Red wine polyphenols modulate bone loss in the ovariectomized rat model of postmenopausal osteoporosis

PDF
DOI: https://doi.org/10.12681/jhvms.21749
Keywords:
Rats Osteoporosis Polyphenols Bone mineral density Three point bending
C. PASSALI
Laboratory for the Research of the Musculoskeletal System, School of Medicine, National & Kapodistrian University of Athens, Greece
A. PATSAKI
Laboratory for the Research of the Musculoskeletal System, School of Medicine, National & Kapodistrian University of Athens, Greece
P. LELOVAS
Laboratory for the Research of the Musculoskeletal System, School of Medicine, National & Kapodistrian University of Athens, Greece
N. ALIGIANNIS
Department of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, National & Kapodistrian University of Athens, Greece
M. MAKROPOULOU
Department of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, National & Kapodistrian University of Athens, Greece
S. KOURKOULIS
Unit of Biomechanics, Department of Mechanics, School of Applied Mathematical and Physical Sciences, National Technical University of Athens, Greece
N. PAPAIOANNOU
Laboratory for the Research of the Musculoskeletal System, School of Medicine, National & Kapodistrian University of Athens, Greece
S. MITAKOU
Department of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, National & Kapodistrian University of Athens, Greece
A.L. SKALTSOUNIS
Department of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, National & Kapodistrian University of Athens, Greece
I. DONTAS
Laboratory for the Research of the Musculoskeletal System, School of Medicine, National & Kapodistrian University of Athens, Greece
Abstract

The aim of this study was to examine the effect of Red Wine Polyphenols (RWPs) extract on bone mineral density (BMD) in the ovariectomized (OVX) rat model. Thirty-five 10-month-old Wistar rats were separated into controls (CTRL), OVX and OVX plus RWPs in their drinking water (dose, 50 mg/kg body weight per day), starting immediately after OVX for 6 months. Βody and uterine weight, BMD of the tibia at baseline, 3 and 6 months post-OVX, and 3-pointing bending of the femur, were examined. Statistical comparison of the total tibia BMD within groups during the study period showed a significant reduction in the OVX and OVX+RWPs groups both from baseline to 3 and 6 months and from 3 to 6 months, whereas in the CTRL group, there was no significant change. For the proximal tibial metaphysis, comparison of BMD percentage changes from baseline to 3 months and 6 months and from 3 to 6 months revealed highly statistical differences between OVX and OVX+RWPs groups (P = 0.000). OVX induced a significant reduction of biomechanical parameters as expected; the 3-point bending test showed that the maximum force before fracture, energy absorption and fracture stress significantly increased in the OVX group treated with RWPs compared with the nontreated OVX rats (P = 0.048, P = 0.002 and P = 0.003, respectively). Dietary intake of RWPs for 6 months significantly prevented trabecular bone loss and improved bone strength in estrogen-deficient ovariectomized rats.

Article Details
  • Section
  • Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
Download data is not yet available.
References
Agarwal, C., Sharma, Y., Zhao, J., & Agarwal, R. (2000). A polyphenolic fraction from grape seeds causes irreversible growth inhibition of breast carcinoma MDA-MB468 cells by inhibiting mitogen-activated protein kinases activation and inducing G1 arrest and differentiation. Clinical Cancer Research: An Official Journal of the American Association for Cancer Research, 6(7), 2921–2930.
Waynforth, B. H., & Flecknell, P. A. (1992). Experimental and Surgical Technique in the Rat. London : Academic Press.
Canalis, E., McCarthy, T., & Centrella, M. (1988). Growth factors and the regulation of bone remodeling. The Journal of Clinical Investigation, 81(2), 277–281.
Cao, P.-C., Xiao, W.-X., Yan, Y.-B., Zhao, X., Liu, S., Feng, J., Lei, W. (2014). Preventive Effect of Crocin on Osteoporosis in an Ovariectomized Rat Model. Evidence-Based Complementary and Alternative Medicine: eCAM, 2014, 1–11.
De França, N. A. G., Camargo, M. B. R., Lazaretti-Castro, M., & Martini, L. A. (2013). Antioxidant intake and bone status in a cross-sectional study of Brazilian women with osteoporosis. Nutrition and Health, 22(2), 133–142.
Dontas, I. Α., Lelovas, P. P., Kourkoulis, S. K., Aligiannis, N., Paliogianni, A., Mitakou, S., Galanos, A., Kassi, E., Mitousoudis, A., Xanthos, T., Papaioannou, N., Lyritis, G. P. (2011). Protective effect of Sideritis euboea extract on bone mineral density and strength of ovariectomized rats. Menopause , 18(8), 915–922.
Dontas, I., Halabalaki, M., Moutsatsou, P., Mitakou, S., Papoutsi, Z., Khaldi, L., Galanos, A., Lyritis, G. P. (2006). Protective effect of plant extract from Onobrychis ebenoides on ovariectomy-induced bone loss in rats. Maturitas, 53(2), 234–242.
Eng, E. T., Ye, J., Williams, D., Phung, S., Moore, R. E., Young, M. K., … Chen, S. (2003). Suppression of estrogen biosynthesis by procyanidin dimers in red wine and grape seeds. Cancer Research, 63(23), 8516–8522.
Ferguson, N. (2004). Osteoporosis in focus. Pharmaceutical Press.
Hohman, E. E., & Weaver, C. M. (2015). A Grape-Enriched Diet Increases Bone Calcium Retention and Cortical Bone Properties in Ovariectomized Rats. The Journal of Nutrition, 145(2), 253–259.
Jee, W. S., & Yao, W. (2001). Overview: animal models of osteopenia and osteoporosis. Journal of Musculoskeletal & Neuronal Interactions, 1(3), 193–207.
Lelovas, P. P., Xanthos, T. T., Thoma, S. E., Lyritis, G. P., & Dontas, I. A. (2008). The laboratory rat as an animal model for osteoporosis research. Comparative Medicine, 58(5), 424–430.
L’Hermite, M. (2017). Custom-compounded bioidentical hormone therapy: why so popular despite potential harm? The case against routine use. Climacteric: The Journal of the International Menopause Society, 20(3), 205–211.
Lloret, A., Coiffier, G., Couchouron, T., Perdriger, A., & Guggenbuhl, P. (2016). Risk factors of mortality during the first year after low energy osteoporosis fracture: a retrospective case-control study. Clinical Cases in Mineral and Bone Metabolism: The Official Journal of the Italian Society of Osteoporosis, Mineral Metabolism, and Skeletal Diseases, 13(2), 123–126.
Miller, V. M., Lahr, B. D., Bailey, K. R., Heit, J. A., Harman, S. M., & Jayachandran, M. (2016). Longitudinal effects of menopausal hormone treatments on platelet characteristics and cell-derived microvesicles. Platelets, 27(1), 32–42.
Oster, P., Arab, L., Kohlmeier, M., Mordasini, R., Schellenberg, B., & Schlierf, G. (1982). Effects of estrogens and progestogens on lipid metabolism. American Journal of Obstetrics and Gynecology, 142(6 Pt 2), 773–775.
Patsaki, A., Tchoumtchoua, J., Passali, C., Lelovas, P., Kourkoulis, S., Papaioannou, N., Mbanya J-C., Njamen D., Mitakou S., Halabalaki M., Dontas, I. (2016). The Protective Effect of Amphimas pterocarpoides Plant Extract on Bone Mineral Density and Strength in Estrogen Deficient Ovariectomized Wistar Rats. Medicinal & Aromatic Plants, 5(5), 1–7.
Pawlowski, J. W., Martin, B. R., McCabe, G. P., Ferruzzi, M. G., & Weaver, C. M. (2014). Plum and soy aglycon extracts superior at increasing bone calcium retention in ovariectomized Sprague Dawley rats. Journal of Agricultural and Food Chemistry, 62(26), 6108–6117.
Ran, S. Y., Yu, Q., Chen, Y., & Lin, S. Q. (2017). Prevention of postmenopausal osteoporosis in Chinese women: a 5-year, double-blind, randomized, parallel placebo-controlled study. Climacteric: The Journal of the International Menopause Society, 20(4), 391–396.
Sharma, G., Tyagi, A. K., Singh, R. P., Chan, D. C. F., & Agarwal, R. (2004). Synergistic anti-cancer effects of grape seed extract and conventional cytotoxic agent doxorubicin against human breast carcinoma cells. Breast Cancer Research and Treatment, 85(1), 1–12.
Termine, J. D., & Wong, M. (1998). Post-menopausal women and osteoporosis: available choices for maintenance of skeletal health. Maturitas, 30(3), 241–245.
Thulkar, J., Singh, S., Sharma, S., & Thulkar, T. (2016). Preventable risk factors for osteoporosis in postmenopausal women: Systematic review and meta-analysis. Journal of Mid-Life Health, 7(3), 108–113.
Wang, T., Liu, Q., Tjhioe, W., Zhao, J., Lu, A., Zhang, G., Tan, R., Zhou, M., Feng, H. (2017). Therapeutic potential and outlook of alternative medicine for osteoporosis. Current Drug Targets.
Weisz, G. M. (2017). Osteoporosis: A Future Public Health Problem for Israel? Medical and Legal Obligations. The Israel Medical Association Journal: IMAJ, 19(4), 203–206.
Yahara, N., Tofani, I., Maki, K., Kojima, K., Kojima, Y., & Kimura, M. (2005). Mechanical assessment of effects of grape seed proanthocyanidins extract on tibial bone diaphysis in rats. Journal of Musculoskeletal & Neuronal Interactions, 5(2), 162–169.
Zhang, Z., Zheng, L., Zhao, Z., Shi, J., Wang, X., & Huang, J. (2014). Grape seed proanthocyanidins inhibit H2O2-induced osteoblastic MC3T3-E1 cell apoptosis via ameliorating H2O2-induced mitochondrial dysfunction. The Journal of Toxicological Sciences, 39(5), 803–813.
Most read articles by the same author(s)