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Seroprevalence of Neospora caninum in dairy cows in Belgrade city area, Serbia

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The protozoan parasite Neospora caninum is one of the most important abortifacient pathogen in cattle. Serological investigations are often used in order to estimate seroprevalence in herds. Aims of our study were to determine the seroprevalence among aborting and non-aborting dairy cattle in Belgrade city area as well as epidemiological factors that are important for N. caninum infection. Using commercial ELISA kit, we examined 188 sera. Out of 188, 142 samples originated from pregnant (non-aborting) cows from five farms, while 46 were from cows who had aborted. Overall seroprevalence was 25% (48/188). Seroprevalence was significantly higher (p≤0.05) in aborting than in non-aborting group of cows (37% and 21.1% respectively). At least one positive sample was detected on four (80%) out of five examined farms while seroprevalence among farms varied from 0 to 43.5%. On all examined farms crucial epizootiological factors (presence of dogs and low biosecurity measures) that favor the maintenance and spreading of the infection were identified. Our study revealed the presence of N. caninum antibodies in population of dairy cows in Belgrade city area. Infection is established in enzootic pattern on examined farms and high seroprevalence among aborting cows suggests that N. caninum could be important abortifacient pathogen.


Neospora caninum; seroprevalence; dairy cow; Serbia

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Almeira S, Lopez-Gatius F (2015) Markers related to the diagnosis and to the risk of abortion in bovine neosporosis. Res Vet Sci 100:169-175.

Conraths FJ, Gottstein B (2007) Neosporosis. In: Protozoal abortion in farm ruminants: Guidelines for diagnosis and control, Athenaeum Press, Gateshead: pp 42-122.

Corbellini LG, Driemeier D, Cruz CFE, Gondim LFP, Wald V (2002) Neosporosis as a cause of abortion in dairy cattle in Rio Grande do Sul, Southern Brazil. Vet Parasitol 103:195-202.

Cvetojević Đ, Kureljušić B, Jezdimirović N, Radanović O, Jakić-Dimić D, Veljović Lj, Ivetić V (2013) Usage of PCR in diagnosis of Neospora caninum induced abortions in cattle. In: Proceedings of the Scientific symposium “Reproduction of domestic animals”, Divčibare, Serbia: pp 179-180.

Cvetojević Đ, Savić B, Stanojević S, Kureljušić B, Jezdimirović N, Bojković-Kovačević S, Pavlović M, Katić M, Jakić-Dimić D (2014) Cattle abortions caused by contagious infectious agents on PKB corporation’s farms during 2014. In: Proceedings of XXIX Conference of Agronomists, Veterinarians, Technologists and Agricultural Economists, Belgrade, Serbia: pp 1-6.

Dubey JP (2003) Review of Neospora caninum and neosporosis in animals. Korean J Parasitol 41:1-16.

Dubey JP, Schares G (2006) Diagnosis of bovine neosporosis. Vet Parasitol 140:1-34.

Dubey JP, Schares G, Ortega-Mora LM (2007) Epidemiology and Control of Neosporosis and Neospora caninum. Clin Microbiol Rev 20:323-367.

Dubey JP, Schares G (2011) Neosporosis in animals—The last five years. Vet Parasitol 180:90-108.

Gavrilović P, Živulj A, Todorović I, Jovanović M, Parunović J (2013) Investigation of importance of Neospora caninum in aetiology of abortion in dairy cows in Serbia. Revue Méd Vét 164:100-104.

Hornok S, Edelhofer R, Hajtos I (2006) Seroprevalence of neosporosis in beef and dairy cattle breeds in northeast Hungary. Acta Vet Hung 54:485-491.

Innes EA, Wright S, Bartley P, Maley S, Macaldowie C, Esteban-Redondo I, Buxton D (2005) The host-parasite relationship in bovine neosporosis. Vet Immunol Immunopathol 108:29-36.

Kacar C, Gokce HI, Akca A, Gunsor O, Kaya S (2012) Seroprevalence of Neospora caninum in cows with abortion history and in dogs sharing the same area in the Kars Region, Turkey. Revue Méd Vét 163:343-347.

Klun I, Nikolić A, Vujanić M, Ivović V, Bobić B, Djurković-Djaković O (2008) Pilot study of Neospora caninum seroprevalence in cattle in Serbia. In: Program & Abstract Book of 10th European Multicolloquium of Parasitology, Paris, France: pp 192.

Klun I (2014) Seroepizootiology and molecular diagnosis of Neospora caninum infection in cattle in Serbia, PhD Thesis, Faculty of veterinary medicine, University of Belgrade, Serbia.

Kurtede A, Ural K (2009) Serological status, epidemiology and prevalence of bovine Neospora caninum infection in Turkey: a review of published studies. Revue Méd Vét 160:244-251.

Kuruca Lj, Spasojević-Kosić Lj, Simin S, Savović M, Lauš S, Lalošević V (2013) Neospora caninum antibodies in dairy cows and domestic dogs from Vojvodina, Serbia. Parasite 20:40.

Mitrea IL, Enacheschu V, Radulescu R, Ionita M (2012) Seroprevalence of Neospora caninum infection on dairy cattle in farms from southern Romania. J Parasitol 98:69-72.

Ould-Amrouche A, Klein F, Osdoit C, Mohammed HO, Touratier A, Sanaa M, Mialot J (1999) Estimation of Neospora caninum seroprevalence in dairy cattle from Normandy, France. BMC Vet Res 30:531-538.

Pavičić Lj, Lalošević V, Spasojević-Kosić Lj, Lauš S, Simin S (2011) Seroprevalence of Neospora caninum in dogs. Contemporary agriculture 60:453-457.

Sager H, Fischer I, Furrer K, Strasser M, Waldvogel A, Boerlin P, Audige L, Gottstein B (2001) A Swiss case-control study to assess Neospora caninum-associated bovine abortions by PCR, histopathology and serology. Vet Parasitol 102:1-15.

Savović M, Lalošević V, Simin S, Pavičić Lj, Boboš S (2012) Seroprevalence of Neospora caninum in dairy cows with reproductive disorders in Vojvodina province, Serbia. Lucrari Ştiintifice Medicina Veterinara 65:161-166.

Vidić B, Grgić Ž, Savić S, Bugarski D (2011) Neospora caninum causes abortions in cows. In: Proceedings of the Scientific Symposium “Reproduction of Domestic Animals”, Divčibare, Serbia: pp 133-134.

Wang C, Wang Y, Zou X, Zhait Y, Gao J, Hou M, Zhui X (2010) Seroprevalence of Neospora caninum Infection in Dairy Cattle in Northeastern China. J Parasitol 96:451-452.

Wisniewski M, Cabaj W, Moskwa B, Wedrychowicz H (2002) The first detection of Neospora caninum DNA in brains of calves in Poland. Acta Vet-Beograd 52:393-400.

Wouda W, Bartels CJM, Moen AR (1999) Characteristic of Neospora caninum- associated abortion storms in dairy herds in the Netherlands. Theriogenology 52:233-245.


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