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An improved cervical mucus penetration test

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A simple and rapid procedure for following the migration of spermatozoa in bovine cervical mucus, incorporating some of the advantages of the «coverslip» and «capillary tube» methods, is described. The method measures reproducibly the following parameters: penetration depth, density, degree and duration of motility, and it permits, at the same time, a morphological examination of the spermatozoa. Using this method it was found that at 37°C bovine spermatozoa migrate in the cervical mucus of the oestrus cow at a constant rate, with a mean velocity of 58.0 ± 1.25μΑεο. The sperm migration rate at 30 C, and even more so at 37°C, was higher than at 22°C. There was a good correlation between velocity registered after 5,10 and 15 min. The coefficient of variation ranged from 2.7 to 21.9; higher values were recorded in samples with low rate of sperm penetration. Individual variability between samples of semen and cervical mucus was evident. Freezing and storing of cervical mucus at -30' C for one month did not alter the results of the penetration test. Dilution of semen of good motility to 30 X 107ml did not affect the readings.


Bancorft, Η., (1957). Introduction to Biostatistics, Hoeber-Harper, New York.

Baker, F.N., Cragle, R.G., Salisbury, G.W and Vandemark, N.L., (1957). Spermatozoan velocities in vitro. Fertil. Steril. 8: 149-155.

Beck, K.J., (1971). Cervikale Factoren als Ursache der Sterilität. Gynekologia (Basel) 33: 141-147.

Berner, D. and Krell, L., (1970). Der in vitro Penetrationtest, eine zusätzliche Untersuchungsmethode in der Fertilitätsdiagnostik. Derm. Mschr. 156: 46-51.

Botella-Llusia, J., (1956). Measurment of linear progression of the human spermatozoa as an index of male fertility. Int. J. Fertil. 1: 113-130.

Carlborg, L., (1969). Determination of sperm migration rate in small samples of cervical mucus. Acta Endocr. Copench. 62: 732-746.

Guard, H.R., (1960). New technic for sperm-mucus penetration tests using a hemocytometer. Fert. Steril. 11: 392-398.

Hagen, Η., (1968). Zur Verbesserung der Diagnostic bei fuctioneller Sterilitaät durch einen Sperma-Aszensiontest. Z. Gynaek. 90: 1181-1184.

Kesserü, E. and Larranaga, Α., (1971). In vitro sperm migration in the human cervical mucus with different contraceptive methods. Contraception, 3: 195-208.

Kremer, J., (1965). A simple sperm penetration test. Inter. J. Fertil. 10: 209-215.

Kurzrok, R. and Miller, E.G., (1928). Biochemical studies of human semen and its relationship to mucus of the cervix uteri. Amer. J. Obstet. Gynec, 15: 56-72.

Kurzrok. R. and Miller, E.G., (1932). Biochemical studies of human semen. III. Factors affecting migration of sperm through the cervix. Amer. J. Obstet. Gyn., 24: 19.

Lamar, J., Shettles, L. and Delfs, E., (1940). Cyclic penetrability of human cervical mucus to spermatozoa in vitro. Amer. J. Physiol, 129: 234-241.

Moberg, R., (1969). Changes in cervical mucus smears from ovariectomized heifers treated with sexual hormones. XVIth Int. Vet. Congr., Madrid, Bd. 11: 937-938.

Moghissi, K.S., (1972). The function of the cervix in fertility. Fertil. Steril., 23: 295-306.

Odeblad, E., (1969). Types of human cervical mucus secrition. Acta Europ. Fertil. 1: 99-116.

Reichman, J., Insler, V. and Serr, D., (1973). A modified in vitro spermatozoa penetration test. Int. J. Fertil, 18: 232-240.

Rothschild, L., (1953). A new method of measuring sperm speeds. Nature, 171: 512-513.

Schwartz, R. and Zinnser, H.H., (1955). Some factors modifying sperm progression. Fertil. Steril., 6: 450-458.

Tampion, D. and Gibbons, R. Α., (1962). Swimming rate of bull sperm. Nature, 194: 695.

Tampion, D. and Gibbons, R.A., (1963). Swimming rate of bull spermatozoa in various media and the effect of dilution. J. Reprod. Fertil, 5: 259-275.

Veser, Α., (1969). Über physikalische, chemische und biologische Eigenschaften der Zervikalsekrete von Rindern, Vet. med. Diss., München.


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