New morphological and molecular evidence confirm the presence of the Norwegian skate Dipturus nidarosiensis (Storm, 1881) in the Mediterranean Sea and extend its distribution to the western basin
Published:
Jul 17, 2017
Keywords:
Alboran Sea deep-sea skate Dipturus nidarosiensis DNA barcoding misidentification western Mediterranean
Abstract
The present study confirms the presence of the Norwegian skate Dipturus nidarosiensis (Storm, 1881) in the Mediterranean Sea, by means of morphological traits and molecular markers providing the first record of this species in the Alboran Sea. Cannas et al. (2010) reported D. nidarosiensis for the first time in the Mediterranean from specimens captured in the central western basin, but Ebert & Stehmann (2013) and Stehmann et al. (2015) considered these records "likely refer to the smaller morphotype, Dipturus sp.", a species not yet described. Eight specimens of the Dipturus genus (Rajiformes: Rajidae) were caught off the Alboran Island (western Mediterranean) in 2012, 2013 and 2016, between 620 and 819 m depth. These specimens showed morphometric diagnostic features corresponding to those of Norwegian skates from the Northeast Atlantic and the central western Mediterranean Sea. Moreover, the Alboran individuals were genetically compared to Northeast Atlantic specimens available in GenBank by means of two mitochondrial DNA fragments: cytochrome c oxidase subunit I (COI) and cytochrome b (Cytb). Analyses showed that the Northeast Atlantic Norwegian skate specimens and the Alboran Sea ones were genetically similar and shared haplotypes, corroborating the identification of the Alboran individuals as Dipturus nidarosiensis. However, they were different from other Dipturus species distributed throughout the Northeast Atlantic and the Mediterranean Sea. Our results confirm the occurrence of this deep-sea large skate species in the Mediterranean, although the IUCN Red List of Threatened Species does not consider it possible (Stehmann et al., 2015).
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RAMÍREZ-AMARO, S., ORDINES, F., ÁNGEL PUERTO, M., GARCÍA, C., RAMON, C., TERRASA, B., & MASSUTÍ, E. (2017). New morphological and molecular evidence confirm the presence of the Norwegian skate Dipturus nidarosiensis (Storm, 1881) in the Mediterranean Sea and extend its distribution to the western basin. Mediterranean Marine Science, 18(2), 251–259. https://doi.org/10.12681/mms.1950
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- Vol. 18 No. 2 (2017)
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- Research Article
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References
Baro, J., Rueda, J.L., Díaz del Río, V., 2012. South Iberian submarine canyons in the Alboran sea: geohabitats, associated communities and fisheries resources. In Mediterranean Submarine Canyons: Ecology and Governance. Würtz, M. (Ed). IUCN, Málaga, Spain, p. 145-156.
Bertrand, J.A., Gil de Sola, L., Papaconstantinou, C., Relini, G., Souplet, A., 2002. The general specifications of the MEDITS surveys. Scientia Marina, 66, 9-17.
Cannas, R., Follesa, M.C., Cabiddu, S., Porcu, C., Salvadori, S. et al., 2010. Molecular and morphological evidence of the occurrence of the Norwegian skate Dipturus nidarosiensis (Storm, 1881) in the Mediterranean Sea. Marine Biology Research, 6, 341-350.
Cariani, A., Messinetti, S., Ferrari, A., Arculeo, M., Bonello, J. et al., 2017. Improving the conservation of Mediterranean chondrichthyans: the ELASMOMED DNA barcode reference library. PLoS ONE 12 (1): e0170244.
Clement, M., Posada, D., Crandall, K., 2000. TCS: a computer program to estimate gene genealogies. Molecular Ecology, 9, 1657-1660.
Coll, M., Piroddi, C., Steenbeek, J., Kaschner, K., Rais Lasram, F.B. et al., 2010. The biodiversity of the Mediterranean Sea: estimates, patterns and threats. PLoS ONE. 5:e11842.
Darriba, D., Taboada, G.L., Doallo, R., Posada, D., 2012. jModelTest2: more models, new heuristics and parallel computing. Nature Methods. 9 (8), 772.
Danovaro, R., Company, J. B., Corinaldesi, C., D’Onghia, G., Galil, B. et al., 2010. Deep-sea biodiversity in the Mediterranean Sea: The known, the unknown, and unknowable. PLoS ONE. 5 (8): e11832.
Dulvy, N.K., Notarbartolo di Sciara, G., Serena, F., Tinti, F., Ungaro, N., Mancusi, C., Ellis, J., 2006. Dipturus batis. The IUCN Red List of Threatened Species. http://www. iucnredlist.org/details/39397/0 (accessed 10 August 2015)
Ebert, D.A., Compagno, L.J.V., 2007. Biodiversity and systematics of skates (Chondrichthyes: Rajiformes: Rajoidei). Environmental Biology of Fishes, 80, 111-124.
Ebert, D.A., Stehmann, M., 2013. Sharks, batoids, and chimaeras of the North Atlantic. FAO Species Catalogue of fishery Purpose. No. 7. Rome,523 pp.
Follesa, M.C., Cannas, R., Cabiddu, S., Cau, A., Mulas, A., Porcu, C., Cau, A., 2012. Preliminary observations of the reproductive biology and diet of the Norwegian skate Dipturus nidarosiensis (Rajidae) from Central Western Mediterranean. Cybium 36 (3), 473-477.
Griffiths A.M., Sims D.W., Cotterell S.P., Nagar A.E., Ellis J.R. et al., 2010. Molecular markers reveal spatially segregated cryptic species in a critically endangered fish, the common skate (Dipturus batis). Proceeding of the Royal Society Biological Science, 277, 1497-1503.
Hall, T.A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/ NT. Nucleic Acids Symposium Series, 41, 95-98.
Hasegawa, M., Kishino, H., Yano, T., 1985. Dating the humanape split by a molecular clock of mitochondrial DNA. Journal of Molecular Evolution, 22, 160-174.
ICES., 2015. Report of the Working Group on Elasmobranch Fishes (WGEF). International Council for the Exploration of the Sea (ICES). CM/ACOM. Lisbon, Portugal. 711 pp.
Iglésias, S.P., Toulhoat, L., Sellos, D.Y., 2010. Taxonomic confusion and market mislabelling of threatened skates: important consequences for their conservation status. Aquatic Conservation: Marine and Freshwater Ecosystems, 20, 319-333.
Ivanova, N.V., Zemlak, T.S., Hanner, R., Hebert, P.D.N., 2007. Universal primer cocktails for fish DNA barcoding. Molecular Ecology Notes, 7, 544-548.
Lynghammar, A., Christiansen, J.S., Griffiths, A.M., Fevolden, S.E., Hop, H., Bakken, T., 2014. DNA barcoding of the northern Northeast Atlantic skates (Chondrichthyes, Rajiformes), with remarks on the widely distributed starry ray. Zoological Scripta, 43(5), 485-495.
Millot, C., 1999. Circulation in the Western Mediterranean Sea. Journal of Marine Systems, 20, 423-442.
Moranta, J., Massutí, E., Palmer, M., Gordon, J.D.M., 2007. Geographic and bathymetric trends in abundance, biomass and body size of four grenadier fishes along the Iberian coast in the western Mediterranean. Progress in Oceanography, 72, 63-83.
Palumbi, S.R., 1996. Nucleic acids II: the polymerase chain reaction. p. 205-248. In: Molecular Systematics. Hillis, D.M., Moritz, C., Mable, B.K. (Eds.).
Sinauer, Sunderland MA. Patarnello, T., Volckaert, F.A.M.J., Castilho, R., 2007. Pillars of Hercules: is the Atlantic-Mediterranean transition a phylogeographical break?Molecular Ecology, 16, 4426-4444.
Ramírez-Amaro, S., Ordines, F., Terrasa, B., Esteban, A., García, C., Guijarro, B., Massutí, E., 2015. Demersal chondrichthyans in the western Mediterranean: assemblages and biological parameters of their main species. Marine and Freshwater Research, 67, 636-652.
Ratnasingham, S., Hebert, P.D.N., 2007. BOLD: The Barcode of Life Data System (www.barcodinglife.org). Molecular Ecology Notes, 7, 355-364.
Rey, J., Coelho, R., Lloris, D., Séret, B., Gil de Sola, L., 2010. Distribution patterns of Galeus atlanticus in the Alboran Sea (south western Mediterranean) and some sexual character comparison with Galeus melastomus. Marine Biology Research, 6 (4), 364-372.
Rodríguez-Cabello, C., Pérez, M., Sánchez, F., 2013. New records of chondrichthyans species caught in the Cantabrian Sea (southern Bay of Biscay). Journal of Marine Biological Association of the United Kingdom, 93 (7), 1929-19239.
Stehmann, M., 1995. First and new records of skates (Chondrichthyes, Rajiformes, Rajidae) from the West African continental slope (Morocco to South Africa), with descriptions of two new species. Archive of Fishery and Marine Research, 43 (1), 1-119.
Stehmann, M.F.W., Ellis, J., Walls, R., Lynghammar, A., 2015. Dipturus nidarosiensis. The IUCN Red List of Threatened Species. http://www.iucnredlist.org/details/161729/0 (Accessed 26 August 2016).
Stevens, J.D., Bonfil, R., Dulvy, N.K., Walker, P.A., 2000. The effects of fishing on sharks, rays, and chimaeras (Chondrichthyans), and the implications for marine ecosystems. ICES Journal of Marine Science, 57, 476-494.
Tamura, K., Stecher, G., Peterson, D., Filipski, A., Kumar, S., 2013. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution, 30, 2725- 2729.
Tamura, K., 1992. Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Molecular Biology and Evolution, 9, 678-687.
Terrasa, B., Pérez-Mellado, V., Brown, R., Picornell, A., Castro, J.A., Ramon, M., 2009. Foundations for conservation of intraspecific genetic diversity revealed by analysis of phylogeographical structure in the endangered endemic lizard Podarcis lilfordi. Diversity and Distribution, 15, 207-221.
Weigmann, S., 2016. Annotated checklist of the living sharks, batoids and chimaeras (Chondrichthyes) of the world, with a focus on biogeographical diversity. Journal of Fish Biology, 88, 837-1037.
