Does supplementation of boron with yeast or humate substances have a better chance at modulating rumen fermentation in yearling rams?

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Published: Nov 9, 2022
Updated: 2022-11-09
DOI: https://doi.org/10.12681/jhvms.26454
Keywords:
Merino rams rumen fermentation boron Saccharomyces cerevisiae humate
O Sizmaz
Ankara University
https://orcid.org/0000-0002-2027-5074
BH Koksal
Adnan Menderes University
https://orcid.org/0000-0002-5676-446X
MS Ramay
Ankara University
https://orcid.org/0000-0002-7061-0483
G Yildiz
Ankara University
https://orcid.org/0000-0002-1003-9254
Abstract

This study aimed to determine the effects of boron fed alone or with yeast culture or liquid humate on ruminal pH, ammonia-N, short-chain fatty acid (SCFA) concentration, protozoa counts and some nutrients’ apparent digestibility in yearling rams. Therefore, four Merino rams were allocated to 4×4 Latin square design and were randomly assigned to one of four treatments to basal diet: negative control with no supplements (NC), positive control (PC) with 30 ppm (Dry matter basis) boric acid alone, combinations of 30 ppm boric acid and 3 g/kg yeast culture (YC) and 30 ppm boric acid and 5 ml/kg liquid humate (LH). There were no negative effects of additives’ supplementation on animals daily feed consumption. No significant differences were observed among treatments for rumen parameters investigated, however, numerically lowest values for pH & ammonia-N in LH while highest SCFA concentrations in YC and LH groups were observed. Apparent nutrient digestibility was altered with boron addition to diets. Boron analysis of blood serum and fecal samples showed non-significant changes, except higher serum concentration of boron in YC compared to PC. It can be concluded that tested dosage of boron was not enough to considerably modify rumen fermentation characteristics in yearling rams. Better modulation of rumen fermentative characteristics might be possible when boron is supplemented with yeast or liquid humate, however, this possibility should be verified in the large-scale experiment.

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Author Biography
MS Ramay, Ankara University

Teaching Assistant

Department of Animal Nutrition and Nutritional Diseases

References
AOAC (2000) Official Methods of Analysis. Association of Official Analytical Chemists, Washington, DC.
Bennett A, Rowe RI, Soch N, Eckhert CD (1999) Boron stimulates yeast (Saccharomyces cerevisiae) growth. J Nutr 129:2236-2238.
Bharti VK, Gupta M, Lall D (2007) Effects of boron on haemogram and biochemical profile of urine in buffalo calves fed a high fluoride ration. Fluoride 40:238.
Bharti VK, Gupta M, Lall D (2008) Effect of boron as an antidote on dry matter intake, nutrient utilization and fluorine balance in buffalo (Bubalus bubalis) exposed to high fluoride ration. Biol Trace Elem Res 126(1):31-43.
Bhatta R, Tajima K, Kurihara M (2006) Influence of temperature and pH on fermentation pattern and methane production in the rumen simulating fermenter (RUSITEC). Asian-Australas J Anim Sci 19:376-380.
Bolanos L, Lukaszewski K, Bonilla I (2014) Why boron? Plant Physiol. Biochem 42:907–912.
Chaucheyras-Durand F, Durand H (2010) Probiotics in animal nutrition and health. Benef microbes 1:3-9.
Chirase NK, Greene LW, McCollum FT, Auvermann BW, Cole NA (2000) Effects of bovipro on performance and serum metabolites concentrations of beef steers. In: Proceedings of the American Society of Animal Science West Section: pp 51.
Cox C. Boric acids and borates (2004) Journal of Pesticide Reform 24:10-15.
Denev SA, Peeva TZ, Radulova P, et al. (2007) Yeast cultures in ruminant nutrition. Bulg J Agric Sci 13:357-374.
Devirian TA, Volpe SL (2003) The physiological effects of dietary boron. Crit Rev Food Sci Nutr 43:219-231.
El-Shazly K (1952) Degradation of protein in the rumen of the sheep. 1. Some volatile fatty acids, including branched-chain isomers, found in vivo. Biochem J 51:640-647.
El-Zaiat HM, Morsy AS, El-Wakeel EA, Anwer MM, Sallam SM (2018) Impact of humic acid as an organic additive on ruminal fermentation constituents, blood parameters and milk production in goats and their kids growth rate. J Anim Feed Sci 27:105-113.
Fry RS, Lloyd KE, Jacobi SK, Siciliano PD, Robarge WP, Spears JW (2010) Effect of dietary boron on immune function in growing beef steers. J Anim Physiol Anim Nutr 94:273-279.
Galip N, Polat U, Biricik H (2010) Effects of supplemental humic acid on ruminal fermentation and blood variables in rams. Ital J Anim Sci 9:74.
Geiissler CH, Hoffmann M, Hickel B (1976) A contribution to the gas chromatographic determination of volatile fatty acids. Arch Anim Nutr 26:123–129.
Guedes CM, Goncalves D, Rodrigues MAM, Dias-da-Silva A (2008) Effects of a Saccharomyces cerevisiae yeast on ruminal fermentation and fibre degradation of maize silages in cows. Anim Feed Sci Technol 145:27-40.
Herdt TH (1988) Fuel homeostasis in the ruminant. Vet Clin N Am Food A 4:213-231.
Ji F, McGlone JJ, Kim SW (2006) Effects of dietary humic substances on pig growth performance, carcass characteristics, and ammonia emission. J Anim Sci 84:2482–2490.
Kabu M, Civelek T (2012) Effects of propylene glycol, methionine and sodium borate on metabolic profile in dairy cattle during periparturient period. Revue Med Vet 163:419-430.
Lana RP, Russell JB, Van Amburgh ME (1998) The role of pH in regulating ruminal methane and ammonia production. J Anim Sci 76:2190-2196.
McMurphy CP, Duff GC, Sanders SR, Cuneo SP, Chirase NK (2011) Effects of supplementing humates on rumen fermentation in Holstein steers. S Afr J Anim Sci 41:134-140.
Ogimoto K, Imai S (1981) Atlas of Rumen Microbiology. Japan Scientific Societies Press. Tokyo (Japan) 158.
Sena JA, Villela SD, Santos RA, et al. (2015) Intake, digestibility, performance, and carcass traits of rams provided with dehydrated passion fruit (Passiflora edulis f. flavicarpa) peel, as a substitute of Tifton 85 (Cynodon spp.). Small Ruminant Res 129:18-24.
Seo JK, Kim SW, Kim MH, Upadhaya SD, Kam DK, Ha JK (2010) Direct-fed microbials for ruminant animals. Asian-Australas J Anim Sci 23:1657-1667.
Shi Y, Parker DB, Cole NA, Auvermann BW, Mehlhorn JE (2001) Surface amendments to minimize ammonia emissions from beef cattle feedlots. Transactions of the ASAE 44:677-682.
Sizmaz O, Koksal BH, Yildiz G (2017) Rumen microbial fermentation, protozoan abundance, and boron availability in yearling rams fed diets with different boron concentrations. J Anim Feed Sci 26:59-64.
Sizmaz O, Koksal BH, Yildiz G (2019) Investigating of usage of boric acid in yearling rams. In: Proceedings of 1st Livestock Science Congress, Antalya, Turkey.
Stevenson FJ (2010) Humus Chemistry: Genesis, Composition, Reactions. John Wiley & Sons, New York.
TSE 1994 Animal Feeds. Metabolic Energy Determination (Chemical method). Turkish Standards Institute No: 9610, Ankara.
Vanatta LE, Coleman DE, Slingsby RW (1999) Low-level calibration study for a new ion chromatographic column to determine borate in deionized water. J Chromotogr A 850:107–117.
Varadyova Z, Kisidayova S, Jalč D (2009) Effect of humic acid on fermentation and ciliate protozoan population in rumen fluid of sheep in vitro. J Sci Food Agr 89:1936-1941.
Xu D, Peak D (2007) Adsorption of boric acid on pure and humic acid coated am-Al (OH) 3: a boron K-edge XANES study. Environ Sci Technol 41:903-908.
Yildiz G, Koksal BH, Sizmaz O (2011) Effects of dietary boric acid and yeast (Saccharomyces cerevisiae) supplementation on performance, carcass traits and some blood parameters of broilers. Kafkas Univ Vet Fak 17:429-434.
Yildiz G, Koksal BH, Sizmaz O (2013) Effects of dietary boric acid addition on growth performance, cholesterolemia, some carcass and tibia characteristics in different rearing periods in broiler chickens. Revue Med Vet 164:219-224.
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