Investigation of Seroprevalence of Toxoplasmosis in Horses and Donkeys in Muş Province of Turkey

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Published: Apr 29, 2022
DOI: https://doi.org/10.12681/jhvms.25571
Keywords:
Donkey Horse SFDT Toxoplasma gondii Toxoplasmosis
Akin Kirbas
Yozgat Bozok University, Faculty of Veterinary Medicine, Department of Internal Medicine
https://orcid.org/0000-0001-9159-3240
Abstract

The purpose of this study was to determine the seroprevalence of Toxoplasmosis in equidae in the province of Muş, Turkey. The study material consisted of 210 equidae including 159 horses and 51 donkeys in Mus province. In serum samples, anti-Toxoplasma gondii antibodies and titers were detected using Sabin Feldman Dye Test (SFDT). Seropositivity was found in 115 (54.76 %) of the 210 equidae tested in the study. The rate of seropositivity in donkeys (92.16%) was higher than the rate in horses (42.77%), and statistical significance was observed (P<0.001). T. gondii antibody was detected in 68 (42.77%) of the horse sera examined by SFDT. When T. gondii seropositivity was evaluated according to gender, it was found to be 47.92% in females and 32.92% in males. No statistical difference was observed between the gender groups (P> 0.05). When T. gondii seropositivity was evaluated according to age, seropositivity rate in the equidae older than 10 years was found to be higher as 46.67%, but no statistical significance was observed among the age groups. T. gondii antibody was detected in 47 (92.16%) of donkey sera examined by SFDT. When T. gondii seropositivity was evaluated according to gender, the rate of seropositivity was found to be 89.47% in females and 93.75% in males. No statistical significance was observed between the gender groups (P>0.05). When T. gondii seropositivity was evaluated according to age, the seropositivity rate in those older than 10 years was found to be higher as 96.30%, but  no statistical significance was observed among the age groups (P>0.05). As a result of scanning the equidea in Muş province by SFDT, seropositivity rate was found as 42.77% in horses and 92.16% in donkeys.

Keywords: Donkey, Horse, SFDT, Toxoplasma gondii, Toxoplasmosis

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References
Akca A, Babur C, Arslan MO, Gicik Y, Kara M, Kilic S (2004) Prevalence of antibodies to Toxoplasma gondii in horses in the province of Kars, Turkey. Vet Med-Czech 49: 9-13.
Akkan HA, Tütüncü M, Karaca M, Çiftçi İH, Yüksek N, Ağaoğlu ZT (2001) Seroprevalence of Toxoplasma gondii in horses in Van province. Van Vet J 12: 43-44.
Aktas M, Babür C, Köroğlu E, Dumanlı N (1999) Detection of Anti-Toxoplasma gondii Antibodies Using Sabin-Feldman Dye Test in Horses in Sultansuyu Agriculture Unit in Malatya. Fırat Univ Sag Bil Derg 13: 89-91.
Almeida JC, Vidotto O, Ferreira EP, Ribeiro LP, Mongruel AC, Vieira TS, Freire RL, Mota RA, Vieira RF (2017) Serosurvey of anti-Toxoplasma gondii antibodies in sport horses from Paraiba state, Northeastern Brazil. Acta Parasitol 62: 225-227.
Aslantaş Ö, Babür C, Kılıç S (2001) Seroprevalence of Brucella and Toxoplasmosis in Horses in Kars Region. Etlik Vet Mikrobiyol Derg 12: 1-7.
Babur C, Çakmak A, Biyikoğlu G, Pişkin CF (1998) Ankara Atatürk orman çiftliği hayvanat bahçesi vahşi hayvanlarını beslemek için kesilen atlarda Anti-Toxoplasma gondii antikorlarının Sabin Feldman boya testi ile saptanması. Turkiye Parazitol Derg 22: 174-176.
Babur C, Yağci Ş, Sert H, Yaman N, Ateş C, Karaer Z. Serodiagnose of Toxoplasmosis ın Horses of Health Refik Saydam Hıfzısıhha Centre Serum Ranch. Etlik Vet Mikrobiyol Derg 1997, 9: 1-5.
Balkaya I, Babur C, Celebi B, Utuk AE (2011) Seroprevalence of Toxoplasmosis in donkeys in Eastern Turkey. Isr J Vet Med 66: 39-42.
Boughattas S, Bergaoui R, Essid R, Aoun K, Bouratbine A (2011) Seroprevalence of Toxoplasma gondii İnfection Among Horses in Tunisia. Parasites Vectors. 4: 218.
Dubey JP (1998) Re-examination of resistance of Toxoplasma gondii tachyzoites and bradyzoites to pepsin and trypsin digestion. Parasitology. 116(Pt1): 43-50.
Dubey JP, Beattie CP: Toxoplasmosis of animals and man. 1th Edition. Boca Raton, FL 33431, CRC Press, Inc., 1998, 220.
Dubey JP, Ness SL, Kwok OCH, Choudhary S, Mittel LD, Divers TJ (2014) Seropositivity of Toxoplasma gondii in domestic donkeys (Equus asinus) and isolation of T. gondii from farm cats. Vet Parasitol 199: 18-23.
El-Ghaysh A (1998) Seroprevalence of Toxoplasma gondii in Egyptian donkeys using ELISA. Vet Parasitol 80: 71-73.
Garcia-Bocanegra I, Cabezon O, Arenas-Montes A, Carbonero A, Dubey JP, Perea A, Almeria S (2012) Seroprevalence of Toxoplasma gondii in equids from Southern Spain.Int Parasitol 61: 421-424.
Gazyaǧci S, Macun HC, Babür C (2011) Investigation of seroprevalance of toxoplasmosis in mares and stallions in Ankara province, Turkey. Iran J Vet Res 12: 354-356.
Göz Y, Babür C, Aydin A, Kiliç S (2007) Seroprevalence of toxoplasmosis, brucellosis and listeriosis in horses in Hakkari, eastern region of Turkey. Rev Med Vet 158: 534-539.
Güçlü Z, Karaer Z, Babür C, Kılıç S (2007) Investigation of Toxoplasma gondii antibodies in sport horses bred in Ankara province. Turkiye Parazitol Derg 31: 264-267.
Haridy FM, Saleh NM, Khalil HH, Morsy TA (2010) Anti-Toxoplasma gondii antibodies in working donkeys and donkey's milk in greater Cairo, Egypt. J Egypt Soc Parasitol 40: 459-464.
Haridy FM, Shoukry NM, Hassan AA Morsy TA (2009) ELISA-seroprevalence of Toxoplasma gondii in draught horses in Greater Cairo, Egypt. J Egypt Soc Parasitol 39: 821-826.
Inci A, Babur C, Aydin N, Cam Y (2002) The Investigation on the Seroprevalence of Toxoplasma gondii (Nicolle ve Manceaux, 1908) and Listeria monocytogenes in Equids Around Kayseri. Fırat Univ Sag Bil Derg 16: 181-185.
Jewell ML, Frenkel JK, Johnson KM, Reed V, Ruiz A (1972) Development of Toxoplasma oocysts in neotropical felidae. Am J Trop Med Hyg 21: 512-517.
Kar S, Güven E: Sinir Sisteminde ve Kaslarda Görülen Protozoon Hastalıkları: Toxoplasmosis. In: Özcel MA, Köroğlu E, Kar S. (Eds): Veteriner Hekimliğinde Parazit Hastalıkları. Turkiye Parazitol Derg, 2016, pp. 375-378.
Karatepe B, Babür C, Karatepe M, Kılıç S (2010) Seroprevalence of toxoplasmosis in horses in Niğde Province of Turkey. Trop Anim Health Prod 42: 385-389.
Kim K, Weiss LM (2008) Toxoplasma: the next 100 years. Microb. Infect. 10: 978-984.
Klun I, Uzelac A, Villena I, Mercier A, Bobić B, Nikolić A, Rajnpreht I, Opsteegh M, Aubert D, Blaga R, van der Giessen J, Djurković-Djaković O, (2017) The first isolation and molecular characterization of Toxoplasma gondii from horses in Serbia. Parasites &Vectors 10: 167.
Kniel KE, Lindsay DS, Sumner SS, Hackney CR, Pierson MD, Dubey JP (2002) Examination of attachment and survival of Toxoplasma gondii oocysts on raspberries and blueberries. J Parasitol Res 88: 790-793.
Liu Q, Wang ZD, Huang SY, Zhu XQ (2015) Diagnosis of toxoplasmosis and typing of Toxoplasma gondii. Parasites & Vectors 8: 292.
Machacova T, Bartova E, Di Loria A, Sedlak K, Mariani U, Fusco G, Fulgione D, Veneziano V, Dubey JP (2014) Seroprevalence of Toxoplasma gondii in donkeys (Equus asinus) in Italy. J Vet Med Sci 76: 265-267.
Magalhães FJR, Ribeiro-Andrade M, Souza FM, Lima Filho CDF, Biondo AW, Vidotto O, Navarro IT, Mota RA (2017) Seroprevalence and spatial distribution of Toxoplasma gondii infection in cats, dogs, pigs and equines of the Fernando de Noronha Island. Parasitol Int 66: 43-46.
Miao Q, Wang X, She LN, Fan YT, Yuan FZ, Yang JF, Zhu XQ, Zou FC (2013) Seroprevalence of Toxoplasma gondii in horses and donkeys in Yunnan Province, Southwestern China. Parasites & Vectors 6: 168.
Miller NL, Frenkel JK, Dubey JP (1972) Oral Infections with Toxoplasma Cysts and Oocysts in Felines, Other Mammals, and in Birds. J Parasitol 58: 928-937.
Mohamed-Cherif A, Ait-Oudhia K, Khelef D (2015) Detection of anti-Toxoplasma gondii antibodies among horses (Equus caballus) and donkeys (Equus asinus) in Tiaret province, northwestern Algeria. Rev Med Vet 166: 271-274.
Ozkan AT, Babur C, Dundar B, Piskin FC (2002) Investigation of anti-Toxoplasma gondii antibodies using the Sabin-Feldman test (SFT) in the horses in some cities of Southeast Anatolia Region. Etlik Vet Mikrobiyol Derg 13: 16-18.
Paştiu AI, Györke A, Kalmár Z, Bolfă P, Rosenthal BM, Oltean M, Villena I, Spînu M, Cozma V (2015) Toxoplasma gondii in horse meat intended for human consumption in Romania. Vet Parasitol 212: 393-395.
Pomares C, Ajzenberg D, Bornard L, Bernardin G, Hasseine L, Dardé ML, Marty P (2011) Toxoplasmosis and horse meat, France. Emerg. Infect. Dis. 17: 1327-1328.
Razmi GR, Abedi V, Yaghfoori S (2016) Serological study of Toxoplasma gondii infection in Turkoman horses in the North Khorasan Province, Iran. J Parasit Dis 40: 515-519.
Ribeiro MJ, Rosa MH, Bruhn FR, Garcia AM, Rocha CM, Guimarães AM (2016)
Seroepidemiology of Sarcocystis neurona, Toxoplasma gondii and Neospora spp. among horses in the south of the state of Minas Gerais, Brazil. Braz J Vet Parasitol 25: 142-150.
Sabin AB, Feldman HA (1948) Dyes as microchemical indicators of a new immunity phenomen affecting a proton parasite (Toxoplasma). Sci 108: 660-663.
Villa L, Gazzonis AL, Álvarez-García G, Diezma-Díaz C, Zanzani SA, Manfredi MT (2018) First detection of anti-Besnoitia spp. specific antibodies in horses and donkeys in Italy. Parasitol Int 67: 640-643.
Zhang XX, Shi W, Zhang NZ, Shi K, Li JM, Xu P, Zhao Q, Du R (2017) Prevalence and genetic characterization of Toxoplasma gondii in donkeys in northeastern China. Infect Genet Evol 54: 455-457.
Zhou M, Cao S, Sevinc F, Sevinc M, Ceylan O, Liu M, Wang G, Moumouni PF, Jirapattharasate C, Suzuki H, Nishikawa Y, Xuan X (2016) Enzyme-linked immunosorbent assays using recombinant TgSAG2 and NcSAG1 to detect Toxoplasma gondii and neospora caninum-specific antibodies in domestic animals in Turkey. J Vet Med Sci 78: 1877-1881.