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Advanced diagnosis of vibriosis among some marine fishes in lake Temsah, Egypt

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IAM EISSA, S. N. ABOU-ELGHEIT, A. A. DESSUKI, A.A.I. HASSANIN, L. T. MOSTAFA
IAM EISSA, S. N. ABOU-ELGHEIT, A. A. DESSUKI, A.A.I. HASSANIN, L. T. MOSTAFA

Abstract


Vibriosis is a serious disease affecting marine water fishes. The present study was applied on 320 marine fishes of four equal different species (Sparus auratus, Mugil cephalus, Mugil seheli and Tilapia zillii) each 80. Fishes were collected seasonally and randomly from Lake Temsah in Ismailia governorate from September

2015 to August 2016. The signs and lesions of severe septicemia, hemorrhages and ulcerations were observed among the examined fishes. Isolated bacteria were observed as yellow and green pigmented colonies on TCBS media, and as creamy colored colonies on TSA media with 2.5% NaCl concentration. The causative agent was identified as vibrio sp. Concerning gene expression, most isolates were molecularly identified using the pvsA gene primers giving a product size of 338-bp size and 348-bp for V. alginolyticus and V. parahemolyticus respectively. The highest prevalence of vibriosis was recorded in T. zillii (63.75%), M.seheli (37.5%) then M.cephalus and Sparus auratus (28.75%) while the total prevalence was (39.69%). The highest seasonal prevalence was recorded in summer (81.25%) followed by spring (35%) then autumn (23.75%) and winter (18.75%). The highest prevalence of organ specifity was in liver, kidneys then spleen and gills. The histopathological studies showed activation of melanomacrophage centers, degeneration, necrosis and congestion in liver, kidney, spleen, and gills.


Keywords


Marine fishes; Vibriosis; pvsA gene; Histopathological exam

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References


Actis, L. A.; Tolmasky, M. E. and Crosa, J. H. (1999): Vibriosis. In: Woo, P.T.K., Bruno, D.W. (Eds.), Fish Diseases and Disorders, vol. 3. CAB Intern., Publ., United Kingdom, pp. 523– 558.

Adebayo-Tayo, B. C.; Okonko, I. O.; John, M. O.; Odu, N. N.; Nwanze, J. C. and Ezediokpu, M. N. (2011): Occurrence of Potentially Pathogenic Vibrio Species in Sea Foods Obtained from Oron Creek. Advances in Biological Research 5 (6): 356-365.

Alcaide E., (2003): Numerical taxonomy of Vibrionaceae isolated from cultured amberjack (Serioladumerili) and surrounding water, Curr. Microbiol.46:184–189.

Alicia, E.; Toranzo, T.; Magarinos, B. & Romalde, J. L. (2005): A review of the main bacterial fish diseases in mariculture systems. Aquac., 246: 37– 61.

Anzaldi, L.L., and Skaar, E.P. (2010): Overcoming the heme paradox: heme toxicity and tolerance in bacterial pathogens. Infect Immun78:4977–4989.

Austin B. and Austin D. A. (2007): Bacterial fish pathogens Diseases of farmed and wild fish B. Fourth Edition. ISBN 978-1-4020-6068-7; Springer Dordrecht Berlin Heidelberg New York.

Balebona, M.C; Zorrilla, I.; Morinigo,A . and Borrego, J.J. (1998): survey o bacterial pathologies affecting farmed gilt-head seabream (sparusaurataL.) in southwestern Spain from 1990 to 1996. Aquaculture, 166;19-35.

Buller, N.B. (2004): bacteria from fish and other Aquatic Animals: Apractical Identification Manual. CABI Publishing, Cambridge.

Crosa, J. H. (1997): Signal transduction and transcriptional and post transcriptional control of iron-regulated genes in bacteria. Microbiol. Mol. Biol. Rev.61:319–336.

Cruickshank, R.; Duguid, J. P.; Marmion, B. P. & Swain, R.H. (1982): Medical Microbiology, 12th Ed., Vol. 11: The Practice of medical microbiology. Churchill living stone, Edinburg, London.

Devi R, Surendran P, Chakraborty K (2009): Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from shrimp farms along the southwest coast of India. World J Microbiol Biotechnol 25:2005–2012.

Eissa IA.M., Derwa H.I., Maather El-Lamei, Amina Desuki, Mona S. Zaki, Hasna El-Sheshtawy (2013): Iron in water and some marine fishes in relation to vibriosis at Lake Temsah Life Science Journal 2013;10(3) http://www.lifesciencesite.comeissavet29@yahoo.com

El-Bassiony (2001): Study on vibriosis in some cultured fish in Egypt. PhD., Fish Dis. &Hyg., Fac. Vet. Med., Alex. Uni.

Elgendy,M.Y., M. Abdelsalam , M. Moustafa , A.M. Kenawy and A.Seida, (2015) Caligus elongates and photobacterium damselae subsp pisicida concomitant infections affecting broodstock European seabass, Dicentrarchus labrax , with special reference to histopathological responses. J. Aquacult. Res. Dev., Vol.6.10.4172/2155-

1000346.

-Enany M. El.; Ibrahim H. M.; Abou El Atta, M. El.; El Adawy, M. M. (2011): Bacteriological and histopathological studies on some bacterial pathogens causing diseases In cultured Mugil capito fish in Ismailia Governorate.

Farmer Ш, J.J. and Hickman Brenner, F.W. (1992): The genera Vibrio and Photobacterium In: Balows, A, TruEper, H.G.,Dworkin, M.,Harder, W.,& Schleifer, K.H.(Eds.). (1992), The Prokaryotes Vol.П (pp.2952-3011). New York, NY: Springer Verlag, 2nd ed.

Golomazou, E.; Athanassopoulou, F.; Vagianou, S.; Sabatakou, O. Tsantilas, H.; Rigos, G. &kokkokiris, L. (2006): Diseases of White Sea Bream (DiplodussargusL.) Reared in Experimental and Commercial Conditions in Greece Turk. J. Vet. Anim. Scin., 30: 389-

Kolkovski,S, Kolkovski J . Int. Aquafeed (2011): 14(2), 28-31.

Kustusch, J. R.; Kuehl, J. C. and Crosa. H. G (2011): Power plays: iron transport and energy transduction in pathogenic vibrios. Biometals. 24(3): 559–566.

Larsen, M.H., Boesen, H.T., (2001): Role of flagellum and chemotactic motility of Vibrio anguillarum for phagocytosis by and intracellular serviva in fish macrophages. FEMS Microbiol. Lett. 203, 149–152.

Lee, K.K. (1995): Pathogenesis studies on Vibrio alginolyticus in the grouper, Epinephelus malabaricus Bloch et Schneider. Microbial Pathogenesis.19, 39-48.

Lee, J. K.; Jung, D.W.; Yoon, K.S.; Yoon, S.K. &Kwak, N. S. (2006): Effect of diluent salt concentration and pH on the Enumeration of Vibrio parahaemolyticus by direct plating on selective agar. Food Sci. Biotechnol. 15: 866–870.

Lipp E.K., Huq. A., Colwell. R.R., (2002): Effects of global climate on infectious disease: The cholera model, Clin. Microbiol. Rev.15:757-770.

Ming-Xia Chen, He-Yang Li, Gang Li, and Tian-Ling Zheng ((2011: Distribution of Vibrio alginolyticus like species in Shenzhen coastal waters, China, Braz J Microbiol. 42(3): 884–896.

Roberts RJ. “Fish pathology” 3rd Edition, (2001): Bailliere tindall, London England.

Roberts, R.J. (2001a): Fish pathology, 3rd ed. W.B. Saunders, Philadelphia, PA.

Robert, B., Moeller, Jr. (2012):”Bacterial fish Diseases” California Animal Health and Food Safety Laboratory System University of California.

Sadek, S. (2000): Sea bream culture in Egypt; status, constraints and potential. Fish Physiol. Bioch., 22: 171-178.

Sakata, T., Hattori, M., (1988): Characteristics of Vibrio vulnificus isolated from diseased tilapia (Saratherodon niloticus). Fish Pathol.23, 33– 40.

Stephens, F. J., Raidal, S. R., Buller, N. and Jones, B. (2006): Infection with Photobacterium damselae subspecies damselae and Vibrio harveyi in snapper, Pagrus auratus with bloat. Austrilian Veterinary J, 84(5): 173-177.

Thompson JR, Randa MA, Marcelino LA, Tomita-Mitchell A, Lim E, Polz MF (2004): Diversity and dynamics of a north Atlantic coastal Vibrio community. Appl. Environ. Microbiol., 70: 4103-4110.

Tolmasky ME, Actis LA, Crosa JH. (1988): Genetic analysis of the iron uptake region of the Vibrio anguillarum plasmid pJM1: molecular cloning of genetic determinants encoding a novel trans activator of siderophore biosynthesis. J Bacteriol.1988;170:1913–1919.

Tomotaka Tanabe, Tatsuya Funahashi, Hiroshi Nakao, Shin-Ichi Miyoshi, Sumio Shinoda, and Shigeo Yamamoto (2003): Identification and Characterization of Genes Required for Biosynthesis and Transport of the Siderophore Vibrioferrin in Vibrio parahaemolyticus. Faculty of Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan.

Untergasser, A., H. Nijveen, X. Rao, T. Bisseling and R. Geurts (2007): Primer3Plus, an enhanced webinterface to Primer3. Nucl. Acids Res., 35: W71-W74. DOI: 10.1093/nar/gkm306.

Wafeek M, Abou El Atta, M. and Ebrahem, G. (2007): Heavy metals and bacterial distribution in different organs of Grey mullet (Mugil cephalus) cultured in different environmental conditions. Egypt J. Aquat. Biolo &Fish, Vol. 11, No3; 1125-1144.


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