Mannheimia haemolytica and Bibersteinia trehalosi serotypes isolated from lambs with ovine respiratory complex in Spain

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Published: Dec 19, 2017
DOI: https://doi.org/10.12681/jhvms.15497
Keywords:
Birbersteinia trehalosi lamb Mannheimia haemolytica ORC ovine respiratory complex pneumonia serotyping
J. M. GONZALEZ
Gabinete Técnico Veterinario S.L., C/ Isla Conejera s/n.
D. LACASTA
Departamento de Patologia Animal, Facultad de Veterinaria, Universidad de Zaragoza
L. M. FERRER
Departamento de Patologia Animal, Facultad de Veterinaria, Universidad de Zaragoza
L. FIGUERAS
Gabinete Técnico Veterinario S.L., C/ Isla Conejera s/n.
G. ABADIE
AFSSA Sophia Antipolis, Laboratoire d’etude et de recherche des petits ruminants et des abeilles
M. DE LAS HERAS
Departamento de Patologia Animal, Facultad de Veterinaria, Universidad de Zaragoza
Abstract

Ovine respiratory complex causes significant economic losses in ovine production systems around the world. Mannhemia haemolytica and Bibersteinia trehalosi are the two most frequently isolated bacterial species. In this study, bacterial isolates of M. haemolytica and B. trehalosi isolated from dead lambs were serotyped by means of indirect haemagglutination, with the general aim to improve information in the preparation for candidate strains for relevant vaccines. In total, 84 isolates were serotyped. Eight different M. haemolytica serotypes and four different B. trehalosi serotypes were identified. The most frequent
M. haemolytica serotypes were A2 (29% of isolates), A12 (18%) and A1 (12%), while B. trehalosi were predominantly T4 (40% of isolates) and a group sharing characteristics of T3 and T15 serotypes (43%). Isolation of T4 serotype was associated with lack of consolidated lesions in lungs, whilst isolation of T3-T15 group was associated with lung consolidation. It is concluded that a potential vaccine to control the disease presented as a bacterin based on outer membrane proteins and leucotoxins of the organisms, it should contain the above, frequently implicated serotypes. Alternatively, vaccines based on iron-regulated proteins can be possible replacements.

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References
Ackermann MR, B rogden KA (2000) Response of the ruminant respiratory tract to Mannheimia (Pasteurella) haemolytica.
Microbes Infect 2:1079-1088.
Al-Darraji AM, Cutlip RC, Lehmkuhl HD, Graham DL, Kluge JP, Frank GH, (1982): Experimental infection of lambs with bovine respiratory syncytial virus and Pasteurella haemolytica: clinical and microbiologic studies. Am J Vet Res 43:236-240.
Angen Ø, Mutters R, Caugant DA, Olsen JE, B isgaard M (1999a) Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov. Int J Sys B acteriol 49:67-86.
Angen O, Quirie M, Donachie W, B isgaard M (1999b) Investigations on the species specificity of Mannheimia (Pasteurella)
haemolytica serotyping. Vet Microbiol 65:283-290.
Brogden KA, Lehmkuhl HD, Cutlip RC (1998) Pasteurella haemolytica complicated respiratory infections in sheep and goats. Vet Res 29:233-254.
Confer AW (1993) Immunogens of Pasteurella. Vet Microbiol 37:353- 368.
Cutlip RC, Lehmkuhl HD, B rogden KA (1993): Chronic effects of coinfection in lambs with parainfluenza-3 virus and Pasteurella haemolytica. Small Rumin Res 11:171-178.
Cutlip RC, Lehmkuhl HD, B rogden KA, Hsu NJ (1996) Lesions in lambs experimentally infected with ovine adenovirus serotype 6 and Pasteurella haemolytica. J Vet Diagn Invest 8:296-303.
Donachie W (2000) Pasteurellosis. In: Enfermedades de la oveja, 2nd edn. Editorial Acribia Zaragoza, pp. 230-238.
Fodor L, Varga J, Hajtos I, Molnar T (1999) Serotypes of Pasteurella haemolytica and Pasteurella trehalosi isolated from farm animals in Hungary. J Vet Med B Infect Dis Vet Public Health 46:241-247.
Gilmour NJ, Donachie W, Sutherland AD, Gilmour JS, Jones GE, Quirie M (1991) Vaccine containing iron-regulated proteins of Pasteurella haemolytica A2 enhances protection against experimental pasteurellosis in lambs. Vaccine 9:137-140.
González JM, De las Heras M, Ferrer JM, Figueras L, Garcia de Jalón JA, Lacasta D (2001) Las neumonías catarrales crónicas
influyen negativamente en los índices productivos del cordero tipo ternasco. Proceedings of the XXVI Jornadas Científicas de la Sociedad Espanola de Ovinotecnia y Caprinotecnia (Sevilla, Spain), pp. 742-748.
Hajtos I, Fodor L, Varga J, Malik G (1985) Pasteurella haemolytica serotypes and associated diseases in sheep flocks. Magyar Allatorvosok Lapja 40:473-479.
Lacasta D, De las Heras M, González JM, Ferrer JM, Arandia G (2004) Evolución de los procesos neumónicos en cuatro explotaciones de la provincia de Huesca a lo largo de tres años. Proceedings of the XXIX Jornadas Científicas de la Sociedad Espanola de Ovinotecnia y Caprinotecnia (Lleida, Spain), pp. 256-258.
Lacasta D, Ferrer LM, Ramos JJ, González JM, Heras Mdl (2008) Influence of climatic factors on the development of pneumonia in lambs. Small Rum Res 80:28-32.
Murray JE, Davies RC, Lainson FA, Wilson CF, Donachie W (1992) Antigenic analysis of iron-regulated proteins in Pasteurella haemolytica A and T biotypes by immunoblotting reveals biotypespecific epitopes. J Gen Microbiol 138:283-288.
Odendaal MW, Henton MM (1995) The distribution of Pasteurella haemolytica serotypes among cattle, sheep, and goats in South Africa and their association with diseases. Onderstepoort J Vet Res 62, 223-226.
Odugbo MO, Odama LE, Umoh JU, Makinde AA, (2003) Serotypes of Pasteurella haemolytica from pneumonic lungs of sheep in northern Nigeria. Small Rum Res 48:239-243.
Purdy CW, Cooley JD, Straus DC (1998): Cross-protection studies with three serotypes of Pasteurella haemolytica in the goat model. Curr Microbiol 36:207-211.
Sabri MY, Zamri-Saad M, Mutalib AR, Israf DA, Muniandy N (2000): Efficacy of an outer membrane protein of Pasteurella haemolytica A2, A7 or A9-enriched vaccine against intratracheal challenge exposure in sheep. Vet Microbiol 73:13-23.
Sanchis R, Polveroni G, Abadie G (1988) Serotyping of Pasteurella haemolytica: comparison and perfection of the antigens and techniques. Rev Med Vet 139:719-722.
Scott FM, Angus KW, Dewar P (1990) Pneumonia of lambs following inoculation of isolates of sheep herpesvirus (caprine herpesvirus 1) of different DNA genotypes. J Comp Pathol 102:111-117.
Villard L, Gauthier D, Lacheretz A, Abadie G, Game Y, Maurin F, Richard Y, B orges E, Kodjo A (2006) Serological and molecular comparison of Mannheimia haemolytica and Pasteurella trehalosi strains isolated from wild and domestic ruminants in the French Alps. Vet J 171:545-550.
Vougidou C.T., Sandalakis V., Psaroulaki A., Petridou E., Donachie W., Ekateriniadou L. (2012) Serotypic diversity and sequence variation of the ompA gene among Mannheimia haemolytica isolates from domestic ruminants. J Hell Vet Med Soc 63:13-24.