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Marteiliosis in molluscs

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The protozoans, belonging to the phylum Paramyxea and particularly to the genera Marteilia, are the more important mollusc pathogens. The last thirty years marteiliosis has caused economic losses in mollusc aquaculture worldwide. According to the World Organisation for Animal Health (O.I.E.), it is not a zoonosis and it is classified in List II that includes mollusc diseases which are an obligatory statement in the European Union. The disease is characterized by progressive loss of body-weight of the infected shellfish and alteration of the colour of the digestive gland. Because of the absence of pathognomonic symptoms, histological and molecular techniques are required for the diagnosis of the disease. In Greece, marteiliosis constitutes a continuous threat for the mollusc aquaculture and demands surveillance and proper management. Genetic improvement of host species, in order to produce individuals resistant to the disease, as well as knowledge of the biology of Marteilia sp. constitute useful tools for the reduction of the impact of marteiliosis.


Marteilia sp; Mytilus galloprovincialis; Ostrea edulis

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Adlard RD, Ernst I (1995) Extended range of the oyster pathogen Martellici sydneyi. Bull Eur Assoc Fish Pathol, 15: 119-121.

Alderman DJ (1979) Epizootiology of Martellici refringens in Europe. Mar. Fish. Rev, 41 (1/2): 67-69.

Anderson TJ, Adlard RD, Lester RJG (1995) Molecular diagnosis of Marteilia sydneyi (Paramyxea) in Sydney rock oysters, Saccostrea commercialis (Angas). J Fish Dis, 18: 507- 510.

Angelidis P, Virvilis C, Photis G, Chollet B, Berthe F (2001) First report of Marteilia disease of the flat oyster Ostrea edulis, in the gulf of Thessaloniki, Greece. 10th International Conference on 'Diseases of Fish and Shellfish' EAFP, 10-14 September, Dublin, Ireland.

Audemard C, Barnaud A, Collins CM, Le Roux F, Sauriau PG, Coustau C, Blachier P, Berthe FCJ (2001) Claire ponds as an experimental model for Marteilia refringens life-cycle studies: new perspectives. J Exp Mar Biol Ecol, 257: 87-108.

Audemard C, Le Roux F, Barnaud A, Collins C, Sautour Β, Sauriau PG, De Montaudouin X, Coustau C, Combes C, Berthe FCJ (2002) Needle in a haystack: involvement of the copepod Paracartia grani in the life-cycle of the oyster pathogen Marteilia refringens. Parasitology, 124: 315-323.

Audemard C, Sajus MC, Barnaud A, Sautour Β, Sauriau PG, Berthe FJC (2004) Infection dynamics of Marteilia refringens in flat oyster Ostrea edulis and copepod Paracartia grani in a Claire pond of Marennes-Oléron Bay. Dis Aquat Org, 61: 103-111.

Auffret M, Poder M (1985) Recherches sur Marteilia maurini, parasite de Mytilus edulis sur les côtes de Bretagne nord. Rev Trav Inst Pêches Mar, 47: 105-109.

Auffret M, Poder M (1987) Pathology of the main bivalve mollusc species from oyster rearing areas in Brittany (France). Aquaculture, 67: 255-257.

Balouet G (1979) Marteilia refringens - Considerations of the life-cycle and development of Aber disease in Ostrea edulis. Mar Fish Rev, 41 (1/2): 64-66.

Berthe CJF (2000) Mollusc health management: weapons for responsible aquaculture in the Mediterranean region. World Aquaculture 2000. 2-6 mai 2000. Nice. France.

Berthe CJF, Burreson E, Hine M (1999) Use of molecular tools for mollusc disease diagnosis. Bull Eur Ass Fish Pathol, 19(6): 277-278!

Berthe FCJ, Le Roux F, Adlard RD, Figueras A (2004) Marteiliosis in molluscs: A review. Aquat Living Resour, 17: 433-448.

Berthe CJF, Le Roux F, Peyretaillade E, Peyret Ρ, Rodriguez D, Gouy M, Vivares CP (2000) Phylogenetic analysis of the small subunit ribosomal RNA of Marteilia refringens validates the existence of phylum Paramyxea (Desportes and Perkins, 1990). J Eukaryotic Microbiol, 47: 288- 293.

Berthe CJF, Pernas M, Zerabib M, Haffner P, Thebault A, Figueras AJ (1998) Experimental transmission of Marteilia refringens with special consideration of its life cycle. Dis Aquat Org, 34:135-144.

Bougrier S, Tige G, Bachère E, Grizel H (1986) Ostrea angasi acclimatization to French coasts. Aquaculture, 58: 151-154.

Cahour A (1979) Marteilia refringens and Crassostrea gigas. Mar Fish Rev, 41 (1/2): 45-53.

Ceschia G, Zanchetta S, Sello M, Montesi F, Figueras A (2001) Presenza di parassiti in cannolicchi (Ensis minor e Ensis siliqua) pescati nell'area costiera del Mar Tirreno meridionale e del Mar Adriatico. Bol Soc Ital Patol Ittica, 13: 20-27.

Cunningham CO (2002) Molecular diagnosis of fish and shellfish diseases: present status and potential use in disease control. Aquaculture, 206: 19-55.

Comps M (1970) Observations sur les causes d'une mortalité anormale des huîtres plates (Ostrea edulis L.) dans le bassin de Marennes. Rev Trav Inst Pêches Mar, 34: 317-326.

Comps M (1976) Marteilia lengehi n. sp., parasite de l'huître Crassostrea cuculiata Born. Rev Trav Inst Pêches Mar, 40: 347-349.

Comps M (1985) Étude morphologique de Marteilia christenseni sp. n. parasite du lavignon Scrobicularia piperata P. (mollusque pélécypode). Rev Trav Inst Pêches Mar, 47: 99-104.

Comps M, Grizel H, Tige G, Duthoit JL (1975) Parasites nouveaux de la glande digestive des mollusques marins Mytilus edulis L. et Cardium edule. CR. Acad. Sci. Paris Sér. D. 281: 179-181.

Comps M, Pichot Y, Papayianni Ρ (1982) Recherche sur Marteilia maurini n. sp. parasite de la moule Mytilus galloprovincialis Lmk. Rev Trav Inst Pêches Mar, 45: 211-214.

Desportes I, Perkins FO (1990) Phylum Paramyxea. In: L.Margulis, J.O. Corliss, M. Melkonian, D.J. Chapman (Eds.), Handbook of Protoctista. Jones and Bartlett Publishing, Boston, 30-35.

FAO, 2003. http://faostat.fao.org.

Figueras AJ (1991) Mortalities, parasites and diseases of mussels (Mytilus edulis and Mytilus galloprovincialis) from natural and cultured populations. Bull Aquacul Assoc Canada, 91-2: 54-62.

Figueras AJ, Jardon CF, Caldas JR (1991a) Diseases and parasites of mussels (Mytilus edulis, Linnaeus, 1758) from two sites on the east coast of the United States. J Shellfish Res, 10: 89-94.

Figueras AJ, Jardon CF, Caldas JR (1991b) Diseases and parasites of rafted mussels (Mytilus galloprovincialis Lmk): preliminary results. Aquaculture, 99: 17-33.

Figueras AJ, Montes J (1988) Aber disease of edible oysters caused by Marteilia refringens. In: Fisher W.S. (Ed.). Disease Processes in Marine Bivalve Molluscs, Am Fish Soc Spec Pubi, 38-46.

Figueras AJ, Robledo JAF, Novoa B, (1996) Brown ring disease and parasites in clams (Ruditapes decussatus and R. philippinarum) from Spain and Portugal. J Shellfish Res, 15:


Fuentes J, Molares J, Villalba A (1998) Growth, mortality and parasitization of mussels cultivated in the Ria de Arousa NW Spain from two sources of seed: intertidal rocky shore vs.

collector ropes. Aquaculture, 162: 231-240.

Fuentes J, Viallalba A, Zapata C, Alvarez G (1995) Effects of stock and culture environment on infections by Marteilia refrìngens and Mytilicola intestinalis in the mussel Mytilus galloprovincialis cultured in Galicia (NW Spain). Dis Aquat Org, 21: 221-226.

Φώτης Γ, Κυριαζή-Παπαδοπούλου Α, Πνευματικάτος Η, Καλδρυμίδου Ε, Κανακοΰδης Γ, (1997) Πρόδρομος μελέτη του πρωτόζωου ως αιτία θανάτου των μυδιών Mytilus galloprovincialis στο Θερμαϊκό κόλπο θεσσαλονίκης. Δελτίον της Ελληνικής Κτηνιατρικής Εταιρείας 48(3): 121-125.

Grizel Η (1979) Marteilia refringens and oyster disease recent observations. Mar Fish Rev, 41 (1/2): 38-44.

Grizel Η (1985) Étude des récentes épizooties de l'huître plate (Ostrea edulis Linné) et leur impact sur l'ostréiculture bretonne. Thèse Doctorat es Sciences, Univ. Sciences et Techniques du Languedoc, Montpellier.

Grizel H, Comps M, Bonami JR, Cousserans F, Duthoit JL, Le Pennec MA (1974) Recherche sur l'agent de la maladie de la glande digestive de Ostrea edulis Linné. Sci Pêche, 240: 7-29.

Grizel H, Comps M, Raguenes D, Leborgne Y, Tige G, Martin AG (1982) Bilan des essais d'acclimatation d'Ostrea chilensis sur les côtes de Bretagne. Rev Trav Inst Pêches Marit, 46: 209-225.

Grizel H, Tige G (1979) Observations sur le cycle de Marteilia refringens. Haliotis, 8: 327-330.

Hine PM, Thorne Τ (2000) A survey of some parasites and diseases of several species of bivalve mollusc in northern Western Australia. Pis Aquat Org, 40: 67-78T

Kleeman SN, Adlard RD (2000) Molecular detection of Marteilia sydneyi, pathogen of Sydney rock oysters. Dis Aquat Org, 40:137-46.

Kleeman SN, Adlard RD, Lester RJG (2002a) Detection of the initial infective stages of the protozoan parasite Marteilia sydneyi in Saccostrea glomerata and their development through to sporogenesis. Int J Parasitol, 32: 767-784.

Kleeman SN, Le Roux F, Berthe F, Adlard RD (2002b) Specificity of PCR and in situ hybridization assays designed for detection of Marteilia sydneyi and M refringens. Parasitology, 125: 131-141.

Καραγιάννης Δ (2005) Παρασιτολογική μελέτη του καλλιεργούμενου δίθυρου μαλακίου Mytilus galloprovincialis L. Στο Θερμαϊκό κόλπο και επίδραση του πρωτόζωου Marteilia sp. στις φυσιολογικές του λειτουργίες. Μεταπτυχιακή Διπλωματική Εργασία, Αριστοτέλειο Πανεπιστήμιο θεσσαλονίκης, θεσσαλονίκη.

Lauckner G (1983) Diseases of Mollusca: Bivalvia. In: Ο. Kinne (ed.) Diseases of Marine Animals. Volume II: Introduction, Bivalvia to Scaphopoda. Biologische Anstalt Helgoland, Hamburg.

Le Roux F, Audermard C, Barnaud A, Berthe F, (1999) DNA probes as potential tools for the detection of Marteilia refringens. Mar Biotechnol, 1: 588-597.

Le Roux F, Lorenzo G, Peyret Ρ, Audemard C, Figueras A, Vivarès C, Gouy M, Berthe F (2001) Molecular evidence for the existence of two species of Marteilia in Europe. J Eukaryot

Microbiol, 48: 449-454.

Longshaw M, Feist SW, Matthews RA, Figueras A (2001) Ultrastructural characterisation of Marteilia species (Paramyxea) from Ostrea edulis, Mytilus edulis and Mytilus galloprovincialis in Europe. Dis Aquat Org, 44: 137-142.

Lopez-Flores I, De La Herran R, Garrido-Ramos MA, Navas JI, Ruiz-Rejon C, Ruiz-Rejon M (2004) The molecular diagnosis of Marteilia refringens and differentiation between Marteilia strains infecting oysters and mussels based on the rDNA IGS sequence. Parasitology, 129: 411-419.

Montes J, Longa MA, Lama A, Guerra A (1998) Marteiliosis of Japanese oyster (Crassostrea gigas) reared in Galicia NW Spain. Bull Eur Ass Fish Pathol, 18: 124-126.

Moyer MA, Blake Ν J, Arnold WS (1993) An ascetosporan disease causing mass mortality in the Atlantic calico scallop, Argopecten gibbus (Linnaeus, 1758). J Shellfish Res, 12: 305-310.

Norton JH, Perkins FP, Ledua E (1993) Marteilia-like infection in a giant clam, Trìdacna maxima, in Fiji. J Invertebr Pathol, 61: 328-330.

Novoa B, Posada D, Figueras A (2005) Polymorphisms in the sequences of Marteilia internal transcribed spacer region of the ribosomal RNA genes (ITS-1) in Spain: genetic types are not

related with bivalve hosts. J Fish Pis, 28(6): 331-338.

ΟΙΕ (2003a) Diagnostic Manual for Aquatic Animal Diseases. ΟΙΕ, Paris. Fourth edition.

ΟΙΕ (2003b) International Aquatic Animal Health Code. ΟΙΕ, Paris. Sixth edition.

Pascual M, Martin AG, Zampatti E, Coatanea D, Defossez J, Robert R (1991) Testing of the Argentina oyster, Ostrea puelchana in several French oyster farming sites. ICES Council Meeting Pap. CM 1991/K, 30.

Perkins FO (1976) Ultrastructure of sporulation in the European flat oyster pathogen, Marteilia refringens-taxonomic implications. J Protozool, 23: 64-74.

Perkins FO (1988) Parasite morphology, strategy and evolution. Structure of protistan parasites found in bivalve molluscs. Am Fish Soc Spec Pubi, 18: 93-111.

Perkins FO, Wolf PH (1976) Fine structure of Marteilia sydneyi sp. n.- Haplosporidian pathogen of Australian oysters. J Parasitol, 62: 528-538.

Pernas M, Novoa B, Tafalla C, Figueras (2000) Efficiency of different monoclonal antibodies in immunological assays developed for the detection of Marteilia sp. isolated from Mytilus galloprovincialis. Bull Eur Ass Fish Pathol, 20(5): 193-198.

Pernas M, Novoa B, Berthe F, Tafalla C, Figueras A (2001) Molecular methods for the diagnosis of Marteilia refringens. Bull Eur Ass Fish Pathol, 21(5): 200-209.

Peters R, Raftos D (2002) The effects of Marteilia sydneyi on the host defense responses of the Sydney rock oysters, Saccostrea glomerata. J Shellfish Res, 21(1): 437.

Poljansky GI (1992) Protozoology and the problem of species. J Protozool, 39(1): 177-180.

Παπουτσόγλου ΣΕ (1985) Κατασκευές ελεγχόμενης εκτροφής δίθυρων μαλακίων. Στο: Κατασκευές Υδατοκαλλιεργειών. Εκδόσεις Λιβάνη, Αθήνα: 254-284.

Rayyan Α (2003) Βιοτικές αλληλεπιδράσεις του καλλιεργούμενου εδώδιμου δίθυρου μαλακίου Mytilus galloprovincialis Lam. Στο Θερμαϊκό κόλπο. Διδακτορική διατριβή, Θεσσαλονίκη.

Renault Τ, Cochennec Ν, Chollet Β, (1995) Marteiliosis in American oysters Crassostrea virginica reared in France. Dis Aquat Org, 23:161-164.

Robledo JAF, Boulo V, Mialhe E, Despres B, Figueras A (1994) Monoclonal antibodies against sporangia and spores οι Marteilia sp. (Protozoa: Ascetospora). Dis Aquat Org, 18: 211-216.

Robledo JAF, Figueras AJ (1995) The effects of culture-site, depth, season and stock source on the prevalence of Marteilia refringens in cultured mussels (Mytilus galloprovincialis LMK.) from Galicia, Spain. J Parasitol, 81: 354-363.

Roubal FR, Masel J, Lester RJG (1989) Studies on Marteilia sydneyi, agent of QX disease in the Sydney rock oyster, Saccostrea commercialis, with implications for its life cycle. Aust J Mar Freshwater Res, 40: 155-167.

Sawidis G (2001) Health status in the Greek bivalve mollusk culture. Proceedings of annual meeting of EU National Reference Laboratories for Bivalve Mollusc Diseases, Brussels 14-15 November 2001.

Sawidis G (2002) Health status in the Greek bivalve mollusk culture. Proceedings of annual meeting of EU National Reference Laboratories for Bivalve Mollusc Diseases, La Tremblade (France) 23-26 September 2002.

Sprague V (1979) Classification of the Haplosporidia. Mar Fish Rev, 41: 40-44.

Tamburri NM, Wasson K, Masayasu M (2002) Ballast water deoxygenation can prevent aquatic introductions while reducing ship corrosion. Biological Conservation 103(3): 331-341

Teia dos Santos AM, Coimbra J (1995) Growth and production of raft-cultured Mytilus edulis L., in Ria de Aveiro: gonad symbiotic infestation. Aquaculture, 132: 195-211.

Van Banning Ρ J (1988) Management strategies to control diseases in the Dutch culture of edible oysters. In: Couch J.A., Fournie J.W. (Eds.). Advances in Fisheries Science, Pathobiology of marine and estuarine organisms. Boca Raton, CRC Press, 243-245.

Villalba A, Mourelle SG, Carballal MJ, Lopez MC (1993a) Effects of infection by the protistan parasite Martellici refringens on the reproduction of cultured mussels Mytilus galloprovincialis in Galicia (NW Spain). Dis Aquat Org, 17: 205-13.

Villalba A, Mourelle SG, Carballal MJ, Lopez MC, Azevedo C (1993b) Marteiliasis affecting cultured mussels Mytilus galloprovincialis of Galicia NW Spain: I. Etiology, phases of the infection, and temporal and spatial variability in prevalence. Dis Aquat Org, 16: 61-72.

Villalba A, Mourelle SG, Carballal MJ, Lopez MC (1997) Symbionts and diseases of farmed mussels Mytilus galloprovincialis throughout the culture process in the Rias of Galicia (NW Spain). Dis Aquat Org, 31: 127-139.

Virvilis C, Angelidis P, Photis G (2003) Presence of the parasite Marteilia sp. in the shellfish of the Thermaikos Gulf in northern Greece. Bull Eur Ass Fish Pathol, 23: 157-161.

Zrncic S, Le Roux F, Oraic D, Sostane Β, Berthe FCJ (2001) First record of Marteilia sp. in mussels Mytilus galloprovincialis in Croatia. Dis Aquat Org, 44: 143-148.


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