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Serological survey of paratuberculosis in dairy cattle in Garfagnana district (Tuscany)

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A. GALIERO, F. FRATINI, P. BRAVI, B. TURCHI, E. CASANOVI, D. CERRI
A. GALIERO, F. FRATINI, P. BRAVI, B. TURCHI, E. CASANOVI, D. CERRI

Abstract


Mycobacterium avium subsp. paratuberculosis (Map) is the agent of a chronic, progressive granulomatous enteritis in ruminants known as Johne’s disease or paratuberculosis. Nowadays, the interest regarding this pathogen is increasing not only because Map causes economic losses, but it has also been suggested as a potential risk factor for the development of human diseases such as Crohn disease, autoimmune diseases like type-1 diabetes, sarcoidosis, multiple sclerosis and Hashimoto’s thyroiditis. The aim of our study was to determine the presence of paratuberculosis in Garfagnana (Tuscany, Italy) where raw milk is not submitted to pasteurization, but it is directly sold to consumers or it is destined for cheese making without the employment of heat treatment. The survey was conducted in Garfagnana district where there are 17 herds which produce and market bovine milk for direct human consumption. Serum samples (n=162) were obtained form 16 herds and were analyzed performing ELISA ID screen® Paratuberculosis Indirect screening test (ID.VET, Montpellier, France) and positive samples were tested with ELISA ID screen® Paratuberculosis Indirect confirmation test (ID.VET, Montpellier, France), according to the instructions provided by the manufacturer. The analysis performed by ELISA revealed that the true seroprevalence was 29.1% at the herd level and 4.6% at the animal level. In our opinion, although ELISA is a very useful tool to screen herds for paratuberculosis, more studies should be carried out in our territory combining serological data with cultural and PCR analysis.

Keywords


paratuberculosis; cattle; ELISA; Tuscany; Italy

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References


Carta T, Álvarez J, Pérez de la Lastra JM, Gortázar C (2013) Wildlife and paratuberculosis: A review. Res Vet Sci 94(2):191-7.

Cenci-Goga BT, Vescera F, Paolotto P, McCrindle CME, Roberti U (2010) Seroprevalence of Mycobacterium avium subspecies paratuberculosis in cows in Umbria, Italy. Vet Rec 167(15):577-8.

Cerri D, Cantile C, Ebani VV, Montagnese M, Voltini B, Arispici M (2002) Diagnosis of paratuberculosis in naturally infected goats. New Microbiol 25(2):131-137.

Fry MP, Kruze J, Collins MT (2008) Evaluation of four commercial enzyme-linked immunosorbent assays for the diagnosis of bovine paratuberculosis in chilean dairy herds. J Vet Diagn Invest 20(3):329-32.

Galiero A, Fratini F, Turchi B, Colombani G, Nuvoloni R, Cerri D (2015) Detection of Mycobacterium avium subsp. Paratuberculosis in a sheep flock in Tuscany. Trop Anim Health Prod 47(8):1567-1571.

Galiero A, Fratini F, Mataragka A, Turchi B, Nuvoloni R, Ikonomopoulos J, Cerri D (2016) Detection of Mycobacterium avium subsp. paratuberculosis in cheeses from small ruminants in Tuscany. Int J Food Microbiol 217:195-199.

Giacometti F, Serraino A, Finazzi G, Daminelli P, Losio MN, Arrigoni N, Piva S, Florio D, Riu R, Zanoni RG (2012) Sale of raw milk in Northern Italy: Food safety implications and comparison of different analytical methodologies for detection of foodborne pathogens. Foodborne Pathog Dis 9(4):293-297.

Hasonova L, Pavlik I (2006) Economic impact of paratuberculosis in dairy cattle herds: A review. Vet Med 51(5):193-211.

Italian Ministry of Health (2013) Accordo tra il Governo, le Regioni e le Province autonome di Trento e di Bolzano sulle Linee guida per l’adozione dei piani di controllo e certificazione nei confronti della Paratubercolosi bovina. Rep. Atti n. 146/CSR. 17 ottobre 2013. Italian Ministry of Health, Rome, Italy.

Koriem KMM (2016) Multiple sclerosis: New insights and trends. Asian Pac J Trop Biomed 6(5):429-40.

Lillini E, Bitonti G, Gamberale F, Cersini A (2005, August). Prevalence of bovine paratuberculosis in the Latium region (Italy). In Proceedings 8th International Colloquium on Paratuberculosis August 14-18.

Nielsen SS, Toft N (2008) Ante mortem diagnosis of paratuberculosis: A review of accuracies of ELISA, interferon-γ assay and faecal culture techniques. Vet Microbiol 129(3-4):217-235.

Nielsen SS, Toft N, Okura H (2013). Dynamics of Specific Anti-Mycobacterium avium Subsp. paratuberculosis Antibody Response through Age. PLoS ONE, 8(4):e63009.

OIE, 2014. Paratuberculosis (Johne’s disease), OIE Terrestrial Manual 2014, Chapter 2.1.11.

Pozzato N, Capello K, Comin A, Toft N, Nielsen SS, Vicenzoni G, Arrigoni N (2011) Prevalence of paratuberculosis infection in dairy cattle in Northern Italy. Prev Vet Med 102(1):83-86.

Ricchi M, Savi R, Bolzoni L, Pongolini S, Grant IR, De Cicco C, Cerutti G, Cammi G, Garbarino CA, Arrigoni N (2016) Estimation of Mycobacterium avium subsp. paratuberculosis load in raw bulk tank milk in Emilia-Romagna Region (Italy) by qPCR.

MicrobiologyOpen 5(4):551-559.

Savi R, Ricchi M, Cammi G, Garbarino C, Leo S, Pongolini S, Arrigoni N (2015) Survey on the presence of Mycobacterium avium subsp. Paratuberculosis in ground beef from an industrial meat plant. Vet Microbiol 177(3-4):403-408.

Sechi P, Paolotto P, McCrindle CME, Cenci-Goga BT (2013) Seroepidemiological study of Johne’s-disease in dairy cattle in Umbria, Italy. Ital J Anim Sci 12(2):196-199.

Sechi LA, Dow CT (2015) Mycobacterium avium ss. Paratuberculosis Zoonosis - The Hundred Year War - Beyond Crohn’s Disease. Front Immunol doi: 10.3389/fimmu.2015.00096.

Sergeant ESG (2017) Epitools epidemiological calculators. Ausvet Pty Ltd. http://epitools.ausvet.com.au.

Serraino A, Bonilauri P, Arrigoni N, Ostanello F, Ricchi M, Marchetti G, Bonfante E, Albonetti S, Giacometti F (2014a) Quantitative risk assessment of Mycobacterium avium subsp. Paratuberculosis survival in pasteurized milk in three dairy plants in Italy. Food Control 45:138-142.

Serraino A, Arrigoni N, Ostanello F, Ricchi M, Marchetti G, Bonilauri P, Bonfante, E, Giacometti F (2014b) A screening sampling plan to detect Mycobacterium avium subspecies paratuberculosis-positive dairy herds. J Dairy Sci 97(6):3344-3351.

Stevenson K (2015) Genetic diversity of Mycobacterium avium subspecies paratuberculosis and the influence of strain type on infection and pathogenesis: A review. Vet Res 46(1):1-13.

Whan L, Ball HJ, Grant IR, Rowe MT (2005) Occurrence of Mycobacterium avium subsp. paratuberculosis in untreated water in Northern Ireland. Appl Environ Microbiol 71(11):7107-7112.

Wells SJ, Wagner BA (2000) Herd-level risk factors for infection with Mycobacterium paratuberculosis in US dairies and association between familiarity of the herd manager with the disease or prior diagnosis of the disease in that herd and use of preventive measures. J Am Vet Med Assoc 216(9):1450-1457.

Whitlock RH, Wells SJ, Sweeney RW, Van Tiem J (2000) ELISA and fecal culture for paratuberculosis (Johne’s disease): Sensitivity and specificity of each method. Vet Microbiol 77(3-4):387-98.


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