Age-and sex-related changes in selected hematological parameters, lipid peroxidation and erythrocytes osmotic fragility of Turkish Angora cats

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Published: Apr 29, 2022
DOI: https://doi.org/10.12681/jhvms.25515
Keywords:
Erythrocyte values MDA membrane stability aging oxidative stress
Ruhi Kabakci
Department of Physiology, Faculty of Veterinary Medicine, Kırıkkale University
https://orcid.org/0000-0001-9131-0933
Abstract
This study was conducted to investigate the changes in selected hematological parameters, lipid peroxidation and osmotic fragility of erythrocytes in Angora cats depending on the age and gender. For this purpose, the blood samples were collected from 9 young and 14 adult cats which also classified as male (n=12) and female (n=11). Following hematological analysis, samples were washed with PBS by centrifugation and 10% hematocrit suspension was prepared from the erythrocytes pellet for osmotic fragility test. The concentration of malondialdehyde (MDA) was also measured from lysed erythrocyte to determine lipid peroxidation level. Red blood cells (RBCs), hemoglobin, and hematocrit were high in adults while mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration were high in young cats. Erythrocyte MDA level was also higher in adult cats than in young cats. There was no significance in these parameters between male and female cats. Findings of fragility tests were showed that erythrocytes of young and male cats were more susceptible to hypotonic NaCl solutions than those of adult and female cats, respectively. It was concluded that erythrocytes related parameters in Angora cats changed depending on the age rather than gender except stability of RBCs.
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References
Akila VP, Harishchandra H, D'souza V, and D'souza B (2007) Age related changes in lipid peroxidation and antioxidants in elderly people. Indian J Clin Biochem 22: 131-134.
Andriichuk A, Tkachenko H, and Kurhaluk N (2014) Gender differences of oxidative stress biomarkers and erythrocyte damage in well-trained horses during exercise. J Equine Vet Sci 34: 978-985.
Asri RS, Ramin A, and Koukalanifar M (2006) Evaluation of correlation between serum vitamin e status and the erythrocyte osmotic fragility in sheep. J Vet Sci 61: 155-159.
Aydilek N, and Şimşek H (2006) Yaşlanmanın kısraklarda plazma oksidan ve antioksidan parametreler üzerine etkisi. J Fac Vet Med Univ Erciyes 3: 73-77.
Azeez O, Olayemi F, and Olanrewaju J (2011) Age and sex influences on the haematology and erythrocyte osmotic fragility of the nigerian turkey. Res J Vet Sci 4: 43-49.
Basarab J, Berg R, and Thompson J (1980) Erythrocyte fragility in" double-muscled" cattle. Can J Anim Sci 60: 869-876.
Beutler E (1990) Osmotic fragility. in Williams JW, Beutler E and Erslev AJ (eds.) Hematology, 4th ed, McGraw Hill Publishing Co, New York: pp 1726–1728
Buege JA, and Aust SD (1978) Microsomal lipid peroxidation. in Methods enzymol, 1st ed, Elsevier: pp 302-310
Drabkin DL, and Austin JH (1932) Spectrophotometric studies i. Spectrophotometric constants for common hemoglobin derivatives in human, dog, and rabbit blood. J Biol Chem 98: 719-733.
Durotoye L (1987) Effect of sex, pregnancy and lactation on the osmotic fragility of the west african dwarf sheep. Bull Anim Health Prod Afr 35: 29-33.
Durotoye L, and Oyewale J (1988) A comparative study of erythrocyte osmotic fragility between the guinea fowl (numidea meleagris galeata) and the nigerian domestic fowl (gallus domesticus). Bull Anim Health Prod Afr 36: 73-76.
Elitok B (2012) Reference values for hematological and biochemical parameters in saanen goats breeding in afyonkarahisar province. Kocatepe Vet J 5: 2012517117-2012517121.
Gaál T, Speake B, Mezes M, Noble R, Surai P, and Vajdovich P (1996) Antioxidant parameters and ageing in some animal species. Comp Haematol Int 6: 208-213.
Grinna LS (1977) Changes in cell membranes during aging. Gerontology 23: 452-464.
Habibu B, Kawu M, Makun H, Aluwong T, Yaqub L, Ahmad M, Tauheed M, and Buhari H (2014) Influence of sex, reproductive status and foetal number on erythrocyte osmotic fragility, haematological and physiologic parameters in goats during the hot-dry season. Vet Med (Praha) 59: 479-490.
Halliwell B (1994) Free radicals, antioxidants, and human disease: Curiosity, cause, or consequence? Lancet (British edition) 344: 721-724.
Igbokwe N (2018) A review of the factors that influence erythrocyte osmotic fragility. Sokoto J Vet Sci 16: 1-23.
Kohn B, Goldschmidt MH, Hohenhaus AE, and Giger U (2000) Anemia, splenomegaly, and increased osmotic fragility of erythrocytes in abyssinian and somali cats. J Am Vet Med Assoc 217: 1483-1491.
Matsubara LS, and Machado P (1991) Age-related changes of glutathione content, glutathione reductase and glutathione peroxidase activity of human erythrocytes. Braz J Med Biol Res 24: 449.
Mosior M, and Gomułkiewicz J (1988) Osmotic properties of bovine erythrocytes aged in vivo. Gen Physiol Biophys 7: 73-79.
Ogunyemi AD, and Olayemi FO (2016) Comparative assessment of erythrocyte osmotic fragility in two breeds of dog: Nigerian indigenous breed and the german shepherd. Comp Clin Path 25: 79-83.
Ojo O, Kabutu F, Bello M, and Babayo U (2006) Inhibition of paracetamol-induced oxidative stress in rats by extracts of lemongrass (cymbropogon citratus) and green tea (camellia sinensis) in rats. Afr J Biotechnol 5: 1227-1232.
Olayemi F (2007) The effect of sex on the erythrocyte osmotic fragility of the nigerian white fulani and ndama breeds of cattle. Top Vet 25: 106-111.
Olayemi F, Azeez I, Ogunyemi A, and Ighagbon F (2009) Study on erythrocyte values of the nigerian indigenous dog. Folia Vet 53: 65-67.
Olorunshola K, Eze K, and Achie L (2012) Effect of road transport stress on erthrocyte osmotic fragility (eof) of healthy young adult nigerians during the harmattan season. Niger J Physiol Sci 27: 157-164.
Özkan C, Kozat S, Kaya A, and Akgül Y (2016) Some hematological and biochemical parameter levels in healthy van cats at different age and gender. Eurasian J. Vet. Sci 32: 214-219.
Perk K, Hort I, and Perri H (1964) The degrees of swelling and osmotic resistance in hypotonic solutions of erythrocyte from various domestic animals. Refuah vet 20: 122-128.
Rifkind JM, Araki K, and Hadley EC (1983) The relationship between the osmotic fragility of human erythrocytes and cell age. Arch Biochem Biophys 222: 582-589.
Sako T, Takeda S, Shibuya M, Koyama H, Uchino T, and Motoyoshi S (1989) Seasonal changes in the phospholipids and fatty acid composition of the bovine erythrocyte membrane. The Japanese journal of veterinary science 51: 1243-1245.
Salar-Amoli J, and Baghbanzadeh A (2010) Oxidative stress in shaal sheep in different age groups. Turk J Vet Anim Sci 34: 379-383.
Simsek O, Cinar M, and Arikan S (2015) Changes in selected hematology and serum biochemistry in turkish angora cats (felis catus) during growth period. J adv vet 2: 34-39.
Simsek O, Ekici H, Cinar M, Atmaca N, Arikan S, and Guner B (2015) Heavy metal accumulatıon and oxıdatıve-antıoxıdatıve status in angora goats dependıng on age. Fresenius Environ Bull 24: 2659-2663.
Slappendel R (1998) Abnormal osmotic fragility of erythrocytes in dogs and cats. Vet Q 20: S38-S39.
Sönmez N, and Ağaoğlu ZT (2010) Van kedilerinde kardiak troponin seviyelerinin araştırılması. Van Vet J 21: 21-25.
Spada E, Antognoni MT, Proverbio D, Ferro E, Mangili V, and Miglio A (2015) Haematological and biochemical reference intervals in adult maine coon cat blood donors. J Feline Med Surg 17: 1020-1027.
Şimşek Ö, Arıkan Ş, and Çınar M (2015) Reference values for selected hematological and biochemical blood parameters from prepregnancy to advanced gestation in angora cats. Turk J Vet Anim Sci 39: 29-33.
Theml H, Diem H, and Haferlach T (2004) Color atlas of hematology: Practical microskopic and clinical diagnosis. Thieme: New York.
Tritschler C, Mizukami K, Raj K, and Giger U (2016) Increased erythrocytic osmotic fragility in anemic domestic shorthair and purebred cats. J Feline Med Surg 18: 462-470.
Turgut K (2000) Veteriner klinik laboratuvar teshis. Bacıvanlar Basım Sanayi AŞ: Konya.
Tyan ML (1982) Age-related increase in erythrocyte oxidant sensitivity. Mech Ageing Dev 20: 25-32.
van Ginkel G, and Sevanian A (1994) Lipid peroxidation-induced membrane structural alterations. Methods Enzymol 233: 273-288.
Yiğit AA, and Kabakçı R (2018) Çevre kirleticilerden ağır metallerin hayvanlarda hematopoetik sistem üzerine etkileri. Turkiye Klinikleri J Vet Sci Pharmacol Toxicol-Special Topics 4: 9-15.
Yiğit AA, Kısa Ü, Arıkan Ş, Akçapınar H, and Taşdemir U (2002) Sakız x karayaka melezi gı koyunlarının kan parametreleri üzerine cinsiyet ve yaşın etkisi. Ankara Univ Hekim Fak Derg 49: 101-106.