The effect of the challenge with Newcastle disease virus on the gastrointestinal bacterial population in Japanese quail (Coturnix japonica)

Published: Jul 27, 2022
Updated: 2022-07-27
2022-07-27 (2)
birds Escherichia coli gut lactobacillus verogenic NDV
F Talazadeh
RA Jafari
O Behrouzinasab
E Rahimi Sardo

Newcastle disease (ND), caused by virulent strains of Newcastle disease virus (NDV), is a devastating disease of poultry worldwide. The effect of a challenge with Newcastle disease virus on bacterial population in quail is poorly documented, so for this purpose, a total of 100 day-old Japanese quail were purchased and divided into 2 equal groups randomly. Each group was divided into 2 subgroups of 25 quails. The birds in group A challenged with a velogenic chicken isolate of Newcastle disease virus. The birds in group B did not challenge with Newcastle disease virus as the control group. For the determination of lactobacillus counts in the intestine and crop of Japanese quail, at the end of the period, 10 birds of each subgroup (20 birds of each treatment) were chosen randomly. One gram of the crop and ileocecal content were taken and cultured on MRS for determination of lactobacillus counts. The colony-forming units of Escherichia coli in digesta of ileocecal on Mac Conkey agar were investigated. Also, crop and ileocecal content of dead birds were taken and cultured on MRS and Mac Conkey agar. The results of this study showed that challenge with a velogenic chicken isolate of Newcastle disease virus could increase colony-forming units of Escherichia coli in group A compared to the control group. Also, it reduced lactobacillus counts of intestine and crop compared to the control group. So it concluded that velogenic chicken isolate of Newcastle disease virus influences microflora of intestine and crop of Japanese quail. 

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Roto SM, Kwon YM, Ricke SC. Applications of In Ovo Technique for the Optimal Development of the Gastrointestinal Tract and the Potential Influence on the Establishment of Its Microbiome in Poultry. Frontiers in Veterinary Science 2016; 3 (63) . doi: 10.3389/fvets.2016.00063.
Varmuzova K, Kubasova T, Davidova-Gerzova L, Sisak F, Havlickova H, Sebkova A, et al. Composition of gut microbiota influences resistance of newly hatched chickens to Salmonella enteritidis infection. Frontiers in Microbiology 2016; 7:957. doi: 10.3389/fmicb.2016.00957.
Macpherson AJ , Harris NL. Interactions between commensal intestinal bacteria and the immune system. Nature Reviews Immunology 2004, 4: 478–485.
Ouwehand A, Isolauri E, Salminen S. The role of the intestinal microflora for the development of the immune system in early childhood. European Journal of Nutrition 2002, 41: i32–i37.
Scholz-Ahrens KE, Ade P, Marten B,Weber P, TimmW, A6il Y, Glu¨er CC, et al. Prebiotics, probiotics, and synbiotics affect mineral absorption, bone mineral content, and bone structure. Journal of Nutrition 2007, 137: 838S–846S.
Hamilton-Miller JM. The role of probiotics in the treatment and prevention of Helicobacter pylori infection. International Journal of Antimicrobial Agents 2003, 22: 360–366.
Guarner F, Malagelada JR. Gut flora in health and disease. Lancet 2003, 361: 512–519.
Lalles JP, Bosi P, Smidt H, Stokes CR. Nutritional management of gut health in pigs around weaning. Proceedings of the Nutrition Society 2007, 66: 260–268.
Huang C, Qiao S, Li D, Piao X, Ren J. Effects of lactobacilli on the performance, diarrhea incidence, VFA concentration and gastrointestinal microbial flora of weaning pigs. Asian-Australasian Journal of Animal Sciences 2004, 17: 401–409.
Liu JK, Hou XL, Wei CH, Yu LY, He XJ, Wang GH, Lee JS, et al. Induction of immune responses in mice after oral immunization with recombinant Lactobacillus casei strains expressing enterotoxigenic Escherichia coli F41 fimbrial protein. Applied and Environmental Microbiology 2009, 75: 4491–4497.
Heravi RM, Kermanshahi H, Varasteh A, Sankian M, Mousavi AH. Identification and isolation of Lactobacilli in gastrointestinal tract of broiler chickens using 16S ribosomal DNA sequencing method. World Academy of Science and Technology Conference 2009, 09-22.
Amit-Romach E, Sklan D, Uni Z. Microflora ecology of the chicken intestine using 16S ribosomal DNA primers. Poultry Science 2004, 83: 1093–1098.
Dumonceaux TJ, Hill JE, Hemmingsen SM, Van Kessel AG. Characterization of intestinal microbiota and response to dietary virginiamycin supplementation in the broiler chicken. Applied and Environmental Microbiology 2006, 72: 2815–2823.
Ehrmann MA, Kurzak P, Bauer J, Vogel RF. Characterization of lactobacilli towards their use as probiotic adjuncts in poultry. Journal of Applied Microbiology 2002, 92: 966–975.
Botes M, Loos B, van Reenen CA, Dicks LM. Adhesion of the probiotic strains Enterococcus mundtii ST4SA and Lactobacillus plantarum 423 to Caco-2 cells under conditions simulating the intestinal tract, and in the presence of antibiotics and anti-inflammatory medicaments. Archives of Microbiology 2008, 190: 573–584.
Bateup JM, McConnell MA, Jenkinson HF, Tannock GW. Comparison of Lactobacillus strains with respect to bile salt hydrolase activity, colonization of the gastrointestinal tract, and growth rate of the murine host. Applied and Environmental Microbiology 1995, 61: 1147–1149.
Talazadeh F, Mayahi M, Zeinali S. The effect of Aquablend Avian probiotic ® includingLactobacillus,StreptococcusandBifidobacteriumon systemic antibody responseagainst Newcastle and Influenza disease vaccine in broiler chickens. International Journal of Enteric Pathogens 2016, 4(2), doi: 10.17795/ijep35689
Vaughan EE, Schu F, Heilig H, Zoetendal EG, de Vos WM, Akkermans AD. A molecular view of the intestinal ecosystem. Current issues in intestinal microbiology 2000, 1: 1–12.
Ma XX, Wang Q, Li HM, Xu CT, Cui N, Zhao XM. 16S rRNA genes Illumina sequencing revealed differential cecal microbiome in specific pathogen free chickens infected with different subgroup of avian leukosis viruses. Veterinary Microbiology 2017, 195–204. doi: 10.1016/j.vetmic.2017.05.016.
Stanley D, Wu SB, Rodgers N, Swick RA, Moore RJ. Differential responses of cecal microbiota to fishmeal, Eimeria and Clostridium perfringens in a necrotic enteritis challenge model in chickens. PLoS One 2014, 9:e104739. doi: 10.1371/journal.pone.0104739.
Alexander DJ. Newcastle disease in ostriches (Struthio camelus) - a review. Avian Pathology 2000, 29: 95-100.
Talazadeh F, Mayahi M, nofouzi k, Golzari E, Chegini R. The Effect of Tsukamurella inchonensis Bacterin on the Immune Response Against Influenza and Newcastle Disease Vaccines in Broiler Chickens. International Journal of Enteric Pathogens 2016;4(4): e37107. doi: 10.15171/ijep.2016.17
Jorgensen PH, Lomniczi B, Manvell RJ, Holm E, Alexander DJ. Isolation of avian paramyxovirus type 1 (Newcastle disease) viruses from a flock of ostriches (Struthio camelus) and emus (Dromaius novaehollandiae) in Europe with inconsistent serology. Avian Pathology 1998 27; 352-358.
Rima B, Alexander DJ, Billeter MA, Collins PL, Kingsbury DW, Lipkind MA, Nagai Y, Orvell C, Pringle CR, TerMeulen V. Paramyxoviridae. In: F.A. Murphy, C.M. Fauquet, D.H.L. Bishop, S.A. Ghabrial, A.W. Jarvis, G.P Martelli, M.A. Mayo and M.D. Summers (Eds.), Virus Taxonomy, Sixth Report of the International Committee on Taxonomy of Viruses 1995;268-274. Springer-Verlag, Wien.
Alexander DJ. Newcastle disease In: Y. M. Saif., J.R. Barnes, A.M. Glisson, L.R. McDougald Fadly and D.E. Swayn (Eds.), Diseases of Poultry (11th edn.) 2003;101-119. Iowa State University Press: Ames.
Kaleta EF, Baldauf C. Newcastle disease in free-living and pet birds. In: DJ Alexander (Ed.), Newcastle Disease 1998; 197-246. Kluwer Academia Publishers, Boston, USA.
Mayahi M, Talazadeh F, Abdolshah M. Effect of genetic strains (Ross 308, Cobb 500 and Hubbard F15) on immune response against Newcastle disease vaccine in broiler chickens. International Journal of Enteric Pathogens 2016; 4(4): e37108. doi: 10.15171/ijep.2016.18.
Cubas ZS. Natural diseases of free- ranging birds in South America. In: M.E. Fowler (Ed.), Zoo and wild animal medicine, current therapy 1993;3: 166-172. W.B. Saunders Company, USA
Higgins DA. Nine disease outbreaks associated with myxoviruses among ducks in Hong Kong. Tropical Animmal Health and Production 1971; 3: 232-240.
Cole RK and Huts FB. Genetic differences in resistance to Newcastle disease. Avian Diseases 1961; 5: 205-214.
Abshar N, Aghakhan SN, Marounesi Ch, Sami Z, Rasoul Nejad Fereidouni S, Khodashenas M, Pourbakhsh SA. Study of quail bronchitis virus (serotype 1, avian adenovirus) isolated from quails. Pajouhesh & Sazandegi 2000; 48: 128-134 (In Persian).
Talazadeh F, Mayahi M. Aquatic birds Surveillance for Newcastle Disease Virus (NDV) in Khozestan province. International Journal of Enteric Pathogens 2013; 1 (1): DOI: 10.5812/IJE.9320.
Alexander DJ. The epidemiology and control of avian influenza and Newcastle disease. Journal of Comparative Pathology 1995; 112: 105-126.
Alexander DJ, Jones RC. Paramyxoviridae. In: F Jordan, M Pattison, D Alexander and T Faragher (Eds.), Poultry Diseases (5th edn.). 2002; 257-280. WB Saunders, UK.
CEC. Council Directive 92/66/EEC of 14 July 1992 introducing Community measures for the control of Newcastle disease. Office Journal of the European Commission 1992; 1-20. European Union (EU).
Alexander DJ, Allan WH. Newcastle disease virus pathotypes. Avian Pathology 1974; 3: 269-278.
Olav SL, Hartog L, Koch G, Peeters BPH. Effect of fusion protein cleavage site mutations on virulence of Newcastle disease virus: non-virulent cleavage site mutants revert to virulence after one passage in chicken brain. Journal of General Virology 2003; 84: 475-484.
Maiorano G, Knaga S, Witkowski A, Cianciullo D, Bednarczyk M. Cholesterol contentmand intramuscular collagen properties of pectoralis superficialis muscle of quail from different genetic groups. Poultry Science 2011; 90: 1620–1626. DOI: 10.3382/ps.2010-01190
Vali N. The Japanese Quail: A Review. International Journal of Poultry Science 2008; 7: 925-931.
Minvielle F. Genetics and breeding of Japanese quail for production around the world. Proceedings 6th Asian Pacific Poultry Congress. Nagoya, Japan 1998; 122-127.
Villegas P. Titration of biologicacal suspensions. In: Dufor-Zavala L, Swayne DE, Glison JR, Pearson JE, Reed MW, Woolcock PR, editors. A laboratory manual for the isolation, identifi cation and characterization of avian pathogens. 5th ed. Athene, GA: American Association of Avian Pathologist 2008.
Talazadeh F, Varnaseri S, Mayahi M, Gharibi D, Mohammadian T. The effect of Antibiofin® on lactic acid bacteria (LAB)population and the Lactobacillus numbers of digestigve system in broiler chickens. Iranian Veterinary Journal 2016; (13) 3 :67-74. 10.22055/ivj.2017.53536.1728 (In Persian).
Talazadeh F, Naghavi M, Mayahi M, Gharibi D. The effect of Antibiofin® on intestinal bacterial population in broiler chickens. Journal of Zoonotic Diseases 2017 ; 2 (1) : 9-13.
Gong J, Si W, Forster RJ, Huang R, Yu H, Yin Y et al. 16S rRNA gene-based analysis of mucosa-associated bacterial community and phylogeny in the chicken. FEMS Microbiology Ecology 2007; 59:147–157. doi: 10.1111/j.1574-6941. 2006.00193.x
Turmagambetova AS, Alexyuk MS, Bogoyavlenskiy AP, Linster M, Alexyuk PG, Zaitceva IA et al. Monitoring of Newcastle disease virus in environmental samples. Archives of Virology 2017; 162:2843–2846. doi: 10.1007/ s00705-017-3433-y
Li YP. ERIC-PCR Based Fingerprint to Analyze the Structural Features of IntestinalMicrobial Communities of Chicks Infected with Salmonella gallinarum, Newcastle Disease Virus and the Healthy Chicks. Ph.D. thesis, Sichuan Agricultural University, Sichuan 2007.
Momayez R, Gharahkhani P, Pourbaksh SA, Toroghi R, Shoushtari AH, Banai M. Isolation and pathogenicity identifi cation of avian paramyxovirus serotype 1 (Newcastle disease) virus from a Japanese quail flock in Iran. Archives of Razi Institute 2007; 62 (1):39-44.
Ghiamirad M, Pourbakhsh A, Keyvanfar H, Momayaz R, Charkhkar S, Ashtari A. Isolation and characterization of Newcastle disease virus from ostriches in Iran. African journal of microbiology research 2010; 4 (23): 2492-7.
Madadgar O, Karimi V, Nazaktabar A, Kazemimanesh M, Ghafari MM, Azimi Dezfouli SM. A study of Newcastle disease virus obtained from exotic caged birds in Tehran between 2009 and 2010. Avian Pathology 2013; 42 (1) : 27-31.
Mehrabanpour MJ, Khoobyar S, Rahimian A, Nazari MB, Keshtkar MR. Phylogenetic characterization of the fusion genes of the Newcastle disease viruses isolated in Fars province poultry farms during 2009-2011. Veterinary Research Forum 2014 ; 5 (5) :187-91.
Cui N, Huang X, Kong Z, Huang Y, Huang Q, Yang S, Zhang L, Xu C, Zhang X, Cui Y. Newcastle Disease Virus Infection Interferes With the Formation of Intestinal Microflora in Newly Hatched Specific-Pathogen-Free Chicks. Frontiers in Microbiology 2018;9:900. doi: 10.3389/fmicb.2018.00900.
Zhou Z, Nie K, Huang Q, Li K, Sun Y, Zhou R et al. Changes of cecal microflora in chickens following Eimeria tenella challenge and regulating effect of coated sodium butyrate. Experimental Parasitology 2017; 177: 73–81. doi: 10.1016/j.exppara. 2017.04.007.
Perumbakkam S, Hunt HD, Cheng HH. Marek’s disease virus influences the core gut microbiome of the chicken during the early and late phases of viral replication. FEMS Microbiology Ecology 2014; 90: 300–312. doi: 10.1111/ 1574-6941.12392.
Gomez de Agüero M, Ganal-Vonarburg SC, Fuhrer T, Rupp S, Uchimura Y, Li H et al. The maternal microbiota drives early postnatal innate immune development. Science 2016; 351: 1296–1302. doi: 10.1126/science.aad2571
Günther C, Josenhans C, Wehkamp J. Crosstalk between microbiota, pathogens and the innate immune responses. International Journal of Medical Microbiology 2016; 306: 257–265. doi: 10.1016/j.ijmm.2016.03.003
Zhang LJ, Wang GQ, Yu HL, Wang J, Wang SW, Jia Y et al. First report of human infection by Rhodoplanes sp. Alphaproteobacteria in China. Asian Pacific Journal of Tropical Medicine 2011; 4: 248–250. doi: 10.1016/S1995-7645(11)60079-5
Girardin H, Albagnac C, Dargaignaratz C, Nguyen-The C, Carlin F. Antimicrobial activity of foodborne Paenibacillus and Bacillus spp. against Clostridium botulinum. Journal of Food Protection 2002; 65: 806–813. doi: 10.4315/0362- 028X-65.5.806
Passera A, Venturini G, Battelli G, Casati P, Penaca F, Quaglino F et al. Competition assays revealed Paenibacillus pasadenensis strain R16 as a novel antifungal agent. Microbiological Research 2017; 198: 16–26. doi: 10.1016/j.micres.2017. 02.001
Hugh R, Huang KY, Elliott TB. Enhancement of bacterial infections in mice by Newcastle disease virus. Infection and Immunity 1971; 3: 488–493.
Chang C, Lin H. Dysbiosis in gastrointestinal disorders. Best Practice & Research Clinical Gastroenterology 2016; 30 : 3–15. doi: 10.1016/j.bpg.2016.02.001
Hunt PW, Landay AL, Sinclair E, Martinson JA, Hatano H, Emu B et al. A low T regulatory cell response may contribute to both viral control and generalized immune activation in HIV controllers. PLoS One 2011; 31:e15924. doi: 10.1371/journal.pone.0015924
Marchetti G, Cozzi-Lepri A, Merlini E, Bellistrì GM, Castagna A, Galli M et al. Microbial translocation predicts disease progression of HIVinfected antiretroviral-naive patients with high CD4C cell count. AIDS 2011; 25: 1385–1394. doi: 10.1097/QAD.0b013e3283471d10
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