Characterization of a novel recombination event in the Deformed wing bee virus polymerase gene

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DOI: https://doi.org/10.12681/jhvms.16071
Keywords:
Deformed wing virus RdRp honeybees Varroa recombination
G GORAS
Laboratory of Apiculture-Sericulture, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, Aristotle University, Greece
IGA RUETHER
Laboratory of Apiculture-Sericulture, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, Aristotle University, Greece
CH TANANAKI
Laboratory of Apiculture-Sericulture, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, Aristotle University, Greece
S GOUNARI
Institute of Mediterranean Forest Ecosystems and Forest Products Technology, NAGREF, 11528 Athens, Greece
V LIOLIOS
Laboratory of Apiculture-Sericulture, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, Aristotle University, Greece
D KANELIS
Laboratory of Apiculture-Sericulture, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, Aristotle University, Greece
N ARGENA
Laboratory of Apiculture-Sericulture, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, Aristotle University, Greece
M RODOPOULOU
Laboratory of Apiculture-Sericulture, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, Aristotle University, Greece
EM KARAZAFIRIS
Laboratory of Apiculture-Sericulture, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, Aristotle University, Greece
A THRASYVOULOU
Laboratory of Apiculture-Sericulture, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, Aristotle University, Greece
Abstract

Honeybee populations are known to be infected by numerous viruses. Reverse transcription-PCR (RT-PCR) of regions of the RNA-dependent RNA polymerase is often used to diagnose the presence in apiaries and also to classify the type of virus detected. In this report, through analysis of the RdRp gene, we describe a novel recombination event in the DWV genome. Similarity plot analysis amplified from hundred positive individuals identified a previously undescribed recombination point in the 5’ region of the polymerase gene. To our knowledge this is the first description of recombination in the DWV polymerase gene and highlights the continuous genetic evolution of these viruses.

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