The potential status of A1 and A2 variants of bovine beta-casein locus of some indigenous genetic resources reared in Turkey

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Published: Jul 10, 2022
DOI: https://doi.org/10.12681/jhvms.27018
Keywords:
A1 Allele A2 Allele Beta-Casein PCR-RFLP TaqI Polymorphism
Özcan Şahin
Selçuk University
https://orcid.org/0000-0003-2170-2055
Saim Boztepe
Selçuk University
https://orcid.org/0000-0003-1096-9141
Abstract

The aim of this study was to determine the Beta-Casein gene (CSN2) polymorphism in four domestic cattle breed. The genomic DNA of 374 animals, including Anatolian Black (AB, n= 100), Eastern Anatolian Red (EAR, n= 100), Southern Anatolian Red (SAR, n= 87), Turkish Grey (TG, n= 87), were obtained, and C>A polymorphism in 67th amino acid in the 7th exons of β-casein gene was determined by TaqI enzyme with PCR-RFLP with method. The A1 allele frequency were determined in AB, EAR, SAR and TG as 0.200, 0.195, 0.190 and 0.201, respectively, while the A2 allele frequency were 0.800, 0.805, 0.810 and 0.799, respectively. A1 and A2 allele frequencies were generally calculated as 0.200 and 0.800, respectively. A1A1 genotypes in AB, EAR, SAR and TG breeds were 0.020, 0.070, 0.000 and 0.020, respectively; A1A2 genotypes were 0.360, 0.250, 0.380 and 0.360, respectively; A2A2 genotypes were determined as 0.620, 0.680, 0.620 and 0.620, respectively. The A1A1 genotype frequency has not been detected in the SAR breed.  AB, EAR, SAR and TG cattle breeds were calculated as 0.360, 0.250, 0.379 and 0.356 for Ho; 0.320, 0.314, 0.307 and 0.321 for He and 0.380, 0.320, 0.379 and 0.379 for PIC, respectively. EAR and SAR population were not found in Hardy-Weinberg equilibrium (P<0.05). However, AB and TG populations were found in Hardy-Weinberg equilibrium in terms of β-casein gene (P> 0.05).

In conclusion, AB, EAR, SAR and TG cattle breeds having A2A2 genotype commonly reared in Turkey are satisfactory and could be used in selection programs as a domestic genetic resource for A2 milk production in the future.

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Author Biography
Özcan Şahin, Selçuk University

Dr.

References
Anonymous (2020) Turkey Statistical Institute https://biruni.tuik.gov.tr Access Date: 21/01/2020.
Anderson J. A, Churchill G. A, Autrique J. E, Tanksley S. D, Sorrells M. E (1993) Optimizing parental selection for genetic linkage maps. Genome 36: 181-186.
Aytekin I, Ozdil F, Zulkadir U, Boztepe S, Sariyel V (2011) Evaluation of ISSR markers for genetic diversity analysis in Anatolian water buffaloes. Journal of the Science of Food and Agriculture 91: 1957-1962.
Botstein D, White RL, Skolnick M, Davis, RW. (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American journal of human genetics, 32(3), 314-331.
Caroli A, Chessa S, Erhardt G (2009) Invited review: Milk protein polymorphisms in cattle: Effect on animal breeding and human nutrition. Journal of dairy science 92: 5335-5352.
Cieślińska A, Fiedorowicz E, Zwierzchowski G, Kordulewska N, Jarmołowska B, Kostyra, E (2019) Genetic Polymorphism of β-Casein Gene in Polish Red Cattle—Preliminary Study of A1 and A2 Frequency in Genetic Conservation Herd. Animals 9: 377.
Dar AH, Kumar S, Kumari P, Mukesh M, Singh D, Sharma R, Ghosh A, Singh B, Panwar VA, Sodhi M (2018) Distribution of allelic and genotyping frequency of A1/A2 allele of beta casein in Badri cattle. Livestock Biodiversity 8 : 115-119.
De Noni I, Cattaneo S (2010) Occurrence of β-casomorphins 5 and 7 in commercial dairy products and in their digests following in vitro simulated gastro-intestinal digestion. Food chemistry 119:560-566
Dinç H (2009) Genotyping of beta-casein, kappa-casein and beta-lactoglobulin genes in turkish native cattle breeds and efforts to delineate BCM-7 on human PBMC, 187.
Eigel W, Butler J, Ernstrom C, Farrell Jr H, Harwalkar V, Jenness R, Whitney RM (1984) Nomenclature of proteins of cow's milk: fifth revision. Journal of dairy science 67 : 1599-1631.
Farrell H, Jimenez-Flores R, Bleck G, Brown E, Butler J, Creamer L, Hicks C, Hollar C, Ng-Kwai-Hang K, Swaisgood H (2004) Nomenclature of the proteins of cows’ milk—Sixth revision. Journal of dairy science 87: 1641-1674.
Firouzamandi, M., Gholami, M., Toloui, M., Eshghi, D. (2018). Genetic Variation of β-Casein Gene Using AS-PCR and ARMS-PCR Techniques in Bovine Populations. Russian Journal of Genetics, 54(11), 1352-1357.
Ganguly I, Gaur G, Singh U, Kumar S, Kumar S, Mann S (2013) Beta-casein (CSN2) polymorphism in Ongole (Indian zebu) and Frieswal (HF× Sahiwal crossbred) cattle. Indian Journal of Biotechnology 12: 195-198.
Groves M (1969) Some minor components of casein and other phosphoproteins in milk. A review. Journal of dairy science 52 : 1155-1165.
Jaiswal KP, Sarsavan A (2013) In silico analysis of Single Nucleotide Polymorphism’s (SNP’s) in Karan Fries cattle of A1, A2 beta casein gene. Int. J Environ. Anim. Conserv 2 : 29-34.
Jawane V, Ali SS, Kuralkar S, Bankar P (2018) Genetic polymorphism of β-casein (CSN2) in Indian Zebu and HF crossbreds. Indian J Dairy Sci 71 : 530-533.
Kamiński S, Cieślińska A, Kostyra E (2007) Polymorphism of bovine beta-casein and its potential effect on human health. Journal of applied genetics. 48 : 189-198.
Lien S, Aleström P, Klungland, H, Rogne S (1992) Detection of multiple β‐casein (CASB) alleles by amplification created restriction sites (ACRS). Animal Genetics. 23: 333-338.
Mapiye C, Chikwanha OC, Chimonyo M, Dzama K, (2019). Strategies for sustainable use of indigenous cattle genetic resources in Southern Africa. Diversity, 11(11), 214.
Martien AM, Groenen J van der Poel, J (1994) Regulation of expression of milk protein genes: a review. Livestock Production Science.38: 61-78.
McLachlan C (2001) β-casein A1, ischaemic heart disease mortality and other illnesses. Medical Hypotheses 56: 262-272.
Mishra B, Mukesh M, Prakash B, Sodhi M, Kapila R, Kishore A, Kataria R, Joshi B, Bhasin V, Rasool T (2009) Status of milk protein, b-casein variants among Indian milch animals. Indian J Anim Sci 79 : 722-725.
Muhammed E, Stephen M (2012) Beta casein A1A2 polymorphism and milk yield in Vechur, Kasargode dwarf and Crossbred cattle. Journal of Indian Veterinary Association Kerala (JIVA) 10: 5-9.
Naik L (2013) Health Implication, Science behind the A1 and A2 Milk. Indian Foodex 27-31.
Navyashree TC (2014) Study on Beta Casein Polymorphism in Malnad Gidda and Crossbred Cattle (Doctoral dissertation).
Nguyen DD, Johnson SK, Busetti F, et al: Formation and degradation of beta-casomorphins in dairy processing. Critical reviews in food science and nutrition 55:1955-1967, 2015.
Niki R, Kim G, Kimura T, Takahashi K, Kohyama K (1994) Physical properties and microstructure of rennet gels from casein micelles of different sizes. Milchwissenschaft 49: 325-329.
Pandey A, Thakur M, Pandey Y (2018) Polymorphism of beta (β) casein gene and their association with milk production traits in Sahiwal and HF crossbred cattle. Indian Journal of Animal Research 53: 847-851.
Priyadarshini P, Mishra C, Mishra B, Swain K, Rout M Mishra, SP (2018) Impact of milk protein on human health: A1 verses A2. Int J Chem Stud 6: 531-535.
Rijnkels M (2002) Multispecies comparison of the casein gene loci and evolution of casein gene family. Journal of mammary gland biology and neoplasia 7: 327-345.
Roginski H, Fuquay, JW,Fox, PF (2003) Encyclopedia of dairy sciences. Volumes 1-4, Academic press, p.
Sebastiani, C., Arcangeli, C., Ciullo, M., Torricelli, M., Cinti, G., Fisichella, S., Biagetti, M. (2020). Frequencies Evaluation of β-Casein Gene Polymorphisms in Dairy Cows Reared in Central Italy. Animals, 10(2), 252.
Shah N, P (2000) Effects of milk-derived bioactives: an overview. British Journal of Nutrition 84: 3-10.
Sharma V, Sharma N, Singh PR, Jawed B, Chandra S (2013) Amplification of the Bovine Beta Casein Gene-Relevance to Modern Human Health. Am. J. PharmTech. Res, 3: 439-444.
Signorelli F, Contarini G, Annicchiarico G, Napolitano F, Orrù L, Catillo G, Haenlein GFW, Moioli B. (2008). Breed differences in sheep milk fatty acid profiles: Opportunities for sustainable use of animal genetic resources. Small Ruminant Research, 78(1-3), 24-31.
Sodhi M, Mukesh M, Mishra B, Kishore A, Prakash B, Kapil R, Khate K, Kataria R,Joshi B (2012) Screening of taurine and crossbred breeding bulls for A1/A2 variants of β-casein gene. The Indian Journal of Animal Sciences 82: 2-9.
Sodhi M, Kataria R, Niranjan S, Parvesh K, Verma P, Swami S, Sharma A, Bharti V, Kumar B,Iqbal M (2018) Sequence characterisation and genotyping of allelic variants of beta casein gene establishes native cattle of Ladakh to be a natural resource for A2 milk. Def. Life Sci. J 3: 177-181.
Srinivas B, Aswani K. K Padmaja, K, Punyakumari B (2019) Genotyping and protein profiling of milk β-casein variants (A1 and A2) in of deoni, sahiwal and malnad gidda breeds of milch cattle. The Pharma Innovation Journal 8: 181-186.
Srivastava AK, Patel JB, Ankuya KJ, Chauhan HD, Pawar MM, Gupta JP. (2019). Conservation of indigenous cattle breeds. Journal of Animal Research, 9(1), 1-12.
Sun Z, Cade JR, Fregly MJ, Privette RM (1999) β-Casomorphin induces Fos-like immunoreactivity in discrete brain regions relevant to schizophrenia and autism. Autism 3: 67-83.
Şahin Ö, Boztepe S, Aytekin İ (2018) A1 and A2 Bovine Milk, the Risk of Beta-casomorphin-7 and Its Possible Effects on Human Health:(II) Possible Effects of Beta-casomorphin-7 on Human Health. Selcuk Journal of Agriculture and Food Sciences 32:640-645
Woodford K (2007) A2 milk, farmer decisions, and risk management. Proceedings of the 16th International Farm Management Congress: Peer reviewed papers. 641-648.
Yeh FC, Rongcai Y, Boyle, T, Ye Z, Xiyan JM (1997) POPGENE, the Userfriendly Shareware for Population Genetic Analysis. Edmonton, Alberta, Canada. Molecular Biology and Biotechnology Centre.
Zepeda JL, Alarcón-Zúñiga B, Ruíz-Flores A, Núñez-Domínguez R, Ramírez-Valverde R (2015) Polymorphism of three milk protein genes in Mexican Jersey cattle. Electronic Journal of Biotechnology 18: 1-4.