Molecular characterization of 18S rDNA partial sequence in Microcosmus (Stolidobranchiata, Pyuridae)

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Published: Dec 1, 2006
DOI: https://doi.org/10.12681/mms.165
D. FULGIONE
Dipartimento di Biologia Strutturale e Funzionale, Università di Napoli Federico II. Via Cinthia MSA, Napoli
D. RIPPA
Dipartimento delle Scienze Biologiche sezione di Zoologia. Università di Napoli Federico II. Via Mezzocannone 8 – 80134 Napoli
A. DE LUCA
Museo di Anatomia Veterinaria, Università di Napoli Federico II
M. MILONE
Abstract
We present a 18S rDNA based molecular phylogeny of two species of the genus Microcosmus (M. sulcatus and M. claudicans) sampled in the Mediterranean, to investigate their phylogenetic position relative to species of the order Stolidobranchiata. The analysis is based on partial sequences (739 bp) of the 18S rDNA. Among the 18 variable sites found between the two species, 4 correspond to transitions (ts), 14 to transversions (tv) and 4 to deletions/insertions. In the considered Stolidobranchiata, we found 4.3% overall mean number of nucleotide differences and 0.06 (S.E. ±0.01) Kimura 2-parameter distance. The mean number of nucleotide differences between Microcosmus spp. and other Stolidobranchiata species was of 6% and 0.08 (S.E. ±0.01) Kimura 2-parameter distance. A molecular phylogeny obtained by Maximum Parsimony corroborates results of the traditional taxonomy.
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