Potential crypticity within two decapod (Crustacea) genera: Galathea Fabricius, 1793 and Eualus Thallwitz, 1891 suggested by integrative taxonomic approach

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Published: Jun 20, 2022
DOI: https://doi.org/10.12681/mms.27733
Keywords:
Integrative taxonomy barcoding mitochondrial marker Mediterranean Sea.
SHEENA CONFORTI
ETH Zurich
https://orcid.org/0000-0002-0173-6170
FEDERICA COSTANTINI
Dipartimento di Scienze Biologiche, Geologiche e Ambientali & Centro Interdipartimentale di Ricerca per le Scienze Ambientali in Ravenna Università di Bologna, Campus di Ravenna, Ravenna, Italy
Abstract

Correct species identification and description are fundamental to understand the health status of marine ecosystems. The use of a single identification tool for species distinction may lead to species misidentifications, having major consequences for ecological studies. In the present research, we used an integrative taxonomic approach to identify benthic decapods belonging to the genera of Galathea Fabricius, 1793 and Eualus Thallwitz, 1891, collected in the Mediterranean Sea. 23 Galathea and 22 Eualus individuals were morphologically analyzed and sequenced at the mitochondrial COI gene to confirm their identity using the BOLD Identification Engine. Morphological identification revealed the presence of two Galathea and three Eualus species, while species delimitation based on DNA barcoding of COI sequences strongly suggested the presence of three Galathea and four Eualus species. Molecular analyses suggested the potential presence of two still undescribed species: one cryptic to Galathea squamifera and one cryptic to Galathea intermedia. Contrasting results obtained via morphological identification and the BOLD Identification Engine impeded the recognition of Eualus specimens and suggested misidentifications among BOLD reference records of Eualus cranchii, Eualus occultus, and Eualus pusiolus. These results demonstrated that morphological identification overlooks cryptic species and that misidentifications may occur, highlighting the importance of using an integrative approach to increase the current taxonomic knowledge of benthic invertebrates.

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Author Biography
SHEENA CONFORTI, ETH Zurich
Department of Environmental Microbiology, Eawag (Dübendorf, Switzerland) - PhD Candidate
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