Effects of diet supplementation with different level of Celmanax® in rainbow trout (Oncorhyncus mykiss)

Celmanax® Rainbow trout (Oncorhynchus mykiss) Haematological Biochemical parameters Mannanoligosaccharides
The aim of this study was to evaluate the effect of a prebiotic (Celmanax®) containing yeast cell wall with mannan oligosaccharides on the haematological and serum biochemical parameters in rainbow trout. Three levels of prebiotic (0, 0.1, 0.5 and 1 %) were mixed into pellets. Fish (19.08±1.45 g) were fed a supplemented commercial diet for 60 days. Blood samples were colected from the onset and on days 30 and 60 of the trial to measure the haematological and serum biochemical parameters in rainbow trout. The results showed significant differences in haemoglobin, mean corpuscular volume,mean corpuscular haemoglobin concentration, white blood cells and neutrophil count between control and all test groups (p<0.05). The highest and the lowest white blood cells and neutrophil count (on day 60) were observed in the 0.1 %, prebiotic-received and control groups, respectively. Also, the result showed significant differences in Alkaline phosphatase enzymes, serum glutamic oxaloacetic transaminase, Serum glutamic-pyruvic transaminase, between the test and control groups(p<0.05) while non-significant elevation of blood urea nitrogen, creatinine and total protein levels was found in the Celmanax®-received groups (p>0.05). These results suggest that the Celmanax® supplementation enhances white blood cells and neutrophil count, and changes some biochemical parameters in rainbow trout.
Article Details
  • Section
  • Research Articles
Download data is not yet available.
AdelM, Yeganeh S,Dadar M,Sakai M,Dawood MOA(2016) Effects of dietary Spirulina platensis on growth performance, humoral and mucosal immune responses and disease resistance in juvenile great sturgeon (HusohusoLinnaeus, 1754). Journal of Fish & Shellfish Immunology56:436-444.
Ahmdifar E,Akrami R,Ghelichi A,MohammadiZarejabadA (2011) Effects of different dietary prebiotic inulin levels on blood serum enzymes, hematologic, and biochemical parameters of great sturgeon (Husohuso) juveniles. Journal of Comparative Clinical Pathology20:447-451.
Akrami R,Razeghi Mansour M,Ghobadi SH,Ahmadifar E, Shaker Khoshroudi M,Moghimi Haji S (2013) Effect of prebiotic mannan oligosaccharide on haematological and blood serum biochemical parameters of cultured juvenile great sturgeon (Husohuso Linnaeus, 1754). Journal of Applied Ichthyology 29: 1274-1278.
Amani Denji K,Razeghi Mansour M, Akrami R,GhobadiSh,Jafarpour SA,MirbeygiSK (2015) Effect of Dietary Prebiotic Mannan Oligosaccharide (MOS) on Growth Performance, Intestinal Microflora, Body Composition, Haematological and Blood Serum Biochemical Parameters of Rainbow Trout (Oncorhynchus mykiss) Juveniles. Journal of Fisheries and Aquatic Science 10: 255-265.
AndrewsSR,Sahu NP, Pal AK, Kumar S (2009). Haematological modulation and growth of LabeoRohitafingerlings: Effect of Dietary Mannan Oligosaccharide, Yeast Extract, Protein Hydrolysate and Chlorella. J. Aquaculture Research 41: 61-69.
Anguiano M,PohlenzC, BuentelloA, GatlinDM (2013) The effects of prebiotics on the digestive enzymes and gut histomorphology of red drum (Sciaenopsocellatus) and hybrid striped bass (Moronechrysops× M. saxatilis). J. British Journal of Nutrition 109:623-629.
Barger AM, MacNeillAL (2015)Clinical Chemistry.In: Clinical Pathology and Laboratory Techniques for Veterinary Technicians. 1sted,Wiley Blackwell press, United Kingdom: Pp.123-128.
Dimitroglou A, Merrifield DL,Moate R, Davies SJ, Spring P,Sweetman J, Bradley G (2009) Dietary Mannan oligosaccharides supplementation modulates intestinal microbial ecology and improves morphology of rainbow trout, Oncorhynchus mykiss (Walbaum). J. Animal Science87: 3226–3234.
Dimitroglou A, MerrifieldDL, Spring P,Sweetman J,MoateRdaviesSJ (2010) Effects of mannan oligosaccharide (MOS) supplementation on growth performance, feed utilisation, intestinal histology and gut microbiota of gilthead sea bream (Sparusaurata). J. Aquaculture 300:182–188.
Dimitroglou A, MerrifieldDL,Carnevali O,Picchietti S, Avella M, DanielsC,Güroy D, Davies SJ (2011a) Microbial manipulations to improve fish health and production: A Mediterranean perspective. J. Fish and Shellfish Immunology30:1–16.
Dimitroglou A, Reynolds P,Ravnoy B, Johnsen F,Sweetman JW, Johansen J,DaviesSJ (2011b) The effect of mannan oligosaccharide supplementation on Atlantic salmon smolts (Salmo salarL.) fed diets with high levels of plant proteins. J. Aquaculture Research and Development1:1-11.
DimitroglouA,Moate R,Janssens T, Spring P,Sweetman JW, Davies SJ (2011c)Field observations on the effect of a mannan oligosaccharide on mortality and intestinal integrity of sole (Soleasenegalensis, Kaup) infected by Photobacteriumdamselaesubsp. piscicida. J. Aquaculture Research and DevelopmentS1:1-13.
Genç MA,Aktas M,Genc E, Yilmaz E (2007) Effects of dietary mannan oligosaccharide on growth, body composition and hepatopancreas histology of Penaeussemisulcatus(de Haan 1844). J. Aquaculture Nutrition13:156–161.
Grisdale-Helland B,Helland SJ,GatlinIIIDM (2008)The effects of dietary supplementation with mannan oligosaccharide, fructooligosaccharide or galactooligosaccharide on the growth and feed utilization of Atlantic salmon (Salmo salar). J. Aquaculture 283:163-167.
Gultepe N, Salnur S,Hossu B,HisarO(2011) Dietary supplementation with Mannanoligosaccharides (MOS) from Bio-Mos enhances growth parameters and digestive capacity of gilthead sea bream (Sparusaurata). J. Aquaculcher Nutrition 17:482-487.
Gültepe N,Kesbiç OS,Acar Ü,Gökkuş K,Gültepe MI,Sönmez AY, Bilen S, Aydın S, 2015. Effects of Prebiotic Mannan oligosaccharides (MOS) on Histology and Biochemical Blood Parameters of Gilthead Seabream, Sparusaurata. J. Aquaculture – Bamidgeh, 67,6-11.
Hisano H, Barros MM,PezzatoLE (2007)Leveduraezincocomo pro-nutrientesemracoes
para tilapia-do-Nilo (Oreochromisniloticus). J. AspectosHematologicosBoletim do Instituto de Pesca 33:35-42.
Hoseinifar SH,Mirvaghefi A, Merrifield DL,Amiri BM, Yelghi S,BastamiKD (2011) The study of some haematological and serum biochemical parameters of juvenile beluga (Husohuso) fed oligofructose. J. Fish Physiology 37:91-96.
Jalali MA,Ahmadifar E,Sudagar M, Azari TakamiG (2009) Growth efficiency, body composition, survival and haematological changes in great sturgeon (HusohusoLinnaeus, 1758) juveniles fed diets supplemented with different levels of Ergosan.J. Aquaculture Research40:804-809.
Marzouk MS,Moustafa MM, Mohamed NM (2008) Evaluation of immune modulatory effects of some probiotics on cultured Oreochoromisniloticus. In: 8th international symposium on tilapia in aquaculture, Cairo, Egypt :pp1043-1058.
Merrifield DL,Dimitroglou A,Foey A, Davies SJ, Baker RR,Bøgwald J,Castex M,RingøE (2010) The current status and future focus of probiotic and prebiotic applications for salmonids. J. Aquaculture302:1–18.
Merrifield DL,RingøE (2014)Prebiotics in Finfish: An Updatein: Aquaculture nutrition: gut health, probiotics, and prebiotics. 1sted. Wiley Blackwell Press, United Kingdom: PP 360-401.
Momeni-Moghaddam P, Keyvanshokooh S,Ziaei-NejadS, ParvizSalati A,ZanoosiPasha- H, (2015) Effects of mannan oligosaccharide supplementation on growth, some immune responses and gut lactic acid bacteria of common carp (CyprinusCarpio) fingerlings. Journal of Veterinary Research Forum 6:239–244.
Parisi G,Terova G,Gasco L, Piccolo G,Roncarati A, Moretti VM,CentoducatiG,Gatta PP,PaisA (2014) Current status and future perspectives of Italian finis aquaculture. Journal Reviews in Fish Biology and Fisheries 24:15-73.
Pryor GS,Royes JB, Chapman FA, Miles RD (2003)Mannan oligosaccharides in fish nutrition: effects of dietary supplementation on growth and gastrointestinal villi structure in Gulf of Mexico sturgeon. Journal North American Journal of Aquaculture 65(2):106–111.
Razeghi Mansour M,Akrami R,Ghobadi S, Amani Denji K,Ezatrahimi N,GharaeiA (2012) Effect of dietary mannan oligosaccharide (MOS) on growth performance, survival, body composition, and some hematological parameters in giant sturgeon juvenile (HusohusoLinnaeus, 1754). Journal of Fish Physiology and Biochemistry38(3):829–835.
Rehulka J,Minarik B,CinkD,ZalakJ (2011) Prebiotic effect of fructooligosaccharides on growth and physiological state of rainbow trout, Oncorhynchus mykiss (Walbaum). Journal ActaUniversitatisAgriculturaeEtSilviculturareMendelianaeBrunensis 59(1): 227–236.
Ringø E, Olsen RE,Gifstad TØ,Dalmo RA,Amlund H,Hemre GI,BakkeAM (2010) Prebiotics in aquaculture: a review. Journal Aquaculture Nutrition16:117–136.
Rodriguez-Estrada U, Satoh S,Haga Y, Fushimi H,Sweetman J (2009) Effects of single and combined supplementation of Enterococcus faecalis, mannan oligosaccharide and polyhydrobutyric acid on growth performance and immune response of rainbow trout (Oncorhynchus mykiss). Journal Aquaculture Science 57:609–617.
Roozbahani MR, Bandehpour M, Haghighi-Khiabanian-Asl A, Abdollahi H,KazemiB (2009) PCR-based detection of Yersinia ruckeri infection in rainbow trout fish. Asian Journal of Animal and Veterinary Advances 4(5):258-262.
Sado RY,Bicudo AJD,CyrinoJPE(2008) Feeding dietary mannan oligosaccharides to juvenile Nile tilapia, Oreochromisniloticus, has no effect on hematological parameters and showed decreased feed consumption. Journal of World Aquaculture Society 39(6):821-826.
Staykov Y, SpringP,Denev S,SweetmanJ (2007). Effect of a mannan oligosaccharide on the growth performance and immune status of rainbow trout (Oncorhynchus mykiss). Journal Aquaculture International 15(3):153-161.
Sweetman JW,Torrecillas S,Dimitroglou A, Rider S, Davies SJ,IzquierdoMS (2010) Enhancing the natural defences and barrier protection of aquaculture species. Journal Aquaculture Research 41(1):345–355.
TalpurAD,Munir MB,Mary A,HashimR (2014) Dietary probiotics and prebiotics improved food acceptability, growth performance, haematology and immunological parameters and disease resistance against Aeromonashydrophila in snakehead (Channastriata) fingerlings. Aquaculture Journal 427:14-20.
TanB, MaiK (2001) Zinc methionine and zinc sulfate as sources of dietary zinc for juvenile abalone, HaliotisdiscushannaiIno. Aquaculture Journal 192:67-84.
Thrall MA, Weiser G, Allison RW, Campbell TW (2012)Hematology of Fish. In: Veterinary Hematology and Clinical Chemistry, 2nd ed, Wiley Blackwell Press,United Kingdom: Pp298-312.
Tiengtam N, Paengkoum P, Sirivoharn S,PhonsiriK (2017) The effects of dietary inulin and Jerusalem artichoke (Helianthus tuberosus) tuber on the growth performance, haematological, blood chemical and immune parameters of Nile tilapia (Oreochromisniloticus) fingerlings. Journal Aquaculture Research 48(10):5280–5288.
TorrecillasS,Makol A, Caballero MJ, Montero D,RobainaL (2007) Immune stimulation and improved infection resistance in European sea bass (Dicentrarchuslabrax) fed mannan oligosaccharides. Journal of Fish and Shellfish Immunology 23(5):969-981.
Tukmechi A, Rahmati Andani HR,Manaffar R,SheikhzadehN (2011) Dietary administration of beta-mercapto-ethanol treated Saccharomyces cerevisiae enhanced the growth, innate immune response and disease resistance of the rainbow trout, Oncorhynchus mykiss. Journal of Fish and Shellfish Immunology 30(3):923–928.
Welker TL, Lim C,Yildirim-Aksoy M, Shelby R,KlesiusPH (2007) Immune response and resistance to stress and Edwardsiellaictalurichallenge in channel catfish, Ictalurus punctatus, fed diets containing commercial whole-cell yeast or yeast subcomponents. World Aquaculture Society Journal 38(1):24-35.
Wong ND, CupplesLA, Ostfeld AM, Levy D,KannelWB (1989) Risk factors for long-term coronary prognosis after initial myocardial infarction: the Framingham Study. American Journal of Epidemiology 130(3):469-480.
Ye JD, Wang K, Li FD, SunYZ (2011) Single or combined effects of fructo- and mannan oligosaccharide supplements and Bacillus clausiion the growth, feed utilization, body composition, digestive enzyme activity, innate immune response and lipid metabolism of the Japanese flounder paralichthysolivaceus. Journal of Aquaculture Nutrition 17(4):902-911.
Zorriehzahra MJ, Hassan MD, Gholizadeh M, Saidi AA (2010) Study of some haematological and biochemical parameters of Rainbow trout (Oncorhynchus mykiss) fry in western part of Mazandaran province, Iran. Iranian Journal of Fisheries Sciences9 (1):185-198.