Genetic Polymorphisms of Cyp19 and Myostatin Genes in Turkish Indigenous Sheep Breeds

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Published: Jan 29, 2018
DOI: https://doi.org/10.12681/jhvms.15475
Keywords:
Cyp19 myostatin sheep SNP PCR-RFLP
I. AKIŞ
Istanbul University, Faculty of Veterinary Medicine, Department of Biochemistry
F. ESEN GÜRSEL
Istanbul University, Faculty of Veterinary Medicine, Department of Biochemistry
N. HACIHASANOĞLU ÇAKMAK
Istanbul Medipol University, Vocational School of Health Services, Medical Laboratory Techniques Program
G. ATMACA
Istanbul University, Faculty of Veterinary Medicine, Department of Biochemistry
H. YARDIBI
Istanbul University, Faculty of Veterinary Medicine, Department of Biochemistry
A. ATEŞ
Istanbul University, Faculty of Veterinary Medicine, Department of Biochemistry
M. H. DURAK
Dicle University, Faculty of Veterinary Medicine, Department of Biochemistry
K. ÖZTABAK
Dicle University, Faculty of Veterinary Medicine, Department of Biochemistry
Abstract

Growth and meat production traits are very important in sheep breeding. Cyp19 gene has a major role in reproductive activity and growth due to its function in estrogen synthesis. Another gene affecting growth traits is  Myostatin (MSTN) gene, which mainly regulates skeletal muscle growth. In this study allele frequencies of genetic polymorphism in Cyp19 and Myostatin genes were identified by PCR-RFLP method in five indigenous Turkish sheep breeds, Chiose, Imroz, Kivircik, Zom and Morkaraman. Digestion of Cyp19 gene with HaeIII only revealed uncut AA genotype and digestion of MSTN with DraI also revealed only uncut AA genotype. Both loci analyzed in this study were found to be monomorphic in five Turkish indigenous sheep breeds. These highly conserved parts of the two genes can be useful for molecular evolutionary studies in sheep. Further studies regarding association analysis of Cyp19 and MSTN in sheep should be conducted.

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