Prevalence and antimicrobial resistance of Salmonella isolated from bovine and ovine samples in slaughterhouses of Algiers, Algeria

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DOI: https://doi.org/10.12681/jhvms.16441
Keywords:
Salmonella slaughterhouse prevalence serovars antimicrobial resistance
S. NOUICHI
Food Hygiene and Quality Assurance System Laboratory, High National Veterinary School of Algiers
R. OUATOUAT
Food Hygiene and Quality Assurance System Laboratory, High National Veterinary School of Algiers
H. Y. CAN
Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Mustafa Kemal University
L. MEZALI
High National Veterinary School of Algiers
C. BELKADER
Pasteur Institute of Algeria
M. OUAR- KORICHI
Pasteur Institute of Algeria
S. BERTRAND
National Reference Centre for Salmonella and Shigella, Bacterial Diseases Division, Communicable and Infectious Diseases, Scientific Institute of Public Health (WIV-ISP)
Z. CANTEKIN
Department of Microbiology, Faculty of Veterinary Medicine, Mustafa Kemal University
T. Y. HAMDI
Food Hygiene and Quality Assurance System Laboratory, High National Veterinary School of Algiers
Abstract

The aims of our work are to estimate the prevalence of Salmonella isolated from carcasses and feces of cattle and sheep in the two biggest slaughterhouses in Algiers, Algeria, and to characterize the obtained strains by serotyping and antimicrobial resistance testing. The detection of Salmonella was performed by the conventional culture method and isolates were confirmed by PCR. Susceptibility to antibiotics was carried out by agar disc diffusion method. The results showed that 10.17% of samples were Salmonella positive. Carcass samples were more contaminated than fecal samples. Serotyping of the 84 Salmonella isolates has enabled to identify 10 different serovars; the most predominant was S. Muenster. The invA gene was detected in 96.43% of isolates whereas all S. Typhimurium strains were positive for spy gene. Sixty-eight (80.95%) isolates were resistant to at least one of the 28 antibiotics tested and exhibited 17 different antimicrobial resistance patterns. The most frequently observed resistance was to streptomycin (69.05%). While 22.62 % of the isolates were MDR, two S. Typhimurium showed an‘‘ACSSuT’’ pentaresistance pattern. Considering the importance of this group of bacteria for public health, Salmonella control is necessary at several steps of food production to ensure safe products for consumers.

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References
Abbassi-Ghozzi I, Jaouani A, Hammami S, Martinez-Urtaza J, Boudabous A, Gtari M (2012) Molecular analysis and antimicrobial resistance of Salmonella isolates recovered from raw meat marketed in the area of ‘‘Grand Tunis’’, Tunisia. Pathol Biol 60:49–54.
Addis Z, Kebede N, Sisay Z, Alemayehu H, Yirsaw A, Kassa T (2011) Prevalence and antimicrobial resistance of Salmonella isolated from lactating cows and in contact humans in dairy farms of Addis Ababa: a cross sectional study. BMC Infect Dis 11:222.
Ahmed AM, Shimamoto T (2014) Isolation and molecular characterization of Salmonella enterica, Escherichia coli O157:H7 and Shigella spp. From meat and dairy products in Egypt. Int J Food Microbiol 168-169:57-62.
Ateba CN, Mochaiwa B (2014) Use of invA gene specific PCR analysis for the detection of virulent Salmonella species in beef products in the North West Province, South Africa. J Food Nutr Res 2(6):294-300.
Aouf A, Messai Y, Salama MS, Aboushady HM, El-Anany MG, Alouache S, Bakour R (2011) Resistance to β-lactams of human and veterinary Salmonella isolates in Egypt and Algeria. Afr J Microbiol Res 5(7):802-808.
Bahnass MM, Fathy AM, Alamin MA (2015) Identification of human and animal Salmonella spp. isolates in Najran region and control of it. Int J of Adv Res 3(1):1014-1022.
Bordonaro R, McDonough PL, Chang Y-F, Mohammed HO (2015) The potential risk associated with foodborne pathogens in watersheds: Salmonella spp. in dairy cattle. J Water Resource Prot 7:476-484.
Bounar-Kechih S, Hamdi TM, Mezali L, Assaous F, Rahal K (2012) Antimicrobial resistance of 100 Salmonella strains isolated from Gallus gallus in 4 wilayas of Algeria. Poultry Sci 91(5):1179-1185.
Bouzidi N, Aoun L, Zeghdoudi M, Bensouilah M, Elgroud R, Oucief I, Granier SA, Brisabois A, Desquilbet L, Millemann Y (2012). Salmonella contamination of laying-hen flocks in two regions of Algeria. Food Res Int 45(2):897–904.
Can HY, Elmali M, Karagöz A, Öner S (2014) Detection of Salmonella spp., Salmonella Enteritidis, Salmonella Typhi and Salmonella Typhimurium in cream cakes by polymerase chain reaction (PCR). Med Weter 70(11):689-692.
CDC Centers for Disease Control and Prevention (2014) Foodborne diseases active surveillance network (FoodNet): FoodNet surveillance report for 2012 (Final Report). Atlanta, Georgia: U.S. Department of Health and Human Services, CDC. Retrieved from https://www.cdc.gov/foodnet/PDFs/2012_annual_report_508c.pdf
CLSI (Clinical Laboratory Standard Institute, 2008). Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved standard –Third Edition M31-A3 .Vol.28 N° 8. Replaces M31-A2. Vol.22 N°06. February 2008.
Dabassa A, Bacha K (2012) The prevalence and antibiogram of Salmonella and Shigella isolated from abattoir, Jimma town, South West Ethiopia. Inter J Pharm Biomed Res 3(4):143-148.
Dong P, Zhu L, Mao Y, Liang R, Niu L, Zhang Y, Li K, Luo X (2014) Prevalence and profile of Salmonella from samples along the production line in Chinese beef processing plants. Food Control 38:54-60.
EFSA European Food Safety Authority (2014) The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2012. EFSA Journal 12(2):3547.
EFSA European Food Safety Authority (2015) EU summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2013. EFSA Journal 13(2):4036.
El Allaoui A, Filali FR, Ameur N, Nassri I, Oumokhtar B, Aboulkacem A, Essahale A, Derouich A, Bouchrif B (2014) Prevalence, antibio-resistance and risk factors for Salmonella in broiler turkey farms in the province of Khémisset (Morocco). J World Poult Res 4(1):20-29.
Elgroud R, Zerdoumi F, Benazzouz M, Bouzitouna-Bentchouala C, Granier SA., Fremy S, Brisabois A, Dufour B, Millemann Y (2009) Characteristics of Salmonella contamination of broilers and slaughterhouses in the region of Constantine (Algeria). Zoonoses and Public Health 56:84-93.
Elgroud R, Granier SA, Marault M, Kerouanton A, Lezzar A, Bouzitouna- Bentchouala C, Brisabois A, Millemann Y (2015) Contribution of avian Salmonella enterica isolates to human salmonellosis cases in Constantine (Algeria). BioMed Res Int 2015:352029.
Ginocchio CC, Rahn K, Clarke RC, Galan JE (1997) Naturally occurring dele- tions in the centisome 63 pathogenicity island of environmental isolates of Salmonella spp. Infect Immun 65:1267-1272.
Hendriksen RS, Vieira AR, Karlsmose S, Danilo MA, Wong LF, Jensen AB, Wegener HC, Aarestrup FM (2011) Global monitoring of Salmonella serovar distribution from the World Health Organization Global Foodborne Infections Network Country Data Bank: results of quality assured laboratories from 2001 to 2007. Foodborne Pathog Dis 8(8):1-14.
Kagambèga A, Lienemann T, Aulu L, Traoré AS, Barro N, Siitonen A, Haukka K (2013) Prevalence and characterization of Salmonella enterica from the feces of cattle, poultry, swine and hedgehogs in Burkina Faso and their comparison to human Salmonella isolates. BMC Microbiol 13: 253.
Karmi M (2013) Detection of virulence gene (invA) in Salmonella isolated from meat and poultry products. Int J Genetics 3(2):07-12.
Le Hello S, Bekhit A, Granier SA., Barua H, Beutlich J, Zając M, Münch S, Sintchenko V, Bouchrif B, Fashae K, Pinsard JL, Sontag L, Fabre L, Garnier M, Guibert V, Howard P, Hendriksen RS., Christensen JP, Biswas PK, Cloeckaert A, Rabsch W, Wasyl D, Doublet B, Weill FX (2013). The global establishment of a highly-fluoroquinolone resistant Salmonella enterica serotype Kentucky ST198 strain. Front Microbiol 4:395.
Malorny B, Hoorfar J, Bunge C, Helmuth R (2003) Multicenter validation of the analytical accuracy of Salmonella PCR: towards an international standard. Appl Environ Microbiol 69:290-296.
Manoj J, Singh MK., Singh YP (2015) The role of poultry in food borne salmonellosis and its public health importance. Adv Anim Vet Sci 3(9):485-490.
Maysa AIA, Abd-Elall AMM (2015) Diversity and virulence associated genes of Salmonella enterica serovars isolated from wastewater agricultural drains, leafy green producing farms, cattle and human along their courses. Revue Med Vet 166(3-4):96-106.
Mezali L, Hamdi TM (2012) Prevalence and antimicrobial resistance of Salmonella isolated from meat and meat products in Algiers (Algeria). Foodborne Pathog Dis 9(6):522-529.
Nickerson CA, CURTISS R (1997) Role of sigma factor RpoS in initial stages of Salmonella Typhimurium infection. Infect Immun 65(5):1814-1823.
Nouichi S, Hamdi TM (2009) Superficial bacterial contamination of ovine and bovine carcasses at El-Harrach slaughterhouse (Algeria). Eur J Sci Res 38:474-485.
Olsen JE, Aabo S, Rasmussen OF, Rossen L (1995) Oligonucleotide probes specific for the genus Salmonella and for Salm. typhimurium. Lett Appl Microbiol 20:160-163.
Rahn K, De Grandis SA, Clarke RC, McEwen SA, Galan JE, Ginocchio C, Curtiss III R, Gyles CL (1992) Amplification of an invA gene sequence of Salmonella Typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. Mol.Cell.Probes 6:271-279.
Sallam KI, Mohammed MA, Hassan MA, Tamura T (2014) Prevalence, molecular identification and antimicrobial resistance profile of Salmonella serovars isolated from retail beef products in Mansoura, Egypt. Food Control 38:209-214.
Singh P, Mustapha A (2014) Development of a real-time PCR melt curve assay for simultaneous detection of virulent and antibiotic resistant Salmonella. Food Microbiol 44:6-14.
Smith SI, Fowora MA, Atiba A, Anejo-Okopi J, Fingesi T, Adamu ME, Omonigbehin EA, Ugo-Ijeh MI, Bamidele M, Odeigah P (2015). Molecular detection of some virulence genes in Salmonella spp isolated from food samples in Lagos, Nigeria. Am J Anim Vet Sci 3(1):22-27.
Tafida SY, Kabir J, Kwaga JKP, Bello M, Umoh VJ, Yakubu SE., Nok AJ, Hendriksen R (2013) Occurrence of Salmonella in retail beef and related meat products in Zaria, Nigeria. Food Control 32:119-124.
Teklu A, Negussie H (2011) Assessment of risk factors and prevalence of Salmonella in slaughtered small ruminants and environment in an export abattoir, Modjo, Ethiopia. American -Eurasian J Agric & Environ Sci 10(6): 992-999.
Van Cauteren D, Jourdan-da Silva N, Weill FX, King L, Brisabois A, Delmas G, Vaillant V, de Valk H (2009) Outbreak of Salmonella enterica serotype Muenster infections associated with goat’s cheese, France, March 2008. Euro Surveill 14(31), pii: 19290.
Van Kessel JS, Karns JS, Perdue ML (2003) Using a Portable Real-Time PCR Assay to Detect Salmonella in Raw Milk. J Food Prot 66(10):1762–1767.
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