands of 15kDa-55kDa. The immunoblotting results showed the most prominent bands against ES antibodies were 25, 35, 55-70, 100 and 250 kDa and SA antigens showed 10, 15-25, 35, 70, 100 and 250 kDa polypeptide bands. The sensitivity and specificity of developed indirect ELISA for SA antigens was 95.45% and 87.1%, while for ES antigens was 100% and 77.42% respectively. The overall seroprevalence recorded for fascioliasis based on SA antigen was 39.8% and 29.8% for ES antigen. The fasciolosis did not show significant association with host type, sex and age groups of examined animals, however significantly higher infection was found in months of September and October. The result provides sensitive in house immunodetection assay for diagnosis of fasciolosis alternative to commercial kits with high import cost. <strong></strong></p> ER -