Heat killed Dietzia maris reduces lipopolysaccharide-induced inflammatory responses in murine adherent peritoneal cells

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DOI: https://doi.org/10.12681/jhvms.24165
Keywords:
Adherent peritoneal cells Anti-inflammation Dietzia maris IL-6 Lipopolysaccharide
K. NOFOUZI
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
B. GHAHVECHI-DIZAJI
Department of Animal Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran
G.H. ZARRINI
Department of Animal Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran
B. BARADARAN
Immunology Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
G.H. HAMIDIAN
Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
R. SAFARALIZADEH
Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
G. MCINTYRE
Centre for Infectious Diseases and International Health, Windeyer Institute of Medical Sciences, University College London, 46 Cleveland Street, London, United Kingdom
P. RIPLEY
BioEos Ltd., Mill House, Claygate, Marden, Near Tonbridge, Kent, UK
S. MOUSAVI
Department of Food Hygiene and Aquatic Animals, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Abstract

Dietzia maris (D. maris) is a gram-positive, aerobic, mycolic acid-containing actinomycete without mycelium. Actinomycetes such as Tsukamurella inchonensis reduce lipopolysaccharide- induced inflammatory responses in activated murine peritoneal macrophages. Here, the effects of D. maris on LPS-induced inflammatory responses were examined in mouse adherent peritoneal cells. D. maris was grown, harvested, and washed. Suspensions were standardized by wet weight, re-suspended in borate-buffered saline, and autoclaved. For in vivo study, each mouse was orally administered by bacterial suspension (5 × 107, 1 × 108 and 2 × 108 CFU/Mouse) consecutively for seven days. Control animals received the same amount of phosphate-buffered saline (PBS). Adherent peritoneal cells were harvested for in vitro experiments. Cells were lavaged and plated in RPMI 1640 medium, stimulated with LPS (100 ng/ml), and incubated for 2 h. Afterward, non-adherent cells were removed followed by adding freshly prepared medium. Supernatants (50 μl) were collected, centrifuged, mixed with Griess reagent, and the absorbance was measured at 560 nm. D .maris inhibited LPS-stimulated nitric oxide (NO) production in murine macrophages at concentrations of 5 × 107, 1 × 108 and 2 × 108 CFU/Mouse. Also, D. maris decreased LPS-induced production of pro-inflammatory cytokines of interleukin (IL)-6 at all doses. By contrast, tumor necrosis factor (TNF)-α was not effected by D. maris treatment of mice. Our results indicate that D. maris is a potent inhibitor of LPS-induced NO production. D. maris may be useful as a novel agent for the chemoprevention of inflammatory disease.

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