Sarcocystis sinensis in slaughtered cattle from Central of Iran


Published: Jul 10, 2022
Keywords:
Sarcocystis Cattle Food Safety
B Hajimohammadi
https://orcid.org/0000-0003-4463-5465
G Eslami
https://orcid.org/0000-0002-4521-4059
L Manafi
https://orcid.org/0000-0002-9124-8815
SS Athari
https://orcid.org/0000-0002-6355-6378
MJ Boozhmehrani
https://orcid.org/0000-0003-2497-6158
Abstract

Sarcocystis is a food borne parasite as an intracellular parasite with two-host life cycle. Humans serve as either intermediate or definitive hosts among different species. Sarcocystosis is a very important disease because of its widespread prevalence with an impact on production and animal health. We aimed to assess S. sinensis in cattle from the desert area of Iran, using conventional PCR, targeting 18S rRNA. Also, the phylogenic analysis was done to mention isolate and the related ones in Genbank.

From the 108 beef samples of heart, esophagus, intercostal muscle, diaphragm, and tongue harboring Sarcocystis spp. stored in the BioBank of the Research Center for Health and Food Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran, DNA extraction was done. Amplification was done using the primer pair of SAR-F: 5'-TGGCTAATACATGCGCAAATA-3' and SAR-R: 5'-AACTTGAATGATCTATCGCCA-3'

For the target of 18S rRNA gene. Sequencing was done. The interesting sequence was edited and then analyzed with BLAST. Then, multiple alignments were conducted using T-coffee. The mentioned sequence was submitted to GenBank in NCBI. Evolutionary analyses were conducted in MEGA7. In order to statistical analysis of the presence of Sarcocystis sinensis significantly with sex, age, and location, the Fisher’s Exact test was done using SPSS 16.0 (SPSS Inc. Chicago, USA). The p-value < 0.05 was considered significant.

Out of 108 samples, five isolates were identified as S. sinensis. The distribution of S. sinensis was related to age (p<0.05) but had no significant difference with gender (p>0.05). Phylogenic analysis showed a similarity of S. sinensis with S. hominis. From a food safety viewpoint, this study employed a molecular approach to identify S. sinensisin in cattle meat. To the best of our knowledge, this is the first report of S. sinensis in the desert area from Iran.

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References
Bunyaratvej S, Unpunyo P (1992) Combined Sarcocystis and gram positive bacterial infections. A possible cause of segmental enterocolitis in Thailand. J Med Assoc Thai 75:38-44.
Chen X, Zuo Y, Rosenthal BM, He Y, Cui L, Yang Z (2011) Sarcocystis sinensis is an ultrastructurally distinct parasite of water buffalo that can cause foodborne illness but cannot complete its life-cycle in human beings. Vet Parasitol 178:35-39.
Domenis L, Peletto S, Sacchi L, Clementi E, Genchi M, Felisari L, Felisaric C, Mo P, Modesto P, Zuccon F, Campanella C, Maurella C, Guidetti C, Acutis PL (2011) Detection of a morphogenetically novel Sarcocystis hominis-like in the context of a prevalence study in semi-intensively bred cattle in Italy. Parasitol Res 109:1677-1687.
Dubey JP, Moré G, van Wilpe E, Calero-Bernal R, Verma SK, Schares G (2016) Sarcocystis rommeli, n. sp. (Apicomplexa: Sarcocystidae) from Cattle (Bos taurus) and its differentiation from Sarcocystis hominis. J Eukaryot Microbiol 63:62-68.
Dubey JP, Speer CA, Fayer R (1989) Sarcocystosis of animals and man. Boca Raton, FL.: CRC Press, Inc.
Dubey JP, van Wilpe E, Calero-Bernal R, Verma SK, Fayer R (2015) Sarcocystis heydorni, n. sp. (Apicomplexa: Sarcocystidae) with cattle (Bos taurus) and human (Homo sapiens) cycle. Parasitol Res 114:4143-4147.
Eslami G, Hajimohammadi B, Moghadam Ahmadi M, Dehghani A, Zandi H, Hoseinpour Ganjaroudi F, Khalatbari S (2014) Molecular assay for fraud identification of handmade hamburgers. Int J Enteric Pathogens 2:e21152.
Farhang-Pajuh F, Yakhchali M, Mardani K (2014) Molecular determination of abundance of infection with Sarcocystis species in slaughtered sheep of Urmia, Iran. Vet Res Forum, 5:181-186.
Fayer R (2004) Sarcocystis spp. in human infections. Clin Microbiol Rev 17:894-902.
Fischer S, Odening K (1998) Characterization of bovine Sarcocystis species by analysis of their 18S ribosomal DNA sequences. J Parasitol 84:50-54.
Gjerde B (2013) Phylogenetic relationships among Sarcocystis species in cervids, cattle and sheep inferred from the mitochondrial cytochrome coxidase subunit I gene. Int J Parasitol. 43:579-591.
Gjerde B, Hilali M (2016) Domestic cats (Felis catus) are definitive hosts for Sarcocystis sinensis from water buffaloes (Bubalus bubalis). J Vet Med Sci 78:1217-1221.
Hajimohammadi B, Dehghani A, Moghaddam-Ahmadi M, Eslami G, Oryan A, Yasini-Ardakani SA, Zohourtabar A, Mirzaei F (2014a) Isolation of Sarcocystis hirsuta from traditional hamburger of Iran. J Isf Med School 32:79-85.
Hajimohammadi B, Dehghani A, Moghadam-Ahmadi M, Eslami G, Oryan A, Khamesipour A (2014b) Prevalence and species identification of Sarcocystis in raw hamburgers distributed in Yazd, Iran using PCR-RFLP. J Food Qual Hazards Control 1:15-20.
Hajimohammadi B, Eslami G, Oryan A, Zohourtabar A, Pourmirzaei Tafti H, Moghaddam Ahmadi M (2014c) Molecular identification of Sarcocystis hominis in native cattle of central Iran: a case report. Trop Biomed 31:183-186.
Hajimohammadi B, Eslami G, Zohourtabar A, Dehghani A, Oryan A, Pourmirzaei Tafti H, Radouani F (2014d) High occurrence of Sarcocystis cysts in meat produced in Yazd, central Iran. J Food Qual Hazards Control 1:95-101.
Hamidinejat H, Razi Jalali MH, Nabavi L (2010) Survey on Sarcocystis infection in slaughtered cattle in South-West of Iran, emphasized on evaluation of muscle squash in comparison with digestion method. J Anim Vet Adv 9:1724-1726.
Heckeroth A, Tenter AM (2007) Sarcocystiosis. In L.M. Ortega-Mora, B. Gottstein, F.J. Conraths, D. Buxton (Eds), Protozoal Abortion Infarm Ruminants. United Kingdom: CABI, GatesheadP: 172-232.
Hooshyar H, Abbaszadeh Z, Sharafati-Chaleshtori R, Arbabi M (2017) Molecular identification of Sarcocystis species in raw hamburgers using PCR-RFLP method in Kashan, central Iran. J Parasit Dis 41:1001-1005.
Hornok S, Mester A, Takács N, Baska F, Majoros G, Fok E, Biksi I, Német Z, Hornyák A, Jánosi S, Farkas R (2015) Sarcocystis-infection of cattle in Hungary. Parasit Vectors 8:69.
Huong LT, Uggla A (1999) Sarcocystis dubeyi n. sp. (Protozoa: Sarcocystidae) in the water buffalo (Bubalus bubalis). J Parasitol 85:102-104.
Li QQ, Yang Z, Zuo YX, Attwood SW, Chen XW, Zhang YP (2002) PCR-based RFLP analysis of Sarcocystis cruzi (protozoa: sarcocystidae) in Yunnan province, PR China, reveals the water buffalo (Bubalus bubalis) as a natural intermediate. J Parasitol 88:1259-1261.
Mirzaei M, Rezaei H (2016) A survey on Sarcocystis spp. infection in cattle of Tabriz city, Iran. J Parasit Dis 40:648-651.
Moghaddam Ahmadi M, Hajimohammadi B, Eslami G, Oryan A, Yasini Ardakani SA, Zohourtabar A, Zare S (2015)First identification of Sarcocystis hominis in Iranian traditional hamburger. J Parasit Dis 39:770-772.
Moré G, Basso W, Bacigalupe D, Venturini MC, Venturini L (2008) Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle. J Parasitol Res 102:671-675.
Moré G, Pantchev A, Skuballa J, Langenmayer MC, Maksimov P, Conraths FJ, Venturini MC, Schares G (2014) Sarcocystis sinensis is the most prevalent thick-walled Sarcocystis species in beef on sale for consumers in Germany. J Parasitol Res 113:2223-2230.
Moré G, Schares S, Maksimov A, Conraths FJ, Venturini MC, Schares G (2013) Development of a multiplex real time PCR to differentiate Sarcocystis spp. affecting cattle. Vet Parasitol 197:85-94.
Notredame C, Higgins DG, Heringa J (2000) T-Coffee: A novel method for fast and accurate multiple sequence alignment. J Mol Biol 302:205-217.
Obijiaku IN, Ajogi I, Umoh JU, Lawal IA, Atu BO (2013). Sarcocystis infection in slaughtered cattle in Zango abattoir, Zaria, Nigeria. Vet World 6:346-349.
Oryan A, Moghaddar N, Gaur SN (1996) The distribution pattern of Sarcocystis species, their transmission and pathogenesis in sheep in Fars province of Iran. Vet Res Commun 20:243-253.
Regensburger C, Gos M, Ctibor J, Moré G (2015) Morphological and molecular characteristics of Sarcocystis aucheniae isolated from meat of guanaco (Lama guanicoe). J Food Qual Hazards Control 2:118-121.
Savini G, Dunsmore JD, Robertson ID, Seneviratna P (1992) The epidemiology of Sarcocystis spp. in cattle of Western Australia. Epidemiol Infect 108:107-113.
Shahraki MK, Ghanbarzehi A, Dabirzadeh M (2018) Prevalence and histopathology of Sarcocystosis in slaughtered carcasses in southeast Iran. J Adv Vet Anim Res 5:381-387.
Stojecki K, Karamon J, Sroka J, Cencek, T (2012) Molecular diagnostics of Sarcocystis spp. infections. Pol J Vet Sci 15:589-596.
Vangeel L, Houf K, Chiers K, Vercruysse J, D'Herde K, Ducatelle R (2007) Molecular-based identification of Sarcocystis hominis in Belgian minced beef. J Food Prot 70:1523-1526.
Yang ZQ, Zuo YX, Ding B, Chen XW, Luo J, Zhang YP (2001a)Identification of Sarcocystis hominis-like (Protozoa: Sarcocystidae) cyst in water buffalo (Bubalus bubalis) based on 18S rRNA gene sequences. J Parasitol 87:934-937.
Yang ZQ, Zuo YX, Yao YG, Chen XW, Yang GC, Zhang YP (2001b) Analysis of the 18S rRNA genes of Sarcocystis species suggests that the morphologically similar organ from cattle and water buffalo should be considered the same species. Mol Biochem Parasitol 115:283-288.
Zhang Z, Schwartz S, Wagner L, Miller W (2000) A greedy algorithm for aligning DNA sequences. J Comput Biol 7:203-214.
Zuo YX (1992) Coccidians in livestock and birds and human coccidiosis. Tianjin: Science and Technology Publishing Company of Tianjin pp: 356-357.