Comparative effects of addition of superoxide dismutase and reduced glutathione on cryopreservation of Sahiwal bull semen

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DOI: https://doi.org/10.12681/jhvms.19619
Keywords:
Semen Superoxide Dismutase Reduced Glutathione Sahiwal Bull
A. Murtaza
Department of Theriogenology, Faculty of Veterinary Science, University of Agriculture Faisalabad, Pakistan
M. Ahmad
Department of Theriogenology, Faculty of Veterinary Science, University of Agriculture Faisalabad, Pakistan
M. Zubair
Department of Veterinary Clinical Sciences, Faculty of Veterinary and Animal Sciences, The University of Poonch Rawalakot, Pakistan
S. Umar
Department of Theriogenology, Faculty of Veterinary Science, University of Agriculture Faisalabad, Pakistan
A. Mushtaq
Institute of Microbiology, Faculty of Veterinary Science, University of Agriculture Faisalabad, Pakistan
A.H.S.T. Gul
Department of Pathology, Faculty of Veterinary Science, University of Agriculture Faisalabad, Pakistan
A. U. Khan
Department of Clinical Studies, Faculty of Veterinary and Animal Sciences, PMAS Arid Agriculture University, Rawalpindi, Pakistan
Abstract
The present study aimed to investigate effects of superoxide dismutase (SOD) and reduced glutathione (GSH) on the quality of frozen-thawed semen of Sahiwal bulls. Semen was collected twice a week for 8 weeks by artificial vagina from six Sahiwal bulls, kept at the Semen Production Unit Qadirabad, Sahiwal-Pakistan. After gross and microscopic evaluation, qualifying semen ejaculates were divided into 10 equal aliquots and diluted in extenders enriched with no antioxidants (control); or supplemented with either SOD (50, 100 and 200 IU/mL), or GSH (0.5, 1 and 2 mM) or their combinations (50 IU/mL SOD and 0.5 mM GSH, 100 IU/mL SOD and 1 mM GSH and 200 IU/mL SOD and 2 mM GSH). Samples were then frozen and stored in liquid nitrogen at -196°C for 24 h. The following parameters were evaluated for semen quality: post-thawed sperm motility, viability, acrosome and membrane integrity. According to the results, sperm motility, viability, acrosome and membrane integrity were significantly (P<0.05) higher in samples treated either with 100 IU/mL of SOD; 1 mM and 2 mM of GSH or 50 IU/mL of SOD plus 0.5 mM of GSH. In conclusion, semen quality might be improved by supplementing semen extenders with 100 IU/mL of SOD; 0.5 and 1 mM of GSH and combination of 50 IU/mL and 0.5 mM of SOD and GSH, respectively.
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