Changes in the total protein, carbohydtate and lipid contents in selected tissues of silkworm, Bombyx mori L. under the influence of a juvenoid R394

PDF
Published: Jan 8, 2006
DOI: https://doi.org/10.12681/eh.11624
Keywords:
Bombyx mori cocoon parameters juvenoid R394 larval weight silk gland weight
K. Sashindran Nair
Central Sericultural Research & Training Institute, Karnataka, INDIA
Jula S. Nair
Central Sericultural Research & Training Institute, Karnataka, INDIA
V.A. Vijayan
Department of Studies in Zoology, Manasagangotri, Karnataka, INDIA
Abstract
A juvenoid. R394 (Ethyl 9-cyclohexyl-3,7-dimethyl-2,4-nonadienoate) was applied topically to 5th instar silkworm, Bombyx mori L. larvae (Hybrid: KA x NB4D2) at a dose of 0.039 nl/larva at 24, 48, 72 and 96 h, for silk yield improvement. Three major selected tissues viz., posterior silk gland (PSG), haemolymph and fat body were collected from fully-grown larvae and the total protein, total carbohydrate and total lipid contents were estimated following standard procedures. The result indicated that the content of these primary metabolites varied significantly in the selected tissues depending on the time of juvenoid application. The highest protein content was observed in the haemolymph and silk gland in the larvae treated at 72 h whereas the fat body protein content was lowest for the same treatment. The total carbohydrate was recorded lowest in the 72 h treated larvae as against the highest in the control both in haemolymph and fat body with no significant change in PSG. The total lipid content did not show any notable variation in the concentration on juvenoid administration except in the silk gland treated up to 72 h which showed a decline. The results indicate that the juvenoid induces tissue-specific responses in terms of turnover in primary metabolites which commensurates with the corresponding changes observed in the cocoon weight and cocoon shell weight.
Article Details
  • Section
  • Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons 4.0 license.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal. Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
Download data is not yet available.
References
Akai, Η., Κ. Kimura., M. Kiuchi and Α. Shibukawa, 1985. Increase of silk production by repeated treatments with a juvenile hormone analogue. J. Serie. Sci. Jpn. 54(4): 297-299.
Carrol, N.V., R.W. Longley and J.H. Rose. 1956. Glycogen determination in the liver and muscle by use of anthrone reagent. J. Biol. Chem. 220: 583-593.
Chen, P.S. 1985. Amino acid and protein metabolism. In Comprehensive Insect Physiology, Biochemistry and Pharmcoclogy. Vol. 10. Kertkut, G. A. and Gilbert L. I. (Eds.) Pergamon Press. Oxford, ρ 177-218.
Chowdhary, S.K., P.S. Raju and R.K. Ogra. 1990. Effect of JH analogues on silkworm, Bombyx mori L., growth and development of silk gland. Sericologia. 30: 155-165.
Folch, J.M., P. Lees and G.H. Stane-Stanely. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226: 497- 509.
Gilbert, L.I. 1967. Lipid metabolism and function in insects. In Advances in Insect Physiology. Vol. 4. Eds. Beament, J. W. L., Trehernc, J. E. and Wigglesworth, W. Β. Academic Press, New York, ρ 69-211.
Gururaj, C.S., B.M. Sekharappa and S.K. Sarangi. 1999. Metabolic profile of the target tissues in silkworm Bombyx mori infected with BmNPV. Indan J. Serie. 38: 89-94.
Harper H.A., V.W. Rodwell and P.A. Mayes. 1993. Review of physiological chemistry. Lange Medical Publication. Los Altos, California, ρ 131-182.
Hugar, LI. and B.B. Kaliwal. 1998. Effect of Benzyl-6-aminopurine and indole -3- acetic acid on the biochemical changes in the fat body and haemolymph of the bivoltine silkworm, Bombyx, morì L. Bull. Sericult, Res. 9: 63-67.
Lowry, O.H., N.J. Rosebrough, AL. Farr and R.J. Randal. 1951. Protein measurement with folin- phenol reagent. J. Biol. Chem. 193:265-275.
Muroga, A,, M. Nakajima, S, Aomori, Y. Ozawa and M Nihmura. 1975. Utilization of the synthetic juvenile hormone analog to the silkworm rearing on mulberry leaves. J. Sericult. Sci. Jpn., 44 (4), 267- 273
Nair, K.S., V.A. Vijayan, J.S. Nair and K. Trivedy. 1999. Juvenilomimic compounds for enhanced productivity in silkworm. Bombyx mori L. - A screening. Indian J. Serie. 38: 119-124.
Nair, K.S., V.A. Vijayan, J.S. Nair, K. Trivedy and P.K. Chinya. 2002, Hormctic influence on silkworm, Bombyx mori L of a phytojuvenoid, ω- formyl longifolenc oxime propargyl ether. Insect Sci. Applic. 22 (2): 145-150.
Nair, K.S., V.A. Vijayan, K. Trivedy and J.S. Nair. 2001, Improvement in the Commercial Traits of Silkworm, Bombyx mori L. by Administration of a Juvenoid, R394. Int. J. Industrial Entomol. 3(2): 169-175.
Nakayama, S., S. Fuji and R. Yamamoto. 1990. Changes in activities of glycosidases in the haemolymph of the silkworm, Bombyx mori, during larval development. J. Serie. Sci. Jpn. 59(6): 443-451.
Nath, B.S.. A. Suresh, B.M. Vanna and R.P.S. Kumar. 1997. Changes in protein metabolism in haemolymph and fat body of the silkworm Bombyx mori (Lepidopiei a: Bombycidae) in response to organophosphorous insecticide toxicity. Ecotoxicol. Environ. Safely. 36: 169-173.
Reddy, K.V.R., S.B. Magadum, O.K. Remadevi, K.V. Benchamin and R.K. Datta. 1992. Biochemical action of vertebrate hormone on the silk gland, fat Bombyx morì L. J. Reprod. Biol. Comp. Endocrinol. 4(2): 82-88.
Riddiford, L.M. and J.W. Truman. 1978. Biochemistry of insect hormones and insect growth regulators. In Biochemistry of Insects. Rockstein, M. (Ed) Academic Press., New York, ρ 308-357.
Sehnal, F. and H. Akai. 1990. Insect silk glands: Their types, development and function and effects of environmental factors and morphogenetic hormones on them. Int. J. Insect Morphol. Embryol. 19(2): 79-132.
Thomas, K.K. and J.L. Nation. 1966. RNA, protein and uric acid content of body body and gonads of silkworm tissues of Periptaneta americana L. as influence by corpora aliata during ovarian devlopment. Biol. Bull. 130: 442-449.
Tojo, S., K. Kiguchi and S. Kimura. 1981, Hormonal control of storage protein synthesis, and uptake by the fat body, in the silkworm. Bombyx mori. J. Insect Physiol., 27(7): 491-497.