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The in vitro toxicity of atrazine on kinematics and DNA fragmentation in common carp (Cyprinus carpio) sperm cells

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M.E. ÖZGÜR
M.E. ÖZGÜR

Abstract


This study investigated the in vitro effects of different concentrations of Atrazine (0.001, 0.01, 0.1, 0.5 mg/L) added to motile and immotile solutions on kinematics quality of sperm cells of common carp, Cyprinus carpio, which is a fish of economic significance. The kinematics of the sperm cells was analyzed by a computer-assisted sperm analysis system (CASA). As a result of the study, while there was a significant difference (P < 0.05) between the groups in terms of the VSL (μm/s) and VCL (μm/s) values after the Atrazine-added immotile solution’s (IMS) and incubation for 3 hours, there was a significant difference (P < 0.05) in only the VSL values directly activated by the Atrazine-added motile solution (MS). DNA fragmentation was evident but not in higher numbers in the 0.1 mg/L atrazine group. Finally, it was determined the effective concentration (EC50) values of the VSL value of the motile and immotile solution as 0.34 mg/L and 0.03 mg/L, respectively.


Keywords


Cyprinus carpio; sperm cells kinematics; DNA fragmentation; atrazine; reproduction

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References


Abascal FJ, Cosson J, Fauvel C (2007) Characterization of sperm motility in sea bass: The effect of heavy metals and physicochemical variables on sperm motility. J Fish Biol 70:509–522 . doi: 10.1111/j.1095-8649.2007.01322.x

Alavi SMH, Cosson J (2005) Sperm motility in fishes. I. Effects of temperature and pH: A review. Cell Biol Int 29:101–110 . doi: 10.1016/j.cellbi.2004.11.021

Alavi SMH, Cosson J (2006) Sperm motility in fishes. (II) Effects of ions and osmolality: A review. Cell Biol Int 30:1–14 . doi: 10.1016/j.cellbi.2005.06.004

Billard R, Cosson MP (1992) Some problems related to the assessment of sperm motility in freshwater fish. J Exp Zool 261:122–131 . doi:10.1002/jez.1402610203

Bozkurt Y, Yavaş İ (2017) Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm. J Limnol Freshw Fish Res 3:25–25 . doi: 10.17216/limnofish.277835

Dietrich GJ, Dietrich M, Kowalski RK, Dobosz S, Karol H, Demianowicz W, Glogowski J (2010) Exposure of rainbow trout milt to mercury and cadmium alters sperm motility parameters and reproductive success. Aquat Toxicol 97:277–284 . doi: 10.1016/j.aquatox.2009.12.010

Dzyuba V, Cosson J (2014) Motility of fish spermatozoa: From external signaling to flagella response. Reprod. Biol. Fabbrocini A, D’Adamo R, Del Prete F, Luca Langellotti A, Rinna F, Silvestri F, Sorrenti G, Vitiello V, Sansone G (2012) Cryopreserved semen in ecotoxicological bioassays: Sensitivity and reliability of cryopreserved Sparus aurata spermatozoa. Ecotoxicol Environ Saf 84:293–298 . doi: 10.1016/j.ecoenv.2012.07.024

Fauvel C, Suquet M, Cosson J (2010) Evaluation of fish sperm quality. J Appl Ichthyol 26:636–643 . doi: 10.1111/j.1439-0426.2010.01529.x

Graham N (1999) Guidelines for Drinking-Water Quality, 2nd edition, Addendum to Volume 1 – Recommendations, World Health Organisation, Geneva, 1998, 36 pages. Urban Water 1:183 . doi: 10.1016/S1462-0758(00)00006-6

Hatef A, Alavi SMH, Golshan M, Linhart O (2013) Toxicity of environmental contaminants to fish spermatozoa function in vitro-A review. Aquat Toxicol 140–141:134–144 . doi: 10.1016/j.aquatox.2013.05.016

Kime DE, Ebrahimi M, Nysten K, Roelants I, Rurangwa E, Moore HDM, Ollevier F (1996) Use of computer assisted sperm analysis (CASA) for monitoring the effects of pollution on sperm quality of fish; application to the effects of heavy metals. Aquat Toxicol 36:223–237 . doi:10.1016/S0166-445X(96)00806-5

Kime DE, Van Look KJW, McAllister BG, Huyskens G, Rurangwa E, Ollevier F (2001) Computer-assisted sperm analysis (CASA) as a tool for monitoring sperm quality in fish. Comp Biochem Physiol - C Toxicol Pharmacol 130:425–433 . doi: 10.1016/S1532-0456(01)00270-8

Li ZH, Li P, Dzyuba B, Randak T (2010a) Influence of environmental related concentrations of heavy metals on motility parameters and antioxidant responses in sturgeon sperm. Chem Biol Interact 188:473–477 . doi: 10.1016/j.cbi.2010.09.005

Li ZH, Li P, Rodina M, Randak T (2010b) Effect of human pharmaceutical Carbamazepine on the quality parameters and oxidative stress in common carp (Cyprinus carpio L.) spermatozoa. Chemosphere 80:530–534 . doi: 10.1016/j.chemosphere.2010.04.046

Poupard GP, Paxion C, Cosson J, Jeulin C, Fierville F, Billard R (1998) Initiation of carp spermatozoa motility and early ATP reduction after milt contamination by urine. Aquaculture 160:317–328 . doi:10.1016/S0044-8486(97)00301-3

Rurangwa E, Kime DE, Ollevier F, Nash JP (2004) The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture 234:1–28 . doi: 10.1016/j.aquaculture.2003.12.006

Shelley LK, Ross PS, Kennedy CJ (2012a) Immunotoxic and cytotoxic effects of atrazine, permethrin and piperonyl butoxide to rainbow trout following in vitro exposure. Fish Shellfish Immunol 33:455–458. . doi: 10.1016/j.fsi.2012.05.020

Shelley LK, Ross PS, Miller KM, Kaukinen KH, Kennedy CJ (2012b) Toxicity of atrazine and nonylphenol in juvenile rainbow trout (Oncorhynchus mykiss): Effects on general health, disease susceptibility and gene expression. Aquat Toxicol 124–125:217–226 . doi:10.1016/j.aquatox.2012.08.007

Shinn C, Santos MM, Lek S, Grenouillet G (2015) Behavioral response of juvenile rainbow trout exposed to an herbicide mixture. Ecotoxicol Environ Saf 112:15–21 . doi: 10.1016/j.ecoenv.2014.10.013

Toughan H, Khalil SR, El-Ghoneimy AA, Awad A, Seddek AS (2018) Effect of dietary supplementation with Spirulina platensis on Atrazine-induced oxidative stress- mediated hepatic damage and inflammation in the common carp (Cyprinus carpio L.). Ecotoxicol Environ Saf 149:135–142 . doi: 10.1016/j.ecoenv.2017.11.018

USEPA (2006) Decision Documents for Atrazine. In: Decision documents for atrazine

Van Look KJW, Kime DE (2003) Automated sperm morphology analysis in fishes: The effect of mercury on goldfish sperm. J Fish Biol 63:1020–1033 . doi: 10.1046/j.1095-8649.2003.00226.x

Wang X, Li J, Xing H, Xu S (2011) Review of Toxicology of Atrazine and Chlorpyrifos on Fish. J Northeast Agric Univ (English Ed 18:88–92. doi: 10.1016/S1006-8104(12)60031-2

Xing H, Zhang Z, Yao H, Liu T, Wang L, Xu S, Li S (2014) Effects of atrazine and chlorpyrifos on cytochrome P450 in common carp liver. Chemosphere 104:244–250 . doi: 10.1016/j.chemosphere.2014.01.002


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