Significance of selected biochemical markers in predicting the outcome of schistosomiasis

Published: May 2, 2018
S. mansoni Mice Oxidative stress Histopathology

This study aimed to correlate the histopathological changes in mice liver with alterations either in liver tissue antioxidants enzymes (catalase and reduced glutathione (GSH)), oxidative stress marker (malondialdehyde (MDA)) or in serum liver function parameters (Alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein (T.P.) and albumin) to predict the outcome of schistosomiasis. Fourty male Swiss albino mice were used in this study and infected with Schistosoma mansoni for 2, 4, 6 and 8 weeks (8 mice for each group), while, the uninfected mice were used as negative control. Liver tissue antioxidants enzymes, oxidative stress marker and serum liver function parameters were determined in coincide with the liver tissue histopathological changes. All selected biochemical makers showed a strong significant positive correlation (p < 0.05) with liver histopathology score except serum albumin and liver tissue catalase enzyme. The last two parameters exhibited negative correlation with liver histopathology score. These results revealed that the more increase in the level of AST, ALT, T.P. and globulin in serum or liver tissue MDA and GSH indicating severs histopathological changed into the affected liver and hopeless prognosis is expected. On contrary, the increase in albumin level in serum or catalase level in liver tissue of affected patient/animal demonstrating mild liver histopathological changes. Subsequently, good prognosis and response to anti-schistosomal treatment will be predictable. This study opens the way to predict the outcome of schistosomiasis through easy and rapid biochemical test. Therefore, other studies are required to apply such correlation with other biochemical parameters especially that synthesized into the liver.

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Abdallahi OMS, Hanna S, de Reggi M, Gharib B (1999) Visualization of oxygen radical production in mouse liver in response to infection with Schistosoma mansoni. Liver 19:495–500.
Aebi H, (1984) Catalase in vitro. Methods enzymol 105:121-126.
Beutler E, Duron, O, Kelly, MB (1963) Improved method for the determination of blood glutathione. J Lab Clin Med 61:882-8.
Cheeseman K (1993) Mechanism and effect of lipid peroxidation. Molec.Asp. Med: 14 ,191 -197.
Cheever AW, Lenzi JA, Lenzi HL, Andrade ZA (2002) Experimental models of Schistosoma mansoni infection. Mem Inst Oswaldo Cruz 97: 917–940.
Page CR 3rd, Etges FJ, Ogle JD (1972) Experimental prepatent schistosomiasis mansoni: quantitative analyses of proteins, enzyme activity and free amino acids in mouse serum. Exp parasitol 31: 341-349.
Coutinho EM, Silva FL, Barros AF, Araújo RE, Oliveira SA, Luna CF, Barbosa AA Jr, Andrade ZA (2007) Repeated infections with Schistosoma mansoni and liver fibrosis in undernourished mice. Acta Tropica 101: 15–24.
Curtis J and Minchella DJ (2000) Schistosome population genetic structure: when clumping worms is not just splitting hairs. Parasitol Today 16:68-71.
Despommier D, Gwadz R, Hotez P, Knirsch C (2000) Parasitic diseases, 4th ed. New York: Apple Trees Productions. 345 p.
Dessein AJ, Hillaire D, Elwali NE, Marquet S, Mohamed-Ali Q, Mirghani A, Nenri S, Abd Elhameed AA, Saeed OK, Magzoub MM, Abel L (1999) Severe hepatic fibrosis in Schistosoma mansoni infection is controlled by a major locus that is closely linked to the interferon-gamma receptor gene. Am J Hum Genet 65: 709–721.
El-Sokkary GH, Reiter RJ, Tan DX, Kim SJ, Cabrera J (1999) Inhibitory effect of melatonin on products of lipid peroxidation resulting from chronic ethanol administration. Alcohol Alcohol 34: 842-850.
Fenwick A, Savioli L, Engels D, Bergquist RN, Todd MH, (2003) Drugs for the control of parasitic diseases: current status and development in schistosomiasis. Trends Parasitol 19:509–15.
Gharib B, Abd-Allah OM, Dessein H, De-Reggi M (1999) Development of eosinophil peroxidase activity and concomitant alteration of the antioxidant defenses in the liver of mice infected with Schistosoma mansoni. J Hepatol 30:594-602.
Gul M, Kutay FZ, Temocin S, Hanninen O (2000) Cellular and clinical implications of glutathione. Indian J Exp Biol 38:625-634.
Hamburger J, Turetski T, Kapeller I, Deresiewicz R (1991) Highly repeated short DNA sequences in the genome of Schistosoma mansoni recognized by s species-specific probe. Mol Biochem Parasitol 44 :73–80.
Harfoush MA, Soliman HA, (2003) Clinicopathological studies on fascioliasis in naturally infected cattle. Kafer El- sheikh Vet Med J 1:799-809.
Hirota M1, Inoue M, Ando Y, Hirayama K, Morino Y, Sakamoto K, Mori K, Akagi M (1989) Inhibition of stress induced gastric injury in the rat by glutation. Gastroenterology 97: 853-859.
Jatsa HB, Kenfack CM, Simo DN, Feussom NG, Nkondo ET, Tchuente LT, Tsague CD, Dongo E, Kamtchouing P (2015) Schistosomicidal, hepatoprotective and antioxidant activities of the methanolic fraction from Clerodendrum umbellatum Poir leaves aqueous extract in Schistosoma mansoni infection in mice BMC Complement Altern Med 15: 248.
Katz N, Chaves A, Pellegrino J (1972) A simple device for quantitative stool thick-smear technique in Schistosomiasis mansoni, Rev. Inst. Med. Trop. Sao Paulo 14 :397–400.
Lew H, Pyke S, Quintanilha A (1985) Changes in the glutation status of plasma, liver, skeletal muscle following exhaustive exercise in rats FEBS. Letters 185: 262-266.
Liang YS, John BI, Boyd DA (1987) Laboratory cultivation of Schistosoma vector snails and maintenance of schistosome life cycles. Proceeding of the 1st Sino-American Symposium 1:34-48.
Mbuh JV, Julie M (2005) Serological changes in Goat experimentally infected with fasciola gigantic in buea sub division of S.W.P. Cameron Vet Parasitol 131:255-259.
Murray R (1984) Alanine aminotranseferase Clin Chem The C.V., Mossby CO,st Louis Toronto Princeton 1268-1273 and 425 .
Nare B, Smith JM, Prichard RK (1990) Schistosoma mansoni: levels of antioxidants and resistance to oxidants increase during development. Exp Parasitol. 70:389-97.
Paradis V, Kollinger M, Fabre A, Holstge T, Poynard P, Bedossa (1997) In situ detection of lipid eroxidation by products in chronic liver diseases. Hepatology 26:135-143.
Pascal M, Abd-Allah O M, Elwali NE, Mergani A, Quarshi MA, Magzoub M, De-Reggi M, Gharib B (2000) Hyaluronate levels and markers of oxidative stress in the serum of Sudanese subjects at risk of infection with Schistosoma mansoni. Trans R Soc Trop Med Hyg 94:66-70.
Sen C (1997) Nutritional biochemistry of cellular glutathione. J Nutr Biochem 8: 660-672.
Shaheen H, Shalab YK, Farid Z, Campbell J, Kamal K (1996) Parasite Specific iso type and sub class anti body profiles during acute prepatent Human schistosomiasis. Exp Parasitol 82:222–4.
Song Z, Cawthon D, Beers K, Bottje W (2000) Hepatic and extra hepatic stimulation of glutathione release in to plasma by norepinephrenic in vivo. Poultry Science 79: 1632 -1639.
Steinmann P, Keiser J, Bos R, Tanner M, Utzinger J (2006) Schistosomiasis and water resources development: systematic review, meta-analysis, and estimates of people at risk. Lancet Infect Dis 6:411–25.
Teixeiral R, Ferreira MD, Coelho PM, Filho GB, Lambertuccil JR (1996) Pyogenic liver abscesses and acute schistosomiasis mansoni: report on 3 cases and experimental study transaction of the royal society Trop Med And Hyg 90: 280.
Wang Y, Holmes E, Nicholson JK, Cloarec O, Chollet J, Tanner M, Singer BH, Utzinger J (2004) Metabonomic investigations in mice infected with Schistosoma mansoni: an approach for biomarker identification. Proceedings of the Natural Academy of Sciences of United States of America 101: 12676–12681.
Yoshioka T, Kawada K, Shimada T, Mori M (1979) Lipid peroxidation in maternal and cord blood and protective mechanism against activated oxygen toxicity in blood. Am J Obstet Gynecol 135; 372-376.