Detection of Mycotoxigenic Fungi and Mycotoxins in Poultry Feed of Balochistan Pakistan

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DOI: https://doi.org/10.12681/jhvms.26759
Keywords:
Aspergillus Aflatoxins Fumonisin Chromatography ELISA
FA MAJEED
Department of Microbiology, University of Balochistan Quetta, Pakistan
A AKBAR
Department of Microbiology, University of Balochistan Quetta, Pakistan
M SHAFEE
Centre for Advanced studies in Vaccinology and Biotechnology, University of Balochistan Quetta, Pakistan
H SADIA
Department of Microbiology Balochistan University of Information Technology, Engineering and Management Sciences, Quetta, Pakistan
S IQBAL
Department of Chemistry,Balochistan University of Information Technology, Engineering and Management Sciences, Quetta, Pakistan
B PHADUNGCHOB
General Science Department Faculty of Education, Dhonburi Rajabhat University Bangkok Thailand
GI KHAN
Department of Microbiology, University of Balochistan Quetta, Pakistan
SA KHAN
Department of Microbiology, University of Balochistan Quetta, Pakistan
Abstract

Mycotoxins are secondary toxic metabolites of fungi, commonly associated to agriculture commodities. Aspergillus, Fusarium, Penicillium and Claviceps genera are the most common mycotoxigenic fungi and the most important types of mycotoxin are Aflatoxins, Zearalenone, Ochratoxin, Fumonisins and Trichothecenes Mycotoxins can be carcinogenic, cytotoxic, mutagenic, teratogenic, neurotoxic, nephrotoxic, estrogenic and immunosuppressant. This study was conducted to examine the presence of mycotoxigenic fungi and mycotoxins in poultry feed used by the poultry farmers of Balochistan. Samples (n=100) were analyzed and found contaminated with fungi such as Aspergillus, Mucor, Rhizopus and Fusarium genera, amongst them Aspergillus species was observed highly prevalent (89%). Aflatoxin production in culture plates were initially screened with the help of ammonia hydroxide vapor test, and subsequently analyzed with the help of AgraStrip®. The Aflatoxins (77%) and Fumonisins (92%) were found in direct examination of feed samples. Toxins presence was also confirmed with the help of ELISA using AgraQuant® kits. Thin Layer Chromatography (TLC) and High-Performance Liquid Chromatography (HPLC) were used for characterization and quantification of aflatoxins. It was concluded that the feed samples were abundantly contaminated with aflatoxins and fumonisins and the fungi responsible for their production. This contaminated feed is one of the reasons for economic lossesfor poultry farmers of the region.

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References
Ahmed DA, Al-Khafaji NJ, Ahmed LT(2017) Isolation and molecular identification of Aspergillus spp. collected from different sources of animals feed. Int J Curr Microbiol App Sci6(6):1792-1797.
Ali FS, Akbar A,Prasongsuk S, Permpornsakul P, Yanwisetpakdee B, Lotrakul P, Punnapayak H, Asrar M, Ali I(2018) Penicillium Imranianum, A new species from the man-made solar saltern of Phetchaburi province, Thailand. Pak J Bot 50(5):2055-2058.
Alvarado-Hernández JR, Carvajal-Moreno M, Rojo-Callejas F, Ruiz-Velasco S(2016) Aflatoxins in natural peanuts (Arachishypogaea L.) of Mexico: Validation of the biochemical methods for extraction and quantification. J Plant BiochemPhysiol 4:1000168.
Anjum MA, Khan SH, Sahota AW, Sardar R(2012) Assessment of aflatoxin B1 in commercial poultry feed and feed ingredients. J Anim Plant Sci 21: 117-120.
Battilani P, Pietri A, Bertuzzi T, Languasco L, Giorni P,Kozakiewicz Z(2003) Occurrence of ochratoxin A–producing fungi in grapes grown in Italy. J Food Prot 66(4):633-636.
BeyeneAM, Du XE, Schrunk D, Steve E,Rumbeiha KW(2019) High-performance liquid chromatography and Enzyme-Linked Immunosorbent Assay techniques for detection and quantification of aflatoxin B1 in feed samples: a comparative study. BMC Res Notes 12: 492.https://doi.org/10.1186/s13104-019-4538-z
Capriotti AL, Caruso G, Cavaliere C, Foglia P, Samperi R,LaganàA(2012) Multiclass mycotoxin analysis in food, environmental and biological matrices with chromatography/ mass spectrometry. Mass Spectr Rev 31(4):466-503.
Dalcero A, Magnoli C, Chiacchiera S, Palacios G, Reynoso M(1997)Mycoflora and incidence of aflatoxin B1, zearalenone and deoxynivalenol in poultry feeds in Argentina. Mycopathologia 137(3):179-184.
da Rocha EBM, Freire FCO, Maia FEF, Guedes FIF, Rondina D (2014). Mycotoxins and their effects on human and animal health. Food Cont 36(1):159-165. DOI: http://doi.org/10.1016/j.foodcont.2013.08.021
FAO (2002) The State of Food Insecurity in the World (SOFI), Food and Agriculture Organization of the United Nations. http://www.fao.org/docrep/005/y7352e/y7352e0 0.htm.
Flores-Flores ME, González-Peñas E(2018) Analysis of mycotoxins in Spanish milk. J Dairy Sci 101:113–117.
Gherbawy YA, Shebany YM,Alharthy H (2019) Aspergilli and Their Aflatoxins Contamination of Poultry and Animal Feedstuff Samples in Western Region of Saudi Arabia. Sains Malaysiana48(4):765-771.
Greco MV, Franchi ML, Rico Golba SL, Pardo AG, Pose GN(2014) Mycotoxins and mycotoxigenic fungi in poultry feed for food-producing animals. Sci World J 2014.
Jajić I, Glamočić D, Krstović S, PolovinskiHorvatović M (2018) Aflatoxin M1 occurrence in Serbian milk and its impact on legislative. J Hellenic Vet Med Soc 69(4):1283-1290.
KanaRK, Gnonlonfin BGJ, Harvey J, Wainaina J, Wanjuki I, SkiltonRA, Teguia A(2013) Mycobiota and toxigenicity profile of Aspergillus flavus recovered from food and poultry feed mixtures in cameroon. J Ani Poult Sci 2(4):98-107.
Khan GI, Akbar A, Anwar M, Shafee M, Sadia H, Razzaq A, Rehman FU (2020) Eco-friendly bacterial biodegradation of mycotoxins. Pure Appl Biol 9(2):1708-1717. http://dx.doi.org/10.19045/bspab.2020.90181
Kos G, Markus S, David M, Celine Z, Michael S, Tuba O, Boris M, Rudolf K(2016) A novel chemometric classification for FTIR spectra of mycotoxin-contaminated maize and peanuts at regulatory limits. Food AdditCont: Part A33:10:1596-1607. DOI:10.1080/19440049.2016.1217567
Mirza SN, Athar M, Qayyum M (2009) Eff ect of Drought on Rangeland Productivity and Animal Performance in Dryland Region of Balochistan, Pakistan. Agricul Conspectus Sci 74(2):105-109.
Osho IB, Awoniyi TAM, Adebayo AI(2007) Mycological investigation of compounded poultry feeds used in poultry farms in southwest Nigeria. Afri J Biotech 6(15).
Pacin AM, Gonzalez HHL, Etcheverry M, Resnik SL, Vivas L,Espin S(2003) Fungi associated with food and feed commodities from Ecuador. Mycopathologia 156(2):87-92.
Rafique A, Javed MT, Ali SS(2018) Contamination of commercial poultry feeds and feed ingredients with Aspergillus flavus and aflatoxin B1 in Punjab Pakistan. J Ani Plant Sci 28(4):1021-1026.
Raju S, PadamadanCJ,Shenpagam NH(2016) Mycotoxin production by fungi isolated from commercially prepared livestock feed in Kerala. Int J Appl Res2(5):154-159.
Rashid N, Bajwa A, Rafeeq M, Khan MA, Ahmad Z, Tariq MM, Wadood A, Abbas F(2012) Prevalence of aflatoxin B1 in finished commercial broiler feed from west central Pakistan. J Anim Plant Sci 22: 6-10.
Rosa CDR, Ribeiro JMM, Fraga MJ, Gatti M, Cavaglieri LR, Magnoli CE, Lopes CWG(2006) Mycoflora of poultry feeds and ochratoxin-producing ability of isolated Aspergillus and Penicillium species. Vet Microbiol 113(1-2):89-96.
Saleemi MK, Khan MZ, Khan A, Javed I, Hasan ZU, Hameed MR, Mehmood MA (2012) Occurrence of toxigenic fungi in maize and maize-gluten meal from Pakistan. PhytopathologiaMediterranea 51(1).
Sana S, Anjum AA, Yaqub T, Nasir M, Ali MA, Abbas M(2019) Molecular approaches for characterization of aflatoxin producing Aspergillus flavus isolates from poultry feed. Pak Vet J 39(2):169-174.
Shekhar M, Singh N, Dutta R, Kumar S, Mahajan V (2017) Comparative study of qualitative and quantitative methods to determine toxicity level of Aspergillus flavus isolates in maize. PLoS ONE 12(12):e0189760. https://doi.org/10.1371/journal. pone.0189760
Shi H, ShengliL, Yueyu B, Luciana LP, Yaogeng L,Peiqiang Y (2018) Mycotoxin contamination of food and feed in china: occurrence, detection techniques, toxicological effects and advances in mitigation technologies. Food Cont 91, 202–215.
Sieger, Gregor MK, Michael S,Matthia G, Rudolf K, Boris M(2017) Portable Infrared Laser Spectroscopy for On-site Mycotoxin Analysis. Sci Rep 7:44028. doi: 10.1038/srep44028.
Yazdani D, Zainal AM, Tan YH,Kamaruzaman S(2010) Evaluation of the detection techniques of toxigenic Aspergillus isolates. Afri J Biotech 9(45):7654-7659.
Wacoo AP, Wendiro D, Vuzi PC, Hawumba JF (2014) Methods for detection of aflatoxins in agricultural food crops. J Appl Chem 2014.