Molecular profile of avian pathogenic Escherichia coli (APEC) from poultry associated with colibacillosis in Algeria

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DOI: https://doi.org/10.12681/jhvms.23666
Keywords:
E. coli APEC virulence factors avian colibacillosis Algeria
M. LOUNIS
Département des Sciences Agrovétérinaires, Faculté des Sciences de la Nature et de la Vie, Université Ziane Achour, Djelfa 17000, Algérie.
G. ZHAO
Laboratory of Quality and Safety Risk Assessment for Animal Products, China Animal Health and Epidemiology Center, Qingdao, China.
Y. LI
Laboratory of Quality and Safety Risk Assessment for Animal Products, China Animal Health and Epidemiology Center, Qingdao, China.
Y. GAO
Laboratory of Quality and Safety Risk Assessment for Animal Products, China Animal Health and Epidemiology Center, Qingdao, China.
J. WANG
Laboratory of Quality and Safety Risk Assessment for Animal Products, China Animal Health and Epidemiology Center, Qingdao, China.
M. OUMOUNA
Faculté des Sciences, Université Yahia Fares, Médéa, Algérie.
K. OUMOUNA
Faculté des Sciences, Université Yahia Fares, Médéa, Algérie.
Abstract

The objective of the present study was the detection of virulence-associated genes of E. coli isolated from chicken with colibacillosis. Seventeen (17) APEC isolates were examined by two panels of PCRs for the presence of 11 genes described for avian pathogenic (hlyF, iroN, iss, ompT, iutA and fimC) and diarrheagenic (eae, stx, est, elt, ipaH and aggR) E. coli. Results revealed that none of the APEC isolates harbored the genes eae, stx, est, elt, ipaH and aggR. In another hand, 88.2% of the isolates were positive for 3 or more of the virulence genes hlyF, iroN, iss, ompT, iutA and fimC. Also, 35.3% of the isolates harbored all the six genes. Genes fimC (88.2%), iss (82.3%) and ompT (76.5%) were the most prevalent while genes hylF, iutA and iroN which were present with the same frequency (52.9%) were mostly associated with highly pathogenic strains.

 

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