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Relationship between marine epilithic diatoms and environmental variables in oligotrophic bay, NE Mediterranean

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DUBRAVKA HAFNER (http://orcid.org/0000-0001-7379-4719), ANA CAR (http://orcid.org/0000-0001-6018-4305), NENAD JASPRICA, TATJANA KAPETANOVIĆ, IRIS DUPČIĆ RADIĆ


The taxonomic composition and structure of a marine epilithic diatom community were sampled from the bottom of the two sites at monthly intervals from January to December 2011 in the small semi-enclosed oligotrophic Neum Bay in Bosnia and Herzegovina (Middle Adriatic). Altogether, 264 diatom taxa (species and infraspecific taxa) within 69 genera were identified. Among them, 149 and 203 taxa occurred in samples from the shallow (0.5 m depth) and deep (8 m depth) sites, respectively. The monthly distribution of most of the diatoms was irregular and high numbers of sporadic taxa were found. SIMPER analysis indicated that the difference between shallow and deep sites could be largely attributed to the frequently recorded diatom taxa and those with high percentage abundances. They were Halamphora coffeiformis, Caloneis excentrica, Cocconeis scutellum var. scutellum, Licmophora flabellata, Licmophora gracilis, Licmophora sp., Navicula abunda, Rhabdonema adriaticum, and Striatella unipunctata. Canonical Correspondence Analysis (CCA) showed that temperature, oxygen saturation (O2/O2′), silicate concentration (SiO4), and salinity were the most important factors influencing diatom community structure in the bay.


Bacillariophyta; shallow-water habitat; environmental factors; diversity; eastern Adriatic; Bosnia and Herzegovina

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Admiraal, W., 1984. The ecology of estuarine sediment-inhabiting diatoms. p. 269-322. In: Progress in phycological research 3. Round, F.E., Chapman, G. (Eds). Biopress Ltd., Bristol.

Agatz, M., Asmus, R.M, Deventer, B., 1999. Structural changes in the benthic diatom community along a eutrophication gradient on a tidal flat. Helgoland Marine Research, 53, 92-101.

Álvarez-Blanco, I., Blanco, S., 2014. Benthic diatoms from Mediterranean coasts. Bibliotheca Diatomologica, 60, 1-409.

Beisel, J.N., Usseglio-Polatera, P., Bachmann, V., Moreteau J.C., 2003. A comparative analysis of evenness index sensitivity. International Review of Hydrobiology, 88, 3-15.

Bérard-Therriault, L., Cardinal, A., Poulin, M., 1986. Les diatomées (Bacillariophyceae) benthiques de substrats durs des eaux marines et saumâtres du Québec. 6. Naviculales: Cymbellaceae et Gomphonemaceae. Le Naturaliste Canadien, 113, 405-429.

Bérard-Therriault, L., Cardinal, A., Poulin, M., 1987. Les diatomées (Bacillariophyceae) benthiques de substrats durs des eaux marines et saumâtres du Québec. 8. Centrales. Le Naturaliste Canadien, 114, 81-113.

Brandini, F.P., da Silva, E.T., Pellizzari, F.M., Fonseca, A.L.O., Fernandes, L.F., 2001. Production and biomass accumulation of periphytic diatoms growing on glass slides during a 1-year cycle in a subtropical estuarine environment (Bay of Paranaguá, southern Brazil). Marine Biology, 138, 163-171.

Burić, Z., Caput, K., Viličić D., 2004. Distribution of the diatom Cocconeis scutellum in the karstic estuary (Zrmanja, eastern Adriatic Sea). Biologia, 59, 1-7.

Čalić, M., Carić, M., Kršinić, F., Jasprica, N., Pećarević, M., 2013. Controlling factors of phytoplankton seasonal succession in oligotrophic Mali Ston Bay (south-eastern Adriatic). Environmental Monitoring and Assessment, 185, 7543-7563.

Caput Mihalić, K., Viličić, D., Ahel, M., Burić, Z., Carić, M., 2008. Periphytic algae development in the upper reach of the Zrmanja Estuary (eastern Adriatic coast). Vie Milieu, 58, 203-213.

Car, A., Witkowski, A., Dobosz, S., Burfeind, D.D., Meinesz, A. et al., 2012. Description of a new marine diatom Cocconeis caulerpacola sp. nov. (Bacillariophyceae), epiphytic on invasive Caulerpa species. European Journal of Phycology, 47, 433-448.

Chen, C.P., Gao, Y.H., Lin, P., 2010. Geographical and seasonal patterns of epiphytic diatoms on a subtropical mangrove (Kandelia candel) in southern China. Ecological Indicators, 10, 143-147.

Cibic, T., Facca, C., 2010. Microphytobenthos. In: Relini, G. (Ed.), Checklist della flora e della fauna dei mari italiani. Parte II. Biologia Marina Mediterranea, 17 (suppl. 1), 754-800.

Clarke, K.R., Gorley, R.N., 2001. PRIMER v5: User Manual/Tutorial. PRIMER-E, Plymouth, 91 pp.

Clarke, K.R., Warwick, R.M., 1994. Change in marine communities: an approach to statistical analysis and interpretation. Natural Environmental Research Council, Plymouth Marine Laboratory, Plymouth, 144 pp.

Clarke K.R., Warwick, R.M., 2001. Change in marine communities: an approach to statistical analysis and interpretation. 2nd ed., PRIMER-E, Ltd., Plymouth Marine Laboratory, Plymouth.

Çolak Sabanci, F., 2011. Relationship of epilithic diatom communities to environmental variables in Homa lagoon (Izmir, Turkey). Aquatic Biology, 13, 233-241.

Çolak Sabanci, F., 2012. Taxonomic survey of benthic diatoms on natural substrata from coastal lagoon (Aegean Sea, Turkey). Turkish Journal of Fisheries and Aquatic Sciences, 12, 841-849.

Çolak Sabanci, F., 2013. Species of Mastogloia (Bacillariophyceae) - new for the Aegean coast of Turkey. Mediterranean Marine Science, 14, 129-140.

Çolak Sabanci F., Koray, T., 2010. Four new records for the benthic diatoms (genera Cocconeis, Seminavis, Synedra and Trachysphenia) from the Aegean Sea. Turkish Journal of Botany, 34, 531-540.

Çolak Sabanci, F., Sapanci, M., Koray, T., Buyukisik, B., 2011. A qualitative study of the microphytobenthic communities of Homa Lagoon (Izmir-Turkey). Fresenius Environmental Bulletin, 20, 346-353.

De Stefano, M., Marino, D., Mazzella, L., 2000. Marine taxa of Cocconeis on leaves of Posidonia oceanica, including a new species and two new varieties. European Journal of Phycology, 35, 225-242.

De Stefano, M., Romero, O.E., Totti, C., 2008. A comparative study of Cocconeis scutellum Ehrenberg and its varieties (Bacillariophyta). Botanica Marina, 51, 506-536.

Delgado, C., Pardo, I., García, L., 2010. A multimetric diatom index to assess the ecological status of coastal Galician rivers (NW Spain). Hydrobiologia, 644, 371-384.

Dupčić Radić, I., Hrustić, E., Jasprica, N., 2013. Koncentracija hranjivih soli i klorofila a u Neumskom zaljevu (Bosna i Hercegovina). p. 55. In: Abstracts of the 4th Croatian Botanical Symposium with international participation, Split 2013. Alegro, A., Boršić, I. (Eds). Croatian Botanical Society, Split.

Facca, C., Sfriso, A., Socal, G., 2002. Temporal and spatial distribution of diatoms in the surface sediments of the Venice Lagoon. Botanica Marina, 45, 170-183.

Facca, C., Sfriso, A., 2007. Epipelic diatom spatial and temporal distribution and relationship with the main environmental parameters in coastal waters. Estuarine, Coastal and Shelf Science, 75, 35-49.

Falkowski, P.G., Schofield, O., Katz, M.E., Van de Schootbrugge, B., Knoll, A.H., 2004. Why is the land green and the ocean red? In: Coccolithophores. From molecular processes to global impacy. Thierstein, H.R., Young, J.R. (Eds). Springer, Berlin, 429-453.

Guiry, M.D., Guiry, G.M., 2017. AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. Available at: http://www.algaebase.org; (Accessed 9 November 2017).

Hartley, B., 1986. A check-list of the freshwater, brackish and marine diatoms of the British Isles and adjoining coastal waters. Journal of the Marine Biological Association of the United Kingdom, 66, 531-610.

Hartley, B., Barber, H.G., Carter, J.R., Sims, P.A., 1996. An Atlas of British Diatoms. Biopress Ltd., Bristol, 601 pp.

Hasle, G.R., Fryxell, G.A., 1970. Diatoms: cleaning and mounting for light and electron microscopy. Transactions of the American Microscopical Society, 84, 469-474.

Hendey, N.I., 1964. An introductory account of the smaller algae of British coastal waters. Part V. Bacillariophyceae (Diatoms). Ministry of Agriculture, Fisheries and Food, Fisheries Investigations, Seies IV, London, 317 pp.

Hillebrand, H., Sommer, U., 2000. Diversity of benthic microalgae in response to colonization time and eutrophication. Aquatic Botany, 67, 221-236.

Hillebrand, H., Worm, B., Lotze, H.K., 2000. Marine microbenthic community structure regulated by nitrogen loading and grazing pressure. Marine Ecology Progress Series, 204, 27-38.

Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W., Strickland, J.D.H., 1965. Fluorometric determination of chlorophyll. Journal Du Conseil, 301, 3-15.

Ivančić, I., Degobbis, D., 1984. An optimal manual procedure for ammonia analysis in natural waters by the indophenol blue method. Water Research, 18, 1143-1147.

Jasprica, N., Carić, M., Kršinić, F., Kapetanović, T., Batistić, M., Njire, J., 2012. Planktonic diatoms and their environment in the lower Neretva River estuary (Eastern Adriatic Sea, NE Mediterranean). Nova Hedwigia, 141, 405-430.

Jasprica, N., Dolina, K., Milović, M., 2015. Plant taxa and communities on three islets in south Croatia, NE Mediterranean. Natura Croatica, 24, 191-213.

Jasprica, N., Hafner, D., 2005. Taxonomic composition and seasonality of diatoms in three Dinaric karstic lakes in Croatia. Limnologica, 35, 304-319.

Jasprica, N., Hafner, D., Batistić, M., Kapetanović, T., 2005. Phytoplankton in three freshwater lakes in the Neretva River delta (Eastern Adriatic, NE Mediterranean). Nova Hedwigia, 81, 37-54.

Karydis, M., 2009.Eutrophication assessment of coastal waters based on indicators: a literature review. Global NEST Journal, 11, 373-390.

Korte, V.L., Blinn, D.W., 1983. Diatom colonization on artificial substrata in pool and riffle zones studied by light and scanning electron microscopy. Journal of Phycology, 19, 332-341.

Krammer, K., Lange-Bertalot, H., 1991. Bacillariophyceae: Achnanthaceae, Kritische Ergänzungen zu Navicula (Lineolatae) und Gomphonema. Gesamtliteraturverzeichnis. p. 1-437. In: Süβwasserflora von Mitteleuropa. Ettl, H., Gerloff, J., Heynig, H., Mollenhauer, D. (Eds). Vol. 2/4. Stuttgart: Gustav Fischer Verlag.

Krebs, C.J., 1999. Ecological methodology. 2nd ed., Addison Wesley Longman, Menlo Park, 620 pp.

Kwandrans, J., 2007. Diversity and ecology of benthic diatom communities in relation to acidity, acidification and recovery of lakes and rivers. Diatom Monographs (v.9). Koeltz Scientific Books, Königstein, 168 pp.

Lavoie, I., Campeau, S., Zugic-Drakulic, N., Winterand, J.G., Fortin, C., 2014. Using diatoms to monitor stream biological integrity in Eastern Canada: an overview of 10 years of index development and ongoing challenges. Science of the Total Environment, 475, 187-200.

Legendre, L., Legendre, P., 1983. Numerical ecology. Developments in environmental modelling. 3. Elsevier, Amsterdam, 419 pp.

Levkov, Z., Caput Mihalić, K., Ector, L., 2010. A taxonomical study of Rhoicosphenia Grunow (Bacillariophyceae) with a key for identification of selected taxa. Fottea, 10, 145-200.

MacLulich, J. H., 1987. Variations in the density and variety of intertidal epilithic microflora. Marine Ecology Progress Series, 40, 285-293.

Margalef, R., 1969. Size of centric diatoms as an ecological indicator. Mitteilung Internationale Vereinigung fuer Theoretische unde Amgewandte Limnologie, 17, 202-210.

Martinez-Goss, M.R., Evangelista, L.T., 2011. A contribution to the taxonomy of Mastogloia (Class Bacillariophyceae) in the Philippines. Philippine Journal of Science, 140, 7-12.

McQuoid, M.R., Nordberg, K., 2003. The diatom Paralia sulcata as an environmental indicator species in coastal sediments. Estuarine, Coastal and Shelf Science, 56, 339-354.

McIntyre, C.D., Moore, W.M., 1977. Marine littoral diatoms: Ecological consideration. In: The biology of diatoms. Werner, D. (Ed). Blackwell Scientific Publications, Oxford, 333-371.

Mejdandžić, M., Ivanković, T., Pfannkuchen, M., Godrijan, J., Marić Pfannkuchen, D., Hrenović, J., Ljubešić, Z., 2015. Colonization of diatoms and bacteria on artificial substrates in the northeastern coastal Adriatic Sea. Acta Botanica Croatica, 74, 407-422.

Miho, A., Witkowski, A., 2005. Diatom (Bacillariophyta) flora of Albanian coastal wetlands taxonomy and ecology: A review. Proceedings of the California Academy of Sciences, 56, 129-145.

Müller, U., 1999. The vertical zonation of adpressed diatoms and other epiphytic algae on Phragmites australis. European Journal of Phycology, 34, 487-496.

Munda, I. M., 2005. Seasonal fouling by diatoms on artificial substrata at different depths near Piran (Gulf of Trieste, Northern Adriatic). Acta Adriatica, 46, 137-157.

Nenadović, T., Šarčević, T., Čižmek, H., Godrijan, J., Marić Pfannkuchen, D. et al., 2015. Development of periphytic diatoms on different artificial substrates in the Eastern Adriatic Sea. Acta Botanica Croatica, 74, 377-392.

Pandžić, K., Likso, T., 2013. Climate monitoring and assessment for 2011. Reviews no. 23. Croatian Meteorological and Hydrological Service, Zagreb, 33 pp.

Pennesi, C., Poulin, M., De Stefano, M., Romagnoli, T., Totti, C., 2011. New insights to the ultrastructure of some marine Mastogloia species section Sulcatae (Bacillariophyceae), including Mastogloia neoborneensis sp. nov. Phycologia, 50, 548-562.

Peragallo, H., Peragallo, M., 1897−1908. Diatomées marines de France et des districts maritimes voisin. Micrographe-Editeur, Grez-sur-Loing (S.-et-M.), 491 pp.

Pielou, E. C., 1966. The measurement of diversity in different types of biological collections. Journal of Theoretical Biology, 13, 131-144.

Pikelj, K., Juračić, M., 2013. Eastern Adriatic coast (EAC): Geomorphology and coastal vulnerability of a karstic coast. Journal of Coastal Research, 29, 944-957.

Poulin, M., Bérard-Therriault, L., Cardinal, A., 1984. Les diatomées benthiques de substrats durs des eaux marines et saumâtres du Québec. 3. Fragilarioideae (Fragilariales, Fragilariaceae). Le Naturaliste Canadien, 111, 349-367.

Poulin, M., Bérard-Therriault, L., Cardinal, A., Hamilton, P.B., 1990. Les diatomées (Bacillariophyta) benthiques de substrats durs des eaux marines et saumâtres du Québec. 9. Bacillariaceae. Le Naturaliste Canadien, 117, 73-101.

Ricard, M., 1974. Etude taxonomique des diatomées marines du lagon de Vairao (Tahiti) 1. Le genre Mastogloia. Revue Algologique, nouvelle série, 11, 161-177.

Ricard, M., 1975. Quelques diatomées nouvelles de Tahiti décrites en microscopie photonique et électronique à balayage. Bulletin du Musée National d’Histoire Naturelle, 3e serie, 326, 201-229.

Ricard, M., 1977. Les peuplements de diatomeés des lagons de l’Archipel de la Société (Polynésie Française). Revue Algologique, nouvelle série, 12, 137-336.

Romagnoli, T., Bavestrello, G., Cucchiari, E.M., De Stefano, M., Di Camillo, C. et al., 2007. Microalgal communities epibiontic on the marine hydroid Eudendrium racemosum in the Ligurian Sea during an annual cycle. Marine Biology, 151, 537-552.

Romagnoli, T., Totti, C., Accoroni, S., De Stefano, M., Pennesi, C., 2014. SEM analysis of the epibenthic diatoms on Eudendrium racemosum (Hydrozoa) from the Mediterranean Sea. Turkish Journal of Botany, 38, 566-594.

Round, F.E., Sloane, J.F., Ebling, F.J., Kitching, J.A., 1961. The ecology of Lough Ine: X. The hydroid Sertularia operculata (L.) and its associated flora and fauna: ejects of transference to sheltered waters. Journal of Ecology, 49, 617-629.

Sagan, G., Radureau, A., Le Rouzic, B., Feunteun, E., 2000. Spatio-temporal variations of microphytobenthos community structure and biomass in macrotidal salt marshes of the Mont Saint-Michel Bay. Task 5. In: European salt marsh modelling. Feunteun, E., Thorin, S., Lefeuvre, J.-C. (Eds). EU programme Environment and Climate. ENV4-CT97-0436. University of Rennes 1, 172-207.

Siqueiros-Beltrones, D.A., Ibarra-Obando, S.E., Loya-Salinas, D.H., 1985. An approximation to the floristic structure of the epiphytic diatoms of Zostera marina and its temporal variations in Bahía Falsa, San Quintín, B.C. Ciencias Marinas, 11, 69-88.

Smith, B., Wilson, J.B., 1996. A consumer’s guide to evenness indices. Oikos, 76, 70-82.

Snoeijs, P., 1993. Intercalibration and distribution of diatoms in the Baltic Sea, 1: 1–129. Baltic Marine Biologists Publication 16a, Opulus Press, Uppsala.

Snoeijs, P., 1999. Marine and brackish waters. In: Swedish Plant Geography. Snoeijs, P., Diekmann, M., (Eds). Acta Phytogeographica Suecica, 94, 187-212. Opulus Press, Uppsala.

Snoeijs, P., Balashlova, N., 1998. Intercalibration and distribution of diatoms in the Baltic Sea, 5, 1-144. Baltic Marine Biologists Publication 16e, Opulus Press, Uppsala.

Snoeijs, P., Kasperoviciené, J., 1996. Intercalibration and distribution of diatoms in the Baltic Sea, 4, 1-125. Baltic Marine Biologists Publication 16d, Opulus Press, Uppsala.

Snoeijs, P., Potapova, M., 1995. Intercalibration and distribution of diatoms in the Baltic Sea, 3, 1-125. Baltic Marine Biologists Publication 16c, Opulus Press, Uppsala.

Stevenson, R. J., Pan, Y., 1999. Assessing environmental conditions in rivers and streams with diatoms. In: The Diatoms. Application for the environmental and earth sciences. Stoermer, E.F., Smol, J.P. (Eds). Cambridge University Press, Cambridge, 469 pp.

Strickland, J.D.H., Parsons, T.R., 1972. A practical handbook of seawater analysis. Journal of the Fisheries Research Board of Canada, 167, 1-310.

Sullivan, M.J., 1978. Diatom community structure: taxonomic and statistical analyses of a Mississippi salt marsh. Journal of Phycology, 14, 468-475.

Sullivan, M.J., 1984. Community structure of epiphytic diatoms from the Gulf Coast of Florida, U.S.A. In: Proceedings of 7th Diatom Symposium, Philadelphia, 1982. Mann, D.G. (Ed). O. Koeltz, Koenigstein, 373-384.

Sullivan, M.J., Currin, C.A., 2000. Community structure and functional dynamics of benthic microalgae in salt marshes. In: Concepts and controversies in tidal marsh ecology. Weinstein, M.P., Kreeger, D.A. (Eds). Kluwer Academic Publishers, Dordrecht, 81-106.

Tan, X., Ma, P., Bunn, S.E., Zhang, Q., 2015. Development of a benthic diatom index of biotic integrity (BD-IBI) for ecosystem health assessment of human dominant subtropical rivers, China. Journal of Environmental Management, 151, 286-294.

ter Braak, C.J.F., Verdonschot, P.F.M., 1995. Canonical correspondence analysis and related multivariate methods in aquatic ecology. Aquatic Sciences, 57, 255-289.

ter Braak, C.J.F., Šmilauer, P., 2002. CANOCO reference Manual and CanoDraw for Windows user’s guide: Software for Canonical Community Ordination (version 4.5). Microcomputer Power, Ithaca, New York.

Tilman, D., Kiesling, K., Sterner, R., Kilham, S.S., Johnson, F.A., 1986. Green, bluegreen and diatom algae: Taxonomic differences in competitive ability for phosphorus, silicon and nitrogen. Archiv für Hydrobiologie, 106, 473-485.

Totti, C., Cucchiari, E., De Stefano, M., Pennesi, C., Romagnoli, T. et al., 2007. Seasonal variations of epilithic diatoms on different hard substrates, in the northern Adriatic Sea. Journal of the Marine Biological Association of the United Kingdom, 87, 649-658.

Totti, C., Poulin, M., Romagnoli, T., Perrone, C., Pennesi, C. et al., 2009. Epiphytic diatom communities on intertidal seaweeds from Iceland. Polar Biology, 32, 1681-1691.

Ulanova, A., Snoeijs, P., 2006. Gradient responses of epilithic diatom communities in the Baltic Sea proper. Estuarine, Coastal and Shelf Science, 68, 661-674.

UNESCO, 1973. International oceanographic tables, Volume 2. National Institute of Oceanography of Great Britain and UNESCO, Paris.

Vollenweider, R.A., Giovanardi, F., Montanari, G., Rinaldi, A., 1998. Characterization of the trophic conditions of marine coastal waters with special reference to the NW Adriatic Sea: proposal for a trophic scale, turbidity and generalized water quality index. Environmetrics, 9, 329-357.

Weckström, K., Juggins, S., 2005. Coastal diatom-environment relationships from the Gulf of Finland, Baltic Sea. Journal of Phycology, 42, 21-35.

Weiss, R.F., 1970. The solubility of nitrogen, oxygen and argon in water and seawater. Deep Sea Research, 17, 721-735.

Wilkinson, L., 1986. Systat: The system for statistics.Systat, Inc., Evanston.

Winter, J.G., Duthie, H.C., 2000. Stream epilithic, epipelic and epiphytic diatoms: habitat fidelity and use in biomonitoring. Aquatic Ecology, 34, 345-353.

Witkowski, A., Lange-Bertalot, H., Metzelin, D., 2000. Diatom flora of marine coasts I. In: Iconographia diatomologica, vol. 7. Lange-Bertalot, H. (Ed). A. R. G. Gantner, Ruggell, Liechtenstein. 1-925.

Wuchter, C., Marquardt, J., Krumbein, W.E., 2003. The epizoic diatom community on four bryozoan species from Helgoland (German Bight, North Sea). Helgoland Marine Research, 57, 13-19.

Zong, Y., 1997. Implications of Paralia sulcata abundance in Scottish isolation basins. Diatom Research, 12, 125-150.


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