| More

Effect of SSP – 11 on Weight and Tissue Composition of 4th Instar Larvae of Silkworm, Bombyx mori

Views: 210 Downloads: 206
K. Venkata Rami Reddy, K. Sashindran Nair, S. Β. Magadum, R. Κ. Datta
K. Venkata Rami Reddy, K. Sashindran Nair, S. Β. Magadum, R. Κ. Datta


The anti-juvenoid, SSP-11 ((E) 4-chloro-a, a, a-trifluro-N [ 1 (1 H-imidazole- 1 –y1)- 2-propoxy ethylyledene] O, toludine) was used to induce trimoulters from tetramoulter larvae of the silkworm, Bombyx mori (race NB7). The compound was fed for two days through an artificial diet at the concentration of 200 ppm to newly ecdysed 4th stadium larvae. Body and silkgland fresh weight were recorded daily in the 4th larval stadium and protein profiles of haemolymph, fat body and silkgland were determined at the end of the stadium. In larvae treated with SSP-11, feeding period in stadium 4 was prolonged by 3-4 days and 80-85% of the larvae started to spin as trimoulters. Eight and thirty fold increases in the larval body and silkgland weights respectively were the result of SSP-11. Further, total proteins, total carbohydrates, glycogen and nucleic acid (DNA & RNA) also increased. The activity levels of alanine aminotransferase and aspartate aminotransferasc increased significantly than in the control indicating increased mobilization of aminoacids into transamination activities.


Bombyx mori; trimoulterization; silkworm; silkgland; fat body; tissue composition; SSP-11

Full Text:



Anonymous (1994) Annual report, 1993-94, CSRTI. Mysore. India, pp. 68.

Akai, H.. K. Kimura. M. Kiuchi and A. Shibukawa. 1984. Effect of anti-juvenoid treatment on cocoon and cocoon filaments in Bombyx mori. J. Serie. Sci. Jpn. 53 : 545-546.

Akai. H.. M. Kiuchi, M. and Κ. Kimura. 1986. Effect of anti-JH treatment on the size and fine structure of the cocoon filament of Bombyx mori. J. Serie. Sci. Jpn. 55 : 388-396.

Carroll, N.V., R.W. Longley and J.H. Roe. 1956. Glycogen determination in liver and muscle bv use of enthrone reagent. J. Biol. Chem. 220 : 583-593.

Gaaboub. I.A., M.S. El Helaly and S.M. Mustafa. 1985. Food utilization rate of larval growth and fecundity of Bombyx mori L. (Lepidoptera: Bombycidac) fed with mulberry leaves treated with methoprene. triprcne and diflubenzuron. J. Econ. Entomol. 78 : 1182-1186.

Gii. S.H.. Y. [washita and H. Kawasaki. 1988. Endocrinological changes of trimoulter silkworms treated by an imidazole compound. J. Scric. Sci. Jpn. 57 : 210-215.

Kimura. K. M. Kiuchi and H. Akai. 1986. Effect of JH analogue and anti-JH treatments on the number and size of the silkworm egg. J. Serie. Sci. Jpn. 55 : 335-337.

Kiuchi. M., K. Kimura and H. Akai. 1985. Induction of trimoulters from tetramoultcr strains of Bombyx mori by anti-juvenoid treatment. J. Serie. Sei. Jpn. 54: 77-81.

Kubota, T., M. Kiuchi and H. Akai. 1988. Effect of repeated treatments of the anti-JH on dietary efficiency and size of the cocoon filament in precocious trimoulter silkworm. J. Serie. Sei. Jpn. 57 : 409-414.

Kuwano, E„ R. Takaya and M. Eto. 1983. Terpenoid imidazolcs. New anti-juvenile hormone. Agri. Biol. Chcm. 47 : 921-923.

Lowry, O.H.. N.J. Rosenbrough, A.L. Farr and R.J. Randall. 1951. Protein measurement with folin - phenol reagent. J. Biol, Chem. 220 :583-593.

Montgomery, R. 1975. Determination of glycogen. Arch. Bioch. Biophy. 67 : 378-386.

Moore. S. and W.H. Stain. 1954. A modified ninhydrin reagent for photometric determination of aminoacids and related compounds. J. Biol. Chem. 211 : 907-913.

Munro. H.N. and A. Fleck. 1956. The determination of nucleic acids. Meth. Bioch. Anal. 14 : 113-176.

Rcitman, S. and A. Frankel. 1957. A colorimetrie determination νof serum glutamic oxalo acetic and glutamic pvruvale transaminases. Amer. J. Clin. Pathol. 28 : 56- 63.

Ring. R.A. 1973. Changes in dry weight, protein and nucleic acid content during diapause and normal development of the blow Qy, Lucìlia sericola. J. Ins. Physiol. 19:481-495.

Tojo, S.. M. Nagata and S. Kimura. 1981. Hormonal control of storage protein synthesis and uptake by the fat bodv in the silkworm. Bombyx mori. J. Ins. Physiol. 27: 491-498.

Washida. S. 1984. Influence of juvenile hormone analogues applied at IV instar on larval growth and cocoon weiaht in Bombyx mori. J. Insect. Physiol. 16 : 210-215.


  • There are currently no refbacks.

Copyright (c) 2017 K. Υenkata Rami Reddy, K. Sashindran Nair, S. Β. Magadum, R. Κ. Datta