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Detection and Molecular Identification of Salmonella Strains Isolated from an Industrial Ostriches farm

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Poultry salmonellosis, one of the most prevalent diseases and major source of food-borne infections to humans due to consumption of poultry products is worldwide in distribution. The aim of this study was to investigate the incidence of salmonellosis in ostriches by culture and PCR , determination of antibiotic resistance pattern of the isolates and the infected ostriches antibody level. 87 fecal samples from one industrial ostrich farm with clinical signs of diarrhea, weight loss, mortality and reduced hatchability were collected and evaluated for presence of the Salmonella. Salmonella was isolated according to standard culture and biochemical tests. The Salmonella positive samples were serotyped with O and H antisera based on slide and tube agglutination tests. PCR was done for detection of serovars Infantis and Enteritidis. Then the antibiotic resistance against 14 antimicrobial agents were tested. The antibody level of the infected ostriches were measured by WIDAL agglutination test. Results indicated 9.1% (8 of 87) of ostriches were positive for Salmonella. Serotyping results showed 3 samples were serovar Infantis and 5 samples were serovar Enteritidis and PCR confirmed the serotyping results. All 8 samples were resistant to tetracyclin and ampicillin but sensitive to other antibiotics including ciprofloxacin, kanamycin, sultrim, cephalothin, norfloxacin, chloramphenicol, flumequine, nitrofurantoin, coamoxiclav, gentamicin, enrofloxacin and cefotaxime The results of WIDAL agglutination test indicated that all ostriches were negative except 8 Salmonella positive ostriches with the titres 1/80 to 1/360 for the O and 1/80 to 1/640 for the H antigens. To our knowledge this is the first study which reports the peresence of Salmonella Infantis in ostriches in Iran and more studies should be done to investigate this pathogen in ostriches herds of Iran.


Salmonella; ostriches; PCR; Iran

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Arshad MM, Rahbar MH, Boulton ML, Wells E, Wilkins MJ, Saeed A (2006) Risk factors for Salmonella Oranienburg outbreak in anursing home in Michigan. J Amer Geriat Socie 54: 715-717.

Bacci C, Paris A, Salsi A (2006) Genotypic and Phenotypic virulence features in Salmonella enterica strains isolated from meat. Annali Della Facolta Di Medicina Veterina Del Studi Di Parma 26: 165-174.

Barrow PA, Simpson JM, Lovell MA, Binns MM (1987) Contribution of Salmonella Gallinarum large plasmid toward virulence in fowl typhoid. Infect Immun 55: 388-392.

Bauer A, Kirby W, Sherris JC, Turck M (1996) Antibiotic susceptibility testing by a standardized single disk method. Amer J Clin Pathol 45(4): 493-496.

Chiari M, Zanoni M, Tagliabue S, Lavazza A, Alborali LG (2013) Salmonella serotypes in wild boars (Sus scrofa) hunted in northern Italy. Acta Vet Scandina 55: 421-424.

Chikami GK, Fierer J, Guiney DG (1985) Plasmid-mediated virulence in Salmonella Dublin demonstrated by use of a Tn5-oriT construct. Infect Immun 50: 420-424.

Clinical and Laboratory Standards Institute (CLSI) (2014) Performance Standards for Antimicrobial susceptibility Testing; Twenty-Fourth Informational Supplement. M100-S24. Wayne, PA: CLSI.

Guibourdenche M, Roggentin P, Mikoleit M, Fields PI, Bockemuhl J, Grimont, PA (2010) Supplement 2003–2007 (No. 47) to the White Kauffmann-Le Minor scheme. Res Microbiol 161(1):26-9.

Gulig PA, Curtiss RL (1987) Plasmid-mediated virulence in Salmonella Typhimurium. Infect Immun 55: 2891-2901.

Heffernan EJ, Fierer J, Chikami G, Guiney D (1987) Natural history- of oral Salmonella Dublin infection in BALB/c mice: effect of an 80-kilobase pair plasmid on virulence. J Infect Dis 155: 1254-1259.

Kardos G, Farkas T, Antal M, Nogrady N, Kiss I (2007) Novel PCR assay for identification of Salmonella enterica serovar Infantis. Lett Appl Microbiol 45: 421-425

Kaushik P, Anjay Kumari S, Kumar Bharti S, Dayal S (2014) Isolation and prevalence of Salmonella from chicken meat and cattle milk collected from local markets of Patna, India. Vet World 7(2): 62-65.

Libby SJ, Adams LG, Ficht TA, Allen C, Whitford HA, Buchmeier NA, Bossie S, Guiney DG (1997) The spv genes on the Salmonella Dublin virulence plasmid are required for severe enteritis and systemic infection in the natural host. Infect Immun 65: 1786-1792.

Madadgar O, Zahraei Salehi T, Tadjbakhsh H, Mahzounieh M, Feizabadi M (2008) Genomic and phenotypic evaluation of Salmonella Typhimurium and Salmonella Enteritidis in Iran. Comp Clin Pathol 17: 229-235.

Malorny B, Hoorfar J, Bunge C, Helmuth R (2003) Multicenter validation of the analytical accuracy of Salmonella PCR: towards an international standard. Appl Environment Microbiol 69: 290-296.

Mulvey MR, Boyd DA, Olson AB, Doublet B, Axel-Cloeckaert A (2006) The genetics of Salmonella genomic island 1. Microbes Infect 8: 1915-1922.

Murugkar HV, Rahman H, Kumar A, Bhattacharyya D (2005) Isolation, phagetyping and antibogram of Salmonella from man and animals in northeastern India. Ind J Medical Res 122: 237-242.

Neu HC (1992) The crisis in antibiotic resistance. Science 257: 1064- 1073.

Oludairo OO, Kwaga JKP, Dzikwi AA, Kabir J (2013) The genus Salmonella, isolation and occurrence in wildlife. Int J Microbiol Immunol Res 1: 47-52.

Pan TM, Liu YJ (2002) Identification of Salmonella Enteritidis isolates by polymerase chain reaction and multiplex polymerase chain reaction. J Microbiol Immunol Infect 35: 147- 151.

Pang T, Puthucheary S (1983) Significance and value of the Widal test in the diagnosis of typhoid fever in an endemic area. J Clin Pathol 36: 471-475.

Quinn PJ, Carter ME, Markey PK, Carter GR (1994) Clinical Veterinary Microbiology. London: Wolfe publishing.

Rahn K, De Grandis SA, Clarke RC, Mc Ewen SA, Galane JE, Ginocchio C, Curtiss R, Gyles CL (1992) Amplification of an invA gene sequence of Salmonella Typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. Molecular Cellular Probes 6: 271- 279.

Ramya P, Madhavarao T, Venkateswara Rao L (2012) Study on the incidence of Salmonella Enteritidis in poultry and meat samples by cultural and PCR methods. Vet World 5(9): 541-545.

Shetty AK, Shetty IN, Furtado ZV, Antony B, Boloor R (2012) Antibiogram of Salmonella Isolates from Blood with an Emphasis on Nalidixic Acid and Chloramphenicol Susceptibility in a Tertiary Care Hospital in Coastal Karnataka: A Prospective Study. J Lab Physicians 4: 74-77.

Silva-Hidalgo G, Ortiz-Navarrete VF, Alpuche-Aranda CM, Rendón- Maldonado JG, López-Valenzuela M, Juárez-Barranco F, López-Moreno HS (2012) Non-typhi Salmonella serovars found in Mexican zoo animals. Res Vet Sci 93: 1132-1135.

Soumet C, Ermel G, Rose N, Rose V, Drouin P, Salvat G, Colin P (1999) Evaluation of a multiplex PCR assay for simultaneous identification of Salmonella spp. Salmonella Enteritidis and Salmonella Typhimurium from environmental swabs of poultry houses. Lett Appl Microbiol 28: 113- 117.

Willke A, Ergonul O, Bayar B (2002) Widal test in diagnosis of typhoid fever in Turkey. Clin Diag Lab Immunol 9: 938–941.

Witte W (1998) Medical consequences of antibiotic use in agriculture. Science 1998; 279: 996-997.

Woodward MJ, Kirwan SES (1996) Detection of Salmonella Enmteritidis in eggs by the polymerase chain reaction. Vet Rec 138: 411-413.


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