Polymorphism of ompH gene of Pasteurella multocida serotype A strains isolated in Iran

PDF
DOI: https://doi.org/10.12681/jhvms.16439
Keywords:
Pasteurella multocida RFLP-PCR ompH gene
M. OULAD
Payame Noor University, Faculty of Tehran-Center Rey
Y. TAHAMTAN
Shiraz Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO)
N. SOHRABI
Department of Biology, Payame Noor University
Abstract

One of the most frequent causes of respiratory infection and death in sheep and goats is Pasteurella multocida. In humans, it has been associated with diseases of the respiratory tracts, arthritis, osteomyelitis and meningitis. Outer membrane protein H (OmpH) has a role in immunogenicity and pathogenicity of P. multocida. The aim of this study was to characterize the genetic diversity of ompH gene of a panel of P. multocida serotype A strains isolated in sheep. Forty P. multocida serotype A strains isolated in previous study were selected and analyzed by restriction fragment length polymorphism (RFLP) of a species-specific PCR assay. RFLP amplified fragment produced five different cleavage patterns. On the basis of combinations resulting from ompH gene digestion, the 40 P. multocida isolates were classified in six RFLP type. It seems that isolates with variants genetic profile represent different pathogenecity. New vaccine formulation should consider multivariants of P. multocida in order to confer a wider protection.

Article Details
  • Section
  • Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
Download data is not yet available.
References
Bhimani M. P, Ashish R, Bhanderi B, Mathakiya R. A (2014) Isolation, identification and molecular characterization of Pasteurella multocida isolates obtained from emu (Dromaius novaehollandiae) in Gujarat state, India. Vet Arh, 84: 411-419.
Chang LB, Heller S, Marco T, Siedlik L (2012) A molecular approach to the characterization and intra-species differentiation of porcine Pasteurella multocida strains associated with pneumonia and progressive atrophic rhinitis. Ame Ass Swine Vet, 5: 159-165.
Daneshlari S, TahamtanY, Kargar M (2013) Molecular analysis of virulence factors of Pasteurella multocida from sheep and goats. OJVR, 17:675-684.
Davies LR, Mac Corquodale R, Baillie S, Caffrey B (2003) Characterization and comparison of Pasteurella multocida strains associated with porcine pneumonia and atrophic rhinitis. J Med Microbiol, 52:59–67.
Dousse F, Thomann A, Brodard I, Bozena M. Korczak, Schlatter Y, Kuhnert P, Miserez R, Frey J (2008) Routine phenotypic identification of bacterial species of the family Pasteurellaceae isolated from animals. J Vet Diagn Invest, 20:716–724.
Ghanizadeh A, Jabbari A. R, Shayegh J, Banihashemi A (2015) Genotyping of Pasteurella multocida ovine and bovine isolates from Iran based on PCR-RFLP of ompH gene. Arc Razi Ins, 70:151-156.
Harper M, Boyce JD, Adler B (2006) Pasteurella multocida pathogenesis: 125 years after Pasteur. FEMS Microbiol Lett, 265:1–10.
Hussaini J, Mhm N, Masyitah N, Mahmood A, Ismail A. S (2013) Alternative animal model for Pasteurella multocida and aemorrhagic septicaemia. Biomed Res, 24: 263-266.
Jabbari A.R, Esmaelzadeh M, Moazeni Ju Gh (2006) Polymerase chain reaction typing of Pasteurella multocida capsules isolated in Iran. Iranian J Vet Res, 16:50-55.
La R G, De Carolis E, Sali M, Papacchini M, Riccardi C, Mansi A(2006) Genetic diversity of bacterial strains isolated from soils, contaminated with polycyclic aromatic hydrocarbons, by 16S rRNA gene sequencing and amplified fragment length polymorphism fingerprinting. Microbiol Res, 161: 150-157.
Lee G, Kim YB, Kwon V( 2007) Outer membrane protein H for protective immunity against Pasteurella multocida. J Microbiol, 45:179-84.
Luo Y, John RG, Mark WJ, Robert EW, Mangeet B, I-hsing NC(1997) Cloning and characterization of the major outer membrane protein gene (ompH) of Pasteurella multocida X-73. Ame Soc Microbiol, 179:7856–7864.
Michael J and Abbott S. L (2007) 16S rRNA Gene Sequencing for Bacterial Identification in the Diagnostic Laboratory: Pluses, Perils, and Pitfalls. J Clin Microbiol, 45: 2761–2764.
Miguel A, Rojas F, vaca S, Reyes-Lo, pez M, Garza M, aguilar-Romero F(2014) Outer membrane vesicles of Pasteurella multocida contain virulence factors. Microbiol Open, 3: 711–717.
Prabhakar TG, Prabhakar P, Venkadabadi T (2010) Molecular characterization of Pasteurella multocida isolated from an incidence of sheep pasteurellosis in karamadai hill tract of Tamil Nadu. Tamilnadu J Vet Anim Sci, 6: 81-87.
Prasannavadhana S, Kumar S, Thomas P, Narayan Sarangi L, Gupta S, Priyadarshini A, Konasagara Nagaleekar V, Pal Singh V( 2014) Outer Membrane Proteome Analysis of Indian Strain of Pasteurella multocida Serotype B: 2 by Maldi-TOF/MS Analysis. Sci World J, 8: 1-10.
Sahragard I, Tahamtan Y, Valadan M, Hyati M, Shirazi Z (2011) Development of rapid PCR method for simultaneous identification of species, specific capsular type, and toxigenicity of Pasteurella sp. isolates. Comp Clin Pathol, 2: 1-4.
Sellyei B, Ivanics E, Magyar T (2012) Characterization of avian Pasteurella multocida strains with PCR-RFLP analysis of the oomph gene. Microbiol Open, 61: 48-64.
Shayegh J, Atashpaz S, Hejazi MS (2008) Virulence genes profile and typing of ovine Pasteurella multocida. Asian J Anim Vet Adv, 3: 206-213.
Singh R, Tewari K, Packiriswamy N, Marla S, Velagapudi D, Rao P(2011) Molecular characterization and computational analysis of the major out membrane protein (ompH) n of Pasteurella multocida P52. Vet Arh 81:211-222.
Tabatabaei M, Liu Z, Finucane A, Parton R, Coote J (2002) Protective immunity conferred by attenuated aroA derivative of Pasteurella multocida B:2 strains in a mouse model of hemorrhagic septicemia. Infect Immun 70: 3355-3362.
Tahamtan Y, Amrabadi O, Shahryari R (2016) Identification of pasteurella multocida and molecular diagnosis of haemorrhagic septicaemia in Iranian camels. Revue Méd167, 5-6, 126-132.
Tahamtan* Y, Hayati M, Namavari MM (2014) Isolation and Identification of Pasteurella multocida by PCR from sheep and goats in Fars province, Iran . Arch Razi Ins, 69: 89-93.
Tahamtan Y, Mirghafari H (2016) Classification capsular PCR typing and genetic diversity of Pasteurella multocida isolated from sheep and goats in Iran using Bam HI, Hind III restriction endonuclease enzymes by PCR-RFLP. Trop Biomed, 33:535-542.
Tahamtan Y, Sahragard I (2016) Characterization, ribotyping and capsular PCR typing of Pasteurella multocida isolated from Iranian sheep and goats. Int J Molecul Clin Microbiol, 6: 602-6.
Tan HY, Nagoor NH, Sekaran SD (2010) Cloning, expression and protective capacity of 37 kDa outer membrane protein gene (ompH) of Pasteurella multocida serotype B: 2. Trop Biomed, 27:430–441.
Tshikhudo PH, Nnzeru R, Ntushelo K, Mudau F(2013) Bacterial species identification getting easier. Afr J Biotechnol, 12: 5975-5982.
Williams PP, Hall MR, Rimler RB (1990) Host response to Pasteurella multocida turbinate atrophy toxin in swine. J Vet Res, 54:157–163.
Zamrisaad M, Effendy AWM, Maswati MA, Salim N, Sheikhomar AR (1996) The goet as a model for studies of pneumonic pasteurellosis caused by Pasteurella multocida. Bri Vet J, 152: 453-458.
Most read articles by the same author(s)