Effect of a nanocomposite containing ostrich eggshell on calvarium healing in the rabbit: a pathologic study

PDF
DOI: https://doi.org/10.12681/jhvms.22218
Keywords:
Nano- composite Ostrich eggshell Calvarium autograft Rabbit
H ALEMI
Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
A. ASGHARI
Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
G. ABEDI
Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
A. AKBARZADEH
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Universal Scientific Education and Research Network (USERN), Tabriz, Iran
P. MORTAZAVI
Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract

The aim of the current study was to determine effect of a nanocomposite containing ostrich eggshell (NCOE) on the calvarium healing in the rabbit. Fresh ostrich eggshell was ground (300-500 μm), treated in phosphate-containing solutions and sterilized by gamma irradiation. Fifteen New Zealand white adult male rabbits were used. Four full-thickness skull defects were created in the calvarium. The first defect kept unfilled (control). The second defect was filled with autograft bone. The third defect was filled using NCOE. The fourth defect was filled with mixture of the autograft+NCOE bone. At 30, 60 and 90 days after surgery animals were euthanized and tissue specimens were collected and stained with hematoxylin eosin and trichrome staining method. Microsections were examined to

assess the extent and intensity of inflammation, calvarium formation status and foreign body reaction. According to the results, filling defect significantly increased in NCOE-treated rabbits compared to the control group at 30 and 60 days post-surgery (P<0.05). There a statistically significant difference between experimental groups compared to the control group at 30 and 60 days post-surgery (P<0.05) while no statistically significant differences were observed among autograft, NCOE, autograft+NCOE (P>0.05). Also, absorb material significantly decreased in NCOE and autograft+NCOE groups compared to the control group at 60 days post-surgery (P<0.05). The filling defect significantly increased in autograft, NCOE and NCOE+autograft groups compared to the control group at 90 days post-surgery (P<0.05). There was no significant difference on inflammation and absorb material among the groups at 90 days post-surgery (P>0.05). These results suggested NCOE+autograft has improved the rate of calvarium healing in rabbits.

Article Details
  • Section
  • Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
Download data is not yet available.
References
Abdulrahman I, Tijani HI, Mohammed BA, Saidu H, Yusuf H, Ndejiko Jibrin M, et al. From garbage to biomaterials: an overview on egg shell based hydroxyapatite. J Material 2014;2014.
Alemi H, Asghari A, Abedi G, Akbarzadeh A, Mortazavi P. The Effects of Nanocomposite Containing Ostrich Eggshell on Calvaria Bone Formation in Rabbit: Radiographic and Hematological Survey. Crescent J Med Biol Sci 5(2).
Baliga M, Davies P, Dupoirieux L. Powdered eggshell in the repair of cystic cavities of the jaw. Preliminary study. Rev Stomatol Chir Maxillofac 1998;99:86-8.
Barrere F, van der Valk CM, Dalmeijer RA, Meijer G, van Blitterswijk CA, de Groot K, et al. Osteogenecity of octacalcium phosphate coatings applied on porous metal implants. J Biomed Material ResPart A 2003;66(4):779-88.
Betoni-Junior W, Dechichi P, Esteves JC, Zanetta-Barbosa D, Magalhães AEO. Evaluation of the bone healing process utilizing platelet-rich plasma activated by thrombin and calcium chloride: a histologic study in rabbit calvaria. J Oral Implantol 2013;39(1):14-21.
Biazar E, Heidari Keshel S, Tavirani MR, Jahandideh R. Bone reconstruction in rat calvarial defects by chitosan/hydroxyapatite nanoparticles scaffold loaded with unrestricted somatic stem cells. Artificial cells Nanomed Biotechnol 2015;43(2):112-6.
Daculsi G, Laboux O, Malard O, Weiss P. Current state of the art of biphasic calcium phosphate bioceramics. J Material Sci Material Med 2003;14(3):195-200.
Dupoirieux L, Pourquier D, Neves M, Téot L. Resorption kinetics of eggshell: an in vivo study. J Craniofac Surg 2001;12(1):53-8.
Dupoirieux L, Pourquier D, Souyris F. Powdered eggshell: a pilot study on a new bone substitute for use in maxillofacial surgery. J Cranio-Maxillofacial Surg1995;23(3):187-94.
Durmuş E, Celik I, Aydın MF, Yıldırım G, Sur E. Evaluation of the biocompatibility and osteoproductive activity of ostrich eggshell powder in experimentally induced calvarial defects in rabbits. Journal of Biomedical Material Res Part B: Appl Biomaterial 2008;86(1):82-9.
Durmuş E, Celik I, Ozturk A, Ozkan Y, Aydin M. Evaluation of the potential beneficial effects of ostrich eggshell combined with eggshell membranes in healing of cranial defects in rabbits. J Int Med Res 2003;31(3):223-30.
Furlaneto FA, Nagata MJ, Fucini SE, Deliberador TM, Okamoto T, Messora MR. Bone healing in critical‐size defects treated with bioactive glass/calcium sulfate: a histologic and histometric study in rat calvaria. Clin Oral Implant Rres 2007;18(3):311-8.
Jones MD, Tran CW, Li G, Maksymowych WP, Zernicke RF, Doschak MR. In vivo microfocal computed tomography and micro-magnetic resonance imaging evaluation of antiresorptive and anti-inflammatory drugs as preventive treatments of osteoarthritis in the rat. Arthritis Rheumatol 2010;62(9):2726-35.
Huo MH, Troiano NW, Pelker RR, Gundberg CM, Friedlaender GE. The influence of ibuprofen on fracture repair: biomechanical, biochemical, histologic, and histomorphometric parameters in rats. J Orthop Res 1991; 9(3):383-90.
Jörundsson E, Lumsden JH, Jacobs RM. Rapid staining techniques in cytopathology: a review and comparison of modified protocols for hematoxylin and eosin, Papanicolaou and Romanowsky stains. Vet Clin Pathol. 1999; 28(3): 100-108.
Kattimani VS, Chakravarthi PS, Kanumuru NR, Subbarao VV, Sidharthan A, Kumar TS, et al. Eggshell derived hydroxyapatite as bone graft substitute in the healing of maxillary cystic bone defects: a preliminary report. Journal of international oral health: JIOH. 2014;6(3):15.
Krishna DSR, Siddharthan A, Seshadri S, Kumar TS. A novel route for synthesis of nanocrystalline hydroxyapatite from eggshell waste. J Mater Sci Mater Med 2007;18(9):1735-43.
Leung JY, Ho AY, Ip T, Lee G, Kung AW. The efficacy and tolerability of risedronate on bone mineral density and bone turnover markers in osteoporotic Chinese women: a randomized placebo-controlled study. Bone 2005;36(2):358-64.
Lim HC, Sohn JY, Park JC, Um YJ, Jung UW, Kim CS, et al. Osteoconductive effects of calcium phosphate glass cement grafts in rabbit calvarial defects. J Biomed Material Res Part B: Applied Biomaterial 2010;95(1):47-52.
Lindhe J, Nyman S, Ericsson I, Lindhe J, Lang N, Karring T. Trauma from occlusion: Periodontal tissues. Lindhe J, Lang NP, Karring T. Clinical Periodontology and Implant Dentistry 5th ed Oxford: Blackwell Munksgaard. 2008:363-73.
Marx RE. Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg 2004;62(4):489-96.
Neunzehn J, Szuwart T, Wiesmann H-P. Eggshells as natural calcium carbonate source in combination with hyaluronan as beneficial additives for bone graft materials, an in vitro study. Head Face Med 2015;11(1):12.
Paknejad M, Rokn A, Rouzmeh N, Heidari M, Titidej A, Kharazifard MJ,et al. Histologic evaluation of bone healing capacity following application of inorganic bovine bone and a new allograft material in rabbit calvaria. J Dent (Tehran, Iran). 2015;12(1):31.
Park JW, Bae SR, Suh JY, Lee DH, Kim SH, Kim H, et al. Evaluation ofbone healing with eggshell‐derived bone graft substitutes in rat calvaria: A pilot study. J Biomed Material Res Part A 2008;87(1):203-14.
Pastoureau P, Hunziker EB, Pelletier J-P. Cartilage, bone and synovial histomorphometry in animal models of osteoarthritis. Osteoarthritis Cartilage 2010;18:S106-S12.
Permuy M, Guede D, López-Peña M, Muñoz F, González-Cantalapiedra A, Caeiro J-R. Effects of glucosamine and risedronate alone or in combination in an experimental rabbit model of osteoarthritis. BMC Vet Res 2014;10(1):97.
Rouahi M, Gallet O, Champion E, Dentzer J, Hardouin P, Anselme K. Influence of hydroxyapatite microstructure on human bone cell response. J Biomed Material Res Part A 2006;78(2):222-35.
Takauti ACY, Futema F, de Brito Junior RB, Abrahão AC, Costa C, Queiroz C. Assessment of bone healing in rabbit calvaria grafted with three different biomaterials. Brazilian Dental Journal, 25(5): 379-384.
Toker H, Ozdemir H, Ozer H, Eren K. A comparative evaluation of the systemic and local alendronate treatment in synthetic bone graft: A histologic and histomorphometric study in a rat calvarial defect model. Oral surg Oral Med Oral Pathol Oral Radiol. 2012;114(5):S146-S52.
Uraz A, Gultekin SE, Senguven B, Karaduman B, Sofuoglu IP, Pehlivan S, et al. Histologic and histomorphometric assessment of eggshell-derived bone graft substitutes on bone healing in rats. J Clin Exper Dent 2013;5(1):e23.
Yadao RA, Lim GD, Pe LC, Valdez AM, Cristobal S, Sunico ATC, et al. Ostrich eggshell as an onlay bone-graft substitute for orbital blow-out fractures. Philippine J Ophthalmol 2004;29(3):127-30.
Yadegari M, Karimi M, Sheishi P, Derakhshan S. A radiographic survey of eggshell powder effect on tibial bone defect repair tested in dog. Human Vet Med 2015;7(4):392-6.
Yang C, Unursaikhan O, Lee JS, Jung UW, Kim CS, Choi SH. Osteoconductivity and biodegradation of synthetic bone substitutes with different tricalcium phosphate contents in rabbits. J Biomed Material Res Part B: App Biomaterial 2014;102(1):80-8.
Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain 1983;16(2):109-10.
Zingg M, Chowdhury K, Lädrach K, Vuillemin T, Sutter F, Raveh J. Treatment of 813 zygoma-lateral orbital complex fractures: New aspects. Arch Otolaryngol Head Neck Surg 1991;117(6):611-20.