Investigation of serum homocysteine levels in relation to age and sex

Sheep homocysteine gender differences age differences
Homocysteine is a non-proteinogenic and a derived amino acid in the methionine metabolism and is a risk factor for cardiovascular and many other metabolic diseases. In this study, the purpose was to determine serum homocysteine levels in healthy sheep based on differences in age and gender. 220 healthy Akkaraman sheep, composed of both females (n=55 lambs and 55 ewes) and males (n=55 lambs and 55 rams), were used as animal samples. The measurements of serum homocysteine concentrations were performed with ELISA-HCY kit. The levels of serum homocysteine of sheep were detected in ewes, female lambs, rams and male lambs as 2,91±0,50; 2,99±0,42; 11,22±3,10; 6,43±1,26 μmol/L, respectively. The primary intent of this study was to investigation and characterization the serum Hcy concentrations in healthy sheep broken down by different ages in both genders As a result, the serum homocysteine values that can constitute a reference value for healthy breeds of sheep were determined in this study.
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Carmel R, Jacobsen, D.W. (2001) Homocysteine in health and disease, Cambridge University Press, England.
Carson NAJ, Cusworth DC, Dentt CE, Field CMB, Neill DW, Westallt RG (1963) Homocystinuria: A New Inborn Error of Metabolism Associated with Mental Deficiency. Arch. Dis. Childl., 38, 425.
Coles, E.H. (1986) Veterinary Clinical Pathology 4th Edition. W.B. Saunders Co. Philadelphia.
Corrales F.J, Pérez-Mato I, Sánchez Del Pino M.M., Ruiz F., Castro C., Trevijano E.R., et al., (2002) Regulation of mammalian liver methionine adenosyltransferase. J Nutr 132:2377-2381.
Furlong JM, Sedcole JR, Sykes AR. (2010) An evaluation of plasma homocysteine in the assessment of vitamin B12 status of pasture-fed sheep. New Zealand Veterinary Journal. 58:11-16.
Finkelstein JD, Martin JJ. (2000) Homocysteine. The International Journal of Biochemistry and Cell Biology. 32:385-89.
Gibson JB, Carson NAJ, Neill DW (1964) Pathological findings in homocystinuria. Journal of Clinical Pathology. 17:427-437.
Graham IM., Daly LE, Refsum HM, Robinson K, Brattström LE, Ueland PM, Palma-Reis RJ., et all. (1997) Plasma homocysteine as a risk factor for vascular disease. The European Concerted Action Project. JAMA. 277:1775-81.
Hankey GJ, Eikelboom JW. (1999) Homocysteine and vascular disease. Lancet. 354:407-413.
Kozat S, Altuğ N, Yüksek N, Özkan C. (2011) Evaluation of the levels of homocysteine, troponin I, and nitric oxide in lambs with subclinical white muscle disease. Kafkas Univ Vet Fak Derg.17: 441-4.
Kumar A, Palfrey HA, Pathak R, Kadowitz PJ, Gettys TW, Murthy SN. (2017) The metabolism and significance of homocysteine in nutrition and healthy. Nutr Metab (Lond). 14: 78.
Martinez Y, Li X, Liu G, Bin P, Yan W, Más D, Valdivié M, Hu CA, Ren W, Yin Y. (2017) The role of methionine on metabolism, oxidative stress, and diseases. Amino Acids 49:2091-2098.
Masuda Y, Kubo A, Kokaze A, Yoshida M, Fukuhara N, Takashima Y. (2008) Factors associated with serum total homocysteine level in type 2 diabetes. Environ Health Prev Med. 13:148–155.
McCully, KS. (1969) Vascular pathology of homocysteinemia: implications for the pathogenesis of arteriosclerosis. Am J Pathol. 56:111-28.
Murín R, Vidomanová E, Kowtharapu BS, Hatok J, Dobrota D. (2017) Gen Physiol Biophys. Role of S-adenosylmethionine cycle in carcinogenesis. 36:513-520.
Mohammad FI, Awawdeh S, Saleh A, and Bashir NA. (2010) Total Level of Serum Homocysteine in Males and Females with Coronary Heart Disease of Different Age Groups American Journal of Biochemistry and Biotechnology. 6:116-119.
Piccione G, Casella S, Assenza A, Fazio F, Caola G. (2008) Evaluation of serum homocysteine and oxidative stress during lactation in ewes Czech J. Anim. Sci. 53: 462–465.
Ramakrishnan S, Sulochana KN, Lakshmi S, Selvi R, Angayarkanni N. (2006) Biochemistry of homocysteine in health and diseases Indian J Biochem Biophys. 43:275-283.
Ravaglia G, Forti P, Maioli F, Martelli M, Servadei L, Brunetti N, Porcellini E, Licastra F. (2005) Homocysteine and folate as risk factors for dementia and Alzheimer disease. Am J Clin Nutr. 82:636-643.
Razavi SM, Nazifi S, Mokhtari N, and Rakhshandehroo E. (2015) Correlations Among the Level of Homocysteine, Antioxidant Enzymes, Antioxidant Vitamins and Lipid Peroxidation of Erythrocytes in Malignant Ovine Theileriosis. Research Journal of Parasitology. 10:42-49.
Refsum H. (1998) Homocysteine and cardiovascular disease. Ann Rev Med. 49, 31–62.
Rezaei SA, Dalir-Naghadeh B. (2009) Association of plasma and heart homocysteine and blood malondialdehyde with cardiovascular diseases induced by acute selenium deficiency in lambs. Small Rum Res. 83:22-28.
Richards JB. (2008) Homocysteine levels and leukocyte telomere length. Atherosclerosis. 200:271-277.
Robinson K, Mayer E, Jacobsen DW. (1994) Homocysteine and coronary artery disease. Cleveland Clinic Journal of Medicine. 61:438-450.
Škovierová H, Vidomanová E, Mahmood S, Sopková J, Drgová A, Červeňová T, Halašová E, Lehotský J. (2016) The Molecular and Cellular Effect of Homocysteine Metabolism Imbalance on Human Health. Int J Mol Sci.17:1733.
Varga EA. (2005) Cardiology patient pages. Homocysteine and MTHFR mutations: relation to thrombosis and coronary artery disease. Circulation. 111:289-293.
Wijekoon EP, Brosnan ME, Brosnan JT. Chapter Ed: Dhalla N.S., (2006) Chapter 15: Homocysteine Metabolism, Vol.1: Biochemistry of Atherosclerosis. Advances in Biochemistry in Health and Disease, p:329-357. (ISBN-10: 0-387-31252-8 / ISBN-13: 978-0387-31252-1) Springer Science, Business Media, LLC.
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