| More

Swine artificial insemination: development and biotechnology applications

Views: 533 Downloads: 242
IA TSAKMAKIDIS, ED TZIKA, AG LYMBEROPOULOS
IA TSAKMAKIDIS, ED TZIKA, AG LYMBEROPOULOS

Abstract


Traditional artificial insemination has been highly contributed in swine global industry development. However, in order to improve the productivity and the reproductive performance of pig farms, new techniques have been performed. Among them, post cervical or intrauterine insemination, deep intrauterine insemination (DUI), intra-oviductal insemination (ΙΟΙ) by laparotomy or laparoscopy, as well as the biotechnological applications, such as sex sorted semen, frozen semen and sperm mediated genes' transfer, have been investigated concerning their efficiency in pig production development. One of the main targets of the aforementioned techniques is to benefit the potential advantages of the high genetic value boars by using the minimal number of spermatozoa needed to achieve a high fertilization rate following artificial insemination. On the other hand, biotechnology could lead in rapid transfer of high genetic potential in inaccessible areas through frozen semen. Moreover, biotechnology could support pig industry increasing the female pigs' production according to the consumers' choices and animals' welfare by sex sorted semen application, as well as producing of transgenic pigs aiming an increased disease resistance, improvement carcass composition, increased feed intake and growth rate. In vivo studies have evaluated the efficiency of the new developments in insemination technology. The results of these studies demonstrated that a satisfactory conception rate and litter size can be obtained by the insemination of low number of spermatozoa in the appropriate position of the female genital tract at the right time, close to ovulation. High farrowing rate and number of live born piglets were noticed after the post cervical insemination with low number of spermatozoa (lxlO9), compared to the traditional artificial insemination. Similar results were observed in other experiments, where 150xl06 or 3xl05 spermatozoa were inseminated using DUI or ΙΟΙtechnique, respectively. Furthermore, the improvement of the materials of the new artificial insemination procedures (catheters, extenders etc) makes the application of it simpler and easier for the workers after a few training. Although the traditional artificial insemination is an indispensable method for the commercial pig farms, the application of currently available sperm technologies is feasible to be performed in selected animals of pig farms with high quality control and management system. The aspects of the development on swine artificial insemination technology concerning the biotechnological applications of boar sperm are reported in the present study.


Keywords


Biotechnology; swine; pig; artificial insemination

Full Text:

PDF

References


Alm Κ, Peltoniemi OAT, Koskinen E, Andersson M (2006). Porcine field fertility with two different insemination doses and the effect of sperm morphology. Reprod Dom Anim, 41: 210-213.

Bolarin A, Roca J, Rodriguez-Martinez H, Hernandez M, Vazquez JM, Martinez EA. (2006). Dissimilarities in sows' ovarian status at the insemination time could explain differences in fertility between farms when frozen-thawed semen is used. Theriogenology, 65:669-680.

Chatterjee S, Gagnon C (2001). Production of reactive oxygen species by spermatozoa undergoing cooling, freezing and thawing. Mol Reprod Dev, 59: 451-458.

Day BN, Mathias Κ, Didion ΒΑ, Martinez EA, Caamano JN (2003). Deep intrauterine insemination in sows: first field trial in USA commercial farm with newly developed device. Theriogenology 59:213.

FAO Database (2002). http ://apps.f ao .org/cgi-bin/nph- db .pi? subset=agriculture

Fraser L, Strzezek J (2005). Effects of freezing-thawing on DNAintegrity of boar spermatozoa assessed by the neutral comet assay. Reprod Dornest Anim, 40:530-536.

Gerrits RJ, Lunney JK, Johnson LA, Pursei VG, Kraeling RR, Rohrer GA, Dobrinsky JR (2005). Perspectives for artificial insemination and genomics to improve global swine populations. Theriogenology, 63:283-299.

Guthrie HD, Welch GR (2005). Impact of storage prior to cryoin vitro επεξεργασία (Sperm Mediated Gene Transfer, SMGT). preservation on plasma membrane function and fertility of boar sperm. Theriogenology, 63: 396-410.

Hancock JL (1957). The fertility of natural and of artificial matings in the pig. Proc Soc Study Fertil, 9:146-158.

Hernandez M, Roca J, Gil MA, Vazquez JM, Martinez E A (2007). Adjustments on the cryopreservation conditions reduce the incidence of boar ejaculates with poor sperm freezability. Theriogenology, 67:1436-1445.

Johnson LA, Rath D, Vazquez JM, Maxwell WM, Dobrinsky JR (2005). Preselection of sex of offspring in swine for production: current status of the process and its application. Theriogenology, 63:615-624.

Johnson LA (1991). Sex preselection in swine: altered sex ratios in offspring following surgical insemination of flow sorted X- and Ybearing sperm. Reprod Dom Anim, 26: 309-314.

Κυριακής Σ, Ματζαρλής Ν. (1980) Εφαρμογή της τεχνητής σπερματεγχΰσεως με κατεψυγμένο σπέρμα στους χοίρους. Χοιροτροφικά Νέα, 6:16-18.

Lavitrano Μ, Forni Μ, Bacci ML, Di Stefano C, Varzi V, Wang H, Seren E. (2003). Sperm mediated gene transfer in pig: Selection of donor boars and optimization of DNA uptake. Mol Reprod Dev, 64:284-291.

Martinez EA, Vazquez JM, Roca J, Cuello C, Gil MA, Parrilla I, Vazquez JL, Day BN. (2005). An update on reproductive technologies with potential short-term application in pig production. Reprod Dornest Anim, 40: 300-309.

Martinez EA, Vazquez JM, Roca J, Lucas X, Gil MA, Parrilla I, Vazquez JL, Day BN. (2002). Minimum number of spermatozoa required for normal fertility after deep intrauterine insemination

in non-sedated sows. Reproduction, 123:163-170.

Martinez EA, Vazquez JM, Roca J, Lucas X, Gil MA, Parrilla I,Vazquez JL, Day BN. (2001). Successful non-surgical deep intrauterine insemination with small numbers of spermatozoa in sows. Reproduction, 122:289-296.

Matthijs A, Engel Β, Woelders Η (2003). Neutrophil recruitment and phagocytosis of boar spermatozoa after artificial insemination of sows and the effects of inseminate volume, sperm dose and specific additives in the extender. Reproduction, 125:357-367.

Roberts PK, Bilkei G (2005). Field experiences on post-cervical artificial insemination in the sow. Reprod Dornest Anim, 40:489-491.

Robertson SA (2007). Seminal fluid signaling in the female reproductive tract: lessons from rodents and pigs. J Anim Sci, 85(13 Suppl): E36-44.

Rozeboom KJ, Reicks DL, Wilson ME (2004). The reproductive performance and factors affecting on-farm application of low-dose intrauterine deposit of semen in sows. J Anim Sci. 82: 2164-2168.

Tummaruk P, Sumransap P, Techakumphu M, Kunavongkrit A (2007). Distribution of spermatozoa and embryos in the female reproductive tract after unilateral deep intra uterine insemination in the pig. Reprod Dornest Anim 42:603-609.

Vazquez JM, Martinez EA, Roca J, Gil MA, Parrilla I, Cuello C, Carvajal G, Lucas X, Vazquez JL (2005a). Improving the efficiency of sperm technologies in pigs: the value of deep intrauterine

insemination. Theriogenology, 63:536-547.

Vazquez JM, Martinez EA, Parrilla I, Cuello C, Gil MA, Garcia E et al. (2005b). Laparoscopic intraoviductal insemination with boar spermatozoa. Reprod Dornest Anim, 40:375.

Vazquez JM, Martinez EA, Parrilla I, Roca J, Gil MA, Vazquez JL (2003). Birth of piglets after deep intrauterine insemination with flow cytometrically sorted boar spermatozoa. Theriogenology,

:1605-1614.

Vazquez JL, Martinez EA, Vazquez JM, Lucas X, Gil MA, Parrilla I, Roca J (1999). Development of a non-surgical deep intrauterine insemination technique. In: Proceedings of the IV International

Conference on Boar Semen Preservation, p: 35.

Wagner HG, Thibier M (2000). Word statistics for artificial insemination in small ruminants and swine. 14th International Congress on Animal Reproduction. Abstract 15:3, Stockholm, Sweden. Watson PF, Behan JR (2002). Intrauterine insemination of sows with reduced sperm numbers: results of a commercially based field trial. Theriogenology, 57:1683-1693.

Watson PF (2000). The causes of reduced fertility with cryopreserved semen. Anim Reprod Sci, 60-61:481-492.

Xu X, Pommier S, Arbov T, Hutchings B, Sotto W, Foxcroft GR (1998): In vitro maturation and fertilization techniques for assessment of semen quality and boar fertility. J Anim Sci, 76:3079-3089.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 IA TSAKMAKIDIS, ED TZIKA, AG LYMBEROPOULOS

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.