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Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran

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H MOOMIVAND, SA POURBAKHSH, M JAMSHIDIAN
H MOOMIVAND, SA POURBAKHSH, M JAMSHIDIAN

Abstract


In ostriches, mycoplasmas are generally associated with respiratory diseases and causes rhino-tracheitis, airsacculitis and inflammation of the upper respiratory tract. The aim of current study was the isolation and identification of pathogenic mycoplasmas in ostrich farms of Iran by the use of PCR and culture methods. In this study, mycoplasmas were isolated from ostrich slaughterhouse; 114 samples were collected from ostriches with respiratory signs and were cultured and PCR methods along with alignment were used to detect the mycoplasmas. For this purpose lung, trachea and air sacs were evaluated. The results indicated that 21.05% of samples were positive in PCR assayand from them 7.89% and 14% was M. gallisepticum and M. synoviae, respectively. The highest rate of M. gallisepticum and M. synoviae was detected in lung, airsacs and trachea. Alignment analysis demonstrated that the M. gallisepticum strains detected in our study have 97% homology to 06/14, 05/14 and 16S strains. In addition, M. synoviae strains have 99% and 98% homology to MSR-812, MSR-795 and MSR-1019 strains. One of the M. synoviae strains has 82% homology to ABSfsdMS2011 strain. The results of our study showed that ostriches in Iran were infected with chicken mycoplasmas but the pathogenesis of them in ostrich respiratory should be further evaluated.

Keywords


mycoplasma; ostriches; isolation; pcr; sequencing

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References


Al‐Ankari, A R S, Bradbury, J M (1996) Mycoplasma Iowae: A Review. Avian Pathol 2: 205-229.

Bagheri, H, Doosti, A, Arshi, A (2011) Detection of Mycoplasma Gallisepticum in Chaharmahal Va Bakhtiari Province Poultry Using Pcr. Global Vet 1: 54-59.

Blackall, P J, Christensen, H, Beckenham, T, Blackall, L L, Bisgaard, M (2005) Reclassification of Pasteurella Gallinarum,[Haemophilus] Paragallinarum, Pasteurella Avium and Pasteurella Volantium as Avibacterium Gallinarum Gen. Nov., Comb. Nov., Avibacterium Paragallinarum Comb. Nov., Avibacterium Avium Comb. Nov. And Avibacterium Volantium Comb. Nov. Int J Syst Evol Microbiol 1: 353-362.

Botes, A, Peyrot, B, Olivier, A, Burger, W, Bellstedt, D (2005) Identification of Three Novel Mycoplasma Species from Ostriches in South Africa. Vet Microbiol 3: 159-169.

Bradbury, J M, Morrow, C J (2008) Avian Mycoplasmas. In: PoultryDisease. ed. Saunders Elsevier Philadelphia, USA: pp 220-234.

Cadman, H F, Kelly, P J, Zhou, R, Davelaar, F, Mason, P R (1994) A Serosurvey Using Enzyme-Linked Immunosorbent Assay for Antibodies against Poultry Pathogens in Ostriches (Struthio Camelus) from Zimbabwe. Avian Dis 3: 621-625.

Calnek, B W, Barnes, H J (1997). Diseases of Poultry. 10th Ed., Iowa State University Press, Ames, pp. Cline, J. L.,, Turner, K S, O’connor, R J, Gomez, J, L. B.,, Kleven, S H (1997) Determination of Colonisation of Mycoplasma Gallisepticum and Mycoplasma Synoviae in Ostriches. Am. Ostrich, Special Annu. Res.: 12-13.

Elhamnia, F, Banani, M, Shokri, G, Pourbakhsh, S, Ashtari, A (2016) Detection of Mycoplasma Synoviae Infection in Broiler Breeder Farms of Tehran Province Using Pcr and Culture Methods. Archives of Razi Institute 2: 75-81.

Ghaleh Golab Behbahan, N, Asasi, A, Afsharifar, A, Pourbakhsh, S (2005) Isolation and Detection of Mycoplasma Gallisepticum by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism. Iranian Journal of Veterinary Research of Univiversity Shiraz 1: 35-41.

Huchzermeyer, F W (1994). Ostrich Diseases. Onderstepoort Veterinary Institute, pp: 23.

Kleven, S H (2008) Control of Avian Mycoplasma Infections in Commercial Poultry. Avian Dis 3: 367-374.

Lauerman, L H, Hoerr, F J, Sharpton, A R, Shah, S M, Van Santen, V L (1993) Development and Application of a Polymerase Chain Reaction Assay for Mycoplasma Synoviae. Avian Dis 3: 829-834.

Nascimento, E R, Pereira, V, Nascimento, M, Barreto, M (2005) Avian Mycoplasmosis Update. Revista Brasileira de Ciência Avícola 1: 1-9.

Panangala, V S, Gresham, M M, Morsy, M A (1992) Antigenic Heterogeneity in Mycoplasma Iowae Demonstrated with Monoclonal Antibodies. Avian Dis 1: 108-113.

Peccati, C, Grilli, G, Gallazzi, D, Rampin, T (1996). Serological Survey in Ostriches in Northern Italy. Improving our Understanding of Ratites in a Farming Environment, Dalton-Ellis Hall, University of Manchester, England.

Peighambari, S, BozorgmehrifardÙ, M, Hosseini, H, Pourbakhsh, S, Razzazian, M (2006) Random Amplified Polymorphic DNA (Rapd) Fingerprinting of Mycoplasma Gallisepticum Isolates from Chickens. Archives of Razi Institute 2: 67.

Shane, S M (1998) Infectious Diseases and Parasites of Ratites. Veterinary Clinics of North America: Food Animal Practice 3: 455-483.

Shivaprasad, H (1993) Neonatal Mortality in Ostriches: An Overview of Possible Causes. Annual Conference of the Association of Avian Veterinarians, Proceedings: 282-293.

Smith, C (1993) Ostrich Chick Survival Presents Challenge. Journal of the American Veterinary Medical Association (USA).

Swayne, D E (1998). Laboratory Manual for the Isolation and Identification of Avian Pathogens. American Association of Avian Pathologists, University of Pennsylvania, pp.

Tebyanian, H, Mirhosseiny, S H, Kheirkhah, B, Hassanshahian, M (2014) Isolation and Identification of Mycoplasma Synoviae from Suspected Ostriches by Polymerase Chain Reaction, in Kerman Province, Iran. Jundishapur journal of microbiology 9.

Van Kuppeveld, F, Van Der Logt, J, Angulo, A, Van Zoest, M, Quint, W, Niesters, H, et al. (1992) Genus-and Species-Specific Identification of Mycoplasmas by 16s Rrna Amplification. Appl Environ Microbiol 8: 2606-2615.

Verwoerd, D (2000) Ostrich Diseases. Revue scientifique et technique (International Office of Epizootics) 2: 638-661.


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