Influence of dietary Moringa oleifera on broilers performance, intestinal microbial population and humoral immune competence

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DOI: https://doi.org/10.12681/jhvms.22224
Keywords:
M. oleifera chickens growth bacterial count HI
W.A. ABD EL-GHANY
Cairo University, Faculty of Veterinary Medicine, Department of Poultry Diseases, Giza, Egypt
R.D. ERAKY
Animal Health Research Institute (Damietta branch), 2Bacteriology Department, Damietta, Egypt
Abstract
This study was designed to evaluate the effect of using different levels of phytobiotic containing Moringa oleifera (M. oleifera) leaf powder (MOLP) on broiler chickens body growth performance parameters, intestinal microbial population, and the humoral immune response. Day-old Hubbard broiler chicks (n = 200) were randomly allocated into 4 treatment diet. Basal diet was supplied with treatments T0, T1, T2 and T3 representing (0, 1%, 5% and 7.5% MOLP); respectively. Chickens were kept under observation for 5 weeks. Body performance parameters, total viable bacterial and coliform counts and humoral immune response to Newcastle disease (ND) virus vaccine were detected using the haemagglutination inhibition (HI) test. The results revealed significant (P < 0.05) improvement in performance parameters in groups supplemented with dietary MOLP. However, the best significant (P < 0.05) performance was observed in the group fed 1% MOLP. There was no significant (P < 0.05) difference among treatments considering the total viable intestinal bacterial and coliform counts. Significant (P < 0.05) increase in the means of HI in dietary MOLP supplemented groups was observed, where the highest means were seen in 1% MOLP treated birds. In conclusion, dietary supplementation with MOLP could improve the performance parameters and the immune response while reducing the total viable intestinal bacterial and coliform counts of broiler chickens. The study recommended using of dietary level of 1% MOLP to improve performance, intestinal health, and immune competence.
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References
Aderinola OA, Rafiu TA, Akinwumi AO, Alabi TA, Adeagbo OA (2013) Utilization of Moringa oleifera leaf as feed supplement in broiler diet. Int J Food Agric Vet Sci 3: 94-102.
Agashe JL, Manwar SJ, Khose KK, Wade MR (2017) Effect of supplementation of Moringa oleifera leaf powder on growth performance of broilers. J Poult Sci Technol 5: 28-34.
Al-Husnan LA, Alkahtani MDF (2016) Impact of Moringa aqueous extract on pathogenic bacteria and fungi in vitro. Annals Agric Sci 61:247-250.
Al-Majali IS, Al-Oran SA, Hassuneh MR, Al-Qaralleh HN, Abu Rayyan W, Al-Thunibat OY, Mallah E, Abu-Rayyan A, Salem S (2017) Immunomodulatory effect of Moringa peregrina leaves, ex vivo and in vivo study. Cent Eur J Immunol 42: 231-238.
Association of Official Analytical Chemists (AOAC) 2000. Official Methods of Analysis. 17th edition. The Association, Washington DC. USA.
Banjo OS (2012) Growth and performance as affected by inclusion of moringa oleifera leaf meal in broiler chicks diet. J Biol Agric Healthcare 2: 35-39.
Bell DD, Weaver JrWD (2002) Commercial Chicken Meat and Egg Production. 5 Ed. Kluwer academic Publishers. USA.
Chollom SC, Agada GOA, Gotep JG, Mwankon SE, Dus PC, Bot YS, Nyango DY, Singnap CL, Fyaktu EJ, Okwori AEJ (2012) Investigation of aqueous extract of Moringa oleifera lam seed for antiviral activity against Newcastle disease virus in ovo. J Med Plant Res 6:3870-3875.
Divya ABM, Biswas AK, Yadav AS (2014) Effect of dietary Moringa oleifera leaves powder on growth performance, blood chemistry, meat quality and gut microflora of broiler chicks. Anim Nutr Feed Technol 14: 349-357.
Eze DC, Okwor EC, Ehirim CH, Ibu JO, Shoyinka SVO (2014) Comparative evaluation of Moringa oleifera and vacci-boost immunomodulators. S Asian JExploration Biol 4: 42-47.
Eze DC, Okwor EC, Okoye JOA, Onah DN (2013) Immunologic effects of Moringa oleifera methanolic leaf extract in chickens infected with Newcastle disease virus (kudu 113) strain. Afr J Pharmacol 7: 2231-2237.
Gomashe AV, Gulhane PA, Junghare MP, Dhakate NA (2014) Antimicrobial activity of Indian medicinal plants Moringa oleifera and Saraca Indica. Int J Curr Microbiol App Sci 3: 161-169.
Hashemipour H, Khaksar V, Rubio LA, Veldkamp T, van Krimpen MM (2016) Effect of feed supplementation with a thymol plus carvacrol mixture, in combination or not with an NSP degrading enzyme, on productive and physiological parameters of broilers fed on wheatbased diets. Anim Feed Sci Technol 211: 117-131.
Hong JC, Steiner T, Aufy A, Lien TF (2012) Effects of supplemental essential oil on growth performance, lipid metabolites and immunity, intestinal characteristics, microbiota and carcass traits in broilers. Livestock Sci 144: 253-262.
Hossam A, Abdelazem MA, Farag HS, Hamed A (2016) Some hemato-biochemical, bacteriological and pathological effects of Moringa oleifera leaf extract in broiler chickens. Int J Basic App Sci 5: 99-104.
ISO (1995). Recommendation of the meeting of the subcommittee, International Organization for Standardization, on meat and meat products. ISO/TC-36/Sc-6. The Netherlands. 10-18.
Khan I, Zaneb H, Masood S, Yousaf MS, Rehman HF, Rehman H (2017) Effect of Moringa oleifera leaf powder supplementation on growth performance and intestinal morphology in broiler chickens. J Anim Physiol Anim Nutr 101: 114-121.
Kout E, Moustafa ME, Riry FH, Mousa S, Hanan MAM, Alghonimy AH, Youssef SF (2015) Effect of using Moringa oleifera leaf meal on performance of Japanese quail. Egypt Poult Sci J 35: 1095-1108.
Kostadinović Lj, Popović S, Ljubojević Pelić D, Čabarkapa I, Đuragić O, Lević J (2019) Medicinal plants as natural alternative to coccidial synthetic drugs in broiler chicken production. J Agron Technol Eng Manag 2(5): 325-334.
Moez SI, Badawi AY, Omer HAA (2014) Assessment of antimicrobial effect of Moringa: In vitro and in vivo evaluation. Afr J Microbiol Res 8: 3630-3638.
Mousa MA, Osman AS, AbdelHady HAM (2017) Performance, immunology and biochemical parameters of Moringa oleifera and/or Cichorium intybus addition to broiler chicken ration. J Vet Med Anim Health 9: 255-263.
Moyo B, Oyedemi S, Masika PJ, Muchenje V (2012) Polyphenolic content and antioxidant properties of Moringa oleifera leaf extracts and enzymatic activity of liver from goats supplemented with Moringa oleifera leaves/sunflower seed cake. Meat Sci 91: 441-447.
Nkukwana TT (2012) Effect of Moringa oleifera leaf meal on growth performance, gut integrity, bone strength, quality and oxidative stability of meat from broiler chickens. Thesis Ph. D (Animal Sciences), Faculty of Science and Agriculture, University of Fort Hare, Alice, South Africa, 2–3.
National Research Council (NRC) (1994) Nutrient Requirements of Poultry. 9th Rev. ed., National Academy Press, Washington, DC.
Ochi EB, Elbushra ME, Fatur M, Abubakr Ismail O, Hafiz A (2015) Effect of moringa (Moringa oleifera lam) seeds on the performance and carcass characteristics of broiler chickens. J Natural Sci Res, 5: 66-73.
Okafor IN, Ezebuo FC, Azodo NT (2014) Effect of Moringa oleifera leaf protein concentrate supplemented feed on growth and nutritional parameters in broilers. World App Sci J 32: 133-138.
Olugbemi TS, Mutayoba SK, Lekule FP (2010) Effect of moringa (Moringa oleifera) inclusion in cassava based diets fed to broiler chickens. Int J Poult Sci 9: 363-367.
Onsare JG, Kaur H, Arora DS (2013) Antimicrobial activity of Moringa oleifera from different locations against some human pathogens. Acad J Med Plants 1: 080-091.
Onunkwo DN, George OS (2015) Effects of Moringa oleifera leaf meal on the growth performance and carcass characteristics of broiler birds. IOSR J Agric Vet Sci 8: 63-66.
Popović S, Kostadinović Lj, Đuragić O, Aćimović M, Čabarkapa I, Puvača N, Ljubojević Pelić D (2018) Influence of medicinal plants mixtures (Artemisia absinthium, Thymus vulgaris, Menthae piperitae and Thymus serpyllum) in broilers nutrition on biochemical blood status.J Agron Technol Eng Manag 1(1): 91-98.
SAS Institute (2000). SAS® User’s Guide: Statistics. Version & Edition. SAS Institute Inc., Cary, NC, USA.
Swayne DE, Glisson JR, Jackwood MW, Pearson JE, Reed WM (1998) A Laboratory Manual for the Isolation and Identification of Avian Pathogens. 4 Ed, American Association of Avian Pathologist Inc, Kennett Square, Pennsylvania, USA.
Tesfaye E, Animut G, Urge M, Dessie T (2013) Moringa olifera leaf meal as an alternative protein feed ingredient in broiler ration. Int J Poult Sci 12: 289-297.
Yang R, Chang L, Hsu J, Weng BBC, Palada MC, Chadha ML, Levawsseur V (2006) Nutritional and functional properties of Moringa leaves-from germplasm, to plant, to food, to health. Proceedings of the Workshop on Moringa and other Highly Nutritious Plant Resources: Strategies, Standards and Markets for a Better Impact on Nutrition in Africa, November, 16-18, 2006, Accra, Ghana, pp: 1-9.
Younis Mona EM, Elbestawy AR (2017) Water supplementation of Moringa oleifera and its effect on performance, blood antioxidant and immune response of two broiler breeds. J Biol Sci 17: 52-60.
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