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The healing effect of bone marrow-derived stem cells and aquatic activity in Achilles tendon injury

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H. SHEIKHANI-SHAHIN, D. MEHRABANI, M. J. ASHRAF, H. RAJABI, M. NOROUZIAN, F. RAHMANIFAR, S. DEHGHANI NAZHVANI, S. ZARE
H. SHEIKHANI-SHAHIN, D. MEHRABANI, M. J. ASHRAF, H. RAJABI, M. NOROUZIAN, F. RAHMANIFAR, S. DEHGHANI NAZHVANI, S. ZARE

Abstract


The common treatment recommended for Achilles tendon rupture as the most common tendon injury during exercise is surgical intervention, while it eventually causes various clinical problems. This study assessed the healing effect of bone marrow-derived stem cells (BMSCs) and aquatic activities in Achilles tendon injury. Forty rats were randomly divided into 5 equal groups. Group 1 underwent aquatic activity, 72 h after a crush lesion formed on Achilles tendon, group 2 received 1×106 intra-articular BMSCs post-tendon injury, group 3 had aquatic activity together with BMSCs transplantation after tendon damage, group 4 just experienced tendon injury without any treatment intervention and group 5 was considered as the control group while did not undergo any tendon injury and did not receive any treatment measure. After 8 weeks, the animals were sacrificed and the tendons were transferred in 10% formalin for histological evaluation. There was a significant increase in fibroblast number in group 3 in comparison to other groups. However, there was a significant increase in collagen deposition in groups 2, 3 and 5 in comparison to group 1 and 4. A significant decrease was noted for cellularity in group 2 when compared to groups 1 and 4. Regarding tendon diameter in group 3; a significant healing was observed when compared to groups 2, 4 and 5. It was shown that aquatic activity together with cell transplantation was an effective therapeutic measure enhancing the healing in tendon injuries. These findings can open a window in sport medicine in treatment of tendon injuries.


Keywords


Aquatic activity; Bone marrow; Mesenchymal stem cells; Healing; Tendon

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Aliborzi G, Vahdati A, Hossini SE, Mehrabani D (2015) Evaluation of bone marrow-derived mesenchymal stem cells from Guinea pigs. Onl J Vet Res 19:450-459.

Akinbo S, Aiyegbusi A, Duru F, Noronha C, Okanlawon A (2008) The efficacy of uktrasound therapy on the acute tendon injury. Internet J Orthop Surg 13:1-5.

Chong AK, Ang AD, Goh JC, Hui JH, Lim AY, Lee EH, Lim BH (2007) Bone marrow-derived mesenchymal stem cells influence early tendon-healing in a rabbit Achilles tendon model. Bone Joint Surg Am 89:74-81.

Curwin SL, Vailas AC, Wood J (1988) Immature tendon adaptation to strenuous exercise. J Appl Physiol 65:2297-2301.

Faramarzi H, Mehrabani D, Fard M, Akhavan M, Zare S, BakhshalizadehSh, Manafi A, Kazemnejad S, Shirazi R (2016) The potential of menstrual blood-derived stem cells in differentiation to epidermal lineage: a preliminary report. World J Plast Surg 5:26-31.

Gashmardi N, Hosseini SE, Mehrabani D, Edalatmanesh MA, Khodabandeh Z (2017) Impacts of bone marrow stem cells on caspase-3 levels after spinal cord injury in mice. Iran J Med Sci 42:593-598.

Godwin EE, Young NJ, Dudhia J, Beamish IC, Smith RK (2012) Implantation of bone marrow-derived mesenchymal stem cells demonstrates improved outcome in horses with overstrain injury of the superficial digital flexor tendon. Equine Vet J 44:25-32.

Harris MT, Butler DL, Boivin GP (2004) Mesenchymal stem cells used for rabbit tendon repair can form ectopic bone and express alkaline phosphatase activity in constructs. J Orthop Res 22:998-1003.

Hart KJ, Shaw JM, Vajda E, Hegsted M, Miller SC (2001) Swim-trained rats have greater bone mass, density, strength, and dynamics. J Appl Physiol 91:1663-1668.

He M, Gan AW, Lim AY, Goh JC, Hui JH, Chong AK (2015) Bone marrow derived mesenchymal stem cell augmentation of rabbit flexor tendon healing. Hand Surg 20:421-429.

Heinemeier KM (2007) Adaptation of tendon and muscle connective tissue to mechanical loading. Ph.D. thesis.

Hosseinkhani M, Mehrabani D, Karimfar MH, Bakhtiyari S, Manafi A, Shirazi R (2014) Tissue engineered scaffolds in regenerative medicine. World J Plast Surg 3:3-7.

Kjaer M (2004) Role of extracellular matrix in adaptation of tendon and skeletal muscle to mechanical loading. Physiol Rev 84:649-698.

Lacitignola L, Crovace A, Rossi G, Francioso E (2008) Cell therapy for tendinitis, experimental and clinical report. Vet Res Commun 32:S33-38.

Lane JG, Healey RM, Chase DC, Amiel D (2013) Use of platelet-rich plasma to enhance tendon function and cellularity. Am J Orthop 42:209-214.

Lisa M, Zena W (2002) Inflammation and cancer. Nature 420:860-867.

McGoldrick R, Chattopadhyay A, Crowe C, Chiou G, Hui K, Farnebo S, Davis C, Le Grand A, Jacobs M, Pham H, Chang J (2017) The tissue engineered tendon bone interface: In Vitro and In Vivo synergistic effects of adipo-derived stem cells, platelet rich plasma and extracellular matrix hydrogel. Plast Reconstr Surg 140:1169-1184.

McVeigh J, Kingsley S, Gray D, Loram LC (2010) Swimming enhances bone mass acquisition in growing female rats. J Sports Sci Med 9:612-619.

Lui PP, Wong OT, Lee YW (2016) Transplantation of tendon-derived stem cells pre-treated with connective tissue growth factor and ascorbic acid in vitro promoted better tendon repair in a patellar tendon window injury rat model. Cytotherapy 18:99-112.

Mehrabani D, Hassanshahi MA, Tamadon A, Zare S, Keshavarz S, Rahmanifar F, Dianatpour M, Khodabandeh Z, Jahromi I, Tanideh N, Ramzi M, Aqababa H, Kuhi-Hoseinabadi O (2015a) Adipose tissue-derived mesenchymal stem cells repair germinal cells of seminiferous tubules of busulfan-induced azoospermic rats. J Hum

Reprod Sci 8:103-110.

Mehrabani D, Mahdiyar P, Torabi K, Robati R, Zare S, Dianatpour M, Tamadon A (2017) Growth kinetics and characterization of human dental pulp stem cells: Comparison between third molar and first premolar teeth. J Clin Exp Dent 9:e172-e177.

Mehrabani D, Mehrabani G, Zare S, Manafi A (2013) Adipose-derived stem cells ADSC) and aesthetic surgery: a mini review. World J Plast Surg 2: 65-70.

Mehrabani D, Rahmanifar F, Mellinizhad M, Tamadon A, Dianatpour M, Zare S, RazeghianJahromi I, Ghobadi F (2015b) Isolation, culture, characterization, and adipogenic differentiation of heifer endometrial mesenchymal stem cells. Comp Clin Pathol 24:1159-64.

Michna H, Hartmann G (1989) Adaptation of tendon collagen to exercise. Int Orthop 13:161-165.

Park GY, Kwon DR, Lee SC (2015) Regeneration of full-thickness rotator cuff tendon tear after ultrasound-guided injection with umbilical cord blood-derived mesenchymal stem cells in a rabbit model. Stem Cells Transl Med 4:1344-1351.

Rahmanifar F, Tamadon A, Mehrabani D, Zare S, Abasi S, Keshavarz S, Dianatpour M, Khodabandeh Z, Jahromi IR, Koohi-Hoseinabadi O (2016) Histomorphometric evaluation of treatment of rat azoospermic seminiferous tubules by allotransplantation of bone marrow-derived mesenchymal stem cells. Iran J Basic Med Sci 19:653-661.

Rajabi H, Sheikhani Shahin H, Norouzian M, Mehrabani D, Dehghani Nazhvani S (2015) The healing effects of aquatic activities and allogenic injection of platelet-rich plasma (PRP) on injuries of Achilles tendon in experimental rat. World J Plast Surg 4:66-73.

RazeghianJahromi I, Mehrabani D, Mohammadi A, Dianatpour M, Tamadon A, Zare S, Ghahremani Seno M, Khodabandeh Z (2016) The effect of fetal rat brain extract on morphology of bone marrow-derived mesenchymal stem cells. Comp Clin Pathol 25: 343–349.

Rodeo SA, Delos D, Weber A (2010) What’s new in orthopaedic research. J Bone Joint Surg Am 92:2491-2501.

Romero A, Barrachina L, Ranera B, Remacha AR, Moreno B, de Blas I, Sanz A, Vázquez FJ, Vitoria A, Junquera C, Zaragoza P, Rodellar C (2017) Comparison of autologous bone marrow and adipose tissue derived mesenchymal stem cells, and platelet rich plasma, for treating surgically induced lesions of the equine superficial digital flexor tendon. Vet J 224:76-84.

Shamosi A, Mehrabani D, Azami M, Ebrahimi-Barough S, Siavashi V, Ghanbari H, Sharifi E, Roozafzoon R, Ai J (2017) Differentiation of human endometrial stem cells into endothelial-like cells on gelatin/chitosan/bioglassnanofibrous scaffolds. Artif Cells Nanomed Biotechnol 45:163-173.

ShaterzadehYazdi H, Mehrabani D, KhodakaramTafti A, Dianatpour M, Zare S, Tamaddon A, RazeghianJahromi I (2015) Osteogenic potential of subcutaneous adipose-derived stem cells in a rabbit model. Onl J Vet Res 19:436-445.

Vuornos K, Björninen M, Talvitie E, Paakinaho K, Kellomäki M, Huhtala H, Miettinen S, Seppänen-Kaijansinkko R, Haimi S (2016) Human adipose stem cells differentiated on braided polylactide scaffolds is a potential approach for tendon tissue engineering. Tissue Eng Part A 22:513-523.

Wang JHC (2006) Mechanobiology of tendon. J Biomech 39:1563-1582.


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Copyright (c) 2019 H. SHEIKHANI-SHAHIN, D. MEHRABANI, M. J. ASHRAF, H. RAJABI, M. NOROUZIAN, F. RAHMANIFAR, S. DEHGHANI NAZHVANI, S. ZARE

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