The influence of phytogenic additive on the antioxidant capacity, immunity and liver functions in stress-induced male rats

Published: Νοε 9, 2022
Updated: 2022-11-09
2022-11-09 (3)
Antioxidant status feed additives immunity phytogenic ingredients stress.
E Koseli
N Seyidoglu
R Gurbanli
C Aydin

Global animal feeding strategies have been modified due to several ecosystem changes that cause stress and decline in health, growth, and yields. According to the literature, natural additives have been fundamental to animal health from day to day. The present study measured changes in antioxidant status, immunity, liver functions and organ weights tested under stress conditions to determine whether dietary supplementation with phytogenic additive could provide beneficial effects. Forty-eight adult, male Sprague-Dawley rats were randomly separated into four groups; Control(C), Stress(S), Treatment(Tr), Treatment and Stress(TrS). Rats in groups Tr and TrS received phytogenic additive by adding into water (2mL/L) 5days a week for 28days. All rats were exposed to prolonged light phase conditions (18h light: 6h dark) for 14 days. Also, two chronic stresses, isolation and crowded environments, were applied to animals in the Stress and TrS groups. There was a significant decline in the oxidant status in untreated stress group, while phytogenic additive fed rats maintained a significantly higher total antioxidant status. This study also showed a significant increase in IL-4 and decrease in IFN-γ in the untreated Stress group compared to the Control group. There were increases in liver enzymes in the Stress group in comparison to the Control group. After the phytogenic treatment, there was an increase in the weight of the liver, intestine, brain and testes. In conclusion, this study showed that supplementation of phytogenic additive containing milk thistle and artichoke with choline, carnitine, vitamin E and melatonin describes the protective effects on antioxidant status, immunological parameters and liver functions under mixed stress conditions.

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Ahmed SF, Abd Al Haleem EN, El-Tantawy WH (2019) Evaluation of the anti-atherogenic potential of Egyptian artichoke leaf extract in hypercholesterolemic rats. Arch Physiol Biochem 28: 1-12.
Aydin C, Seyidoglu N (2021) Natural Antioxidants to the Rescue? In: Antioxidants - Benefits, Sources, Mechanisms of Action. IntechOpen, England (online first).
Ahmad M, Chand N, Khan RU, Ahmad N, Khattak I, Naz S (2020) Dietary supplementation of milk thistle (Silybum marianum): growth performance, oxidative stress, and immune response in natural summer stressed broilers. Trop Anim Health Prod 52; 711-715.
Baurhoo B, Phillip L, Ruiz-Feria CA (2007) Effects of purified lignin and mannan oligosaccharides on intestinal integrity and microbial populations in the ceca and litter of broiler chickens. Poult Sci 86: 1070–1078.
Chuian OM, Temur’iants NA, Makhonina MM, Zaiachnikova TV (2005) Effect of hypokinetic stress and low intensity electromagnetic field of extremely high frequency of changes of cytokine concentration in rat blood. Fiziol Zh 51: 70-78.
Cichoz-Lachi H, Michalak A (2014) Oxidative stress as a crucial factor in liver diseases. World J Gastroenterol 20: 8082–8091.
Colditz IG, Hine BC (2016) Resilience in farm animals: biology, management, breeding and implications for animal welfare. Anim Prod Sci 56: 1961-1983.
Contreras-Zentella ML, Hernández-Muñoz R (2016) Is liver enzyme release really associated with cell necrosis induced by oxidant stress? Oxid Med Cell Longev 12: 1-12.
Curtin NM, Boyle NT, Mills KH, Connor TJ (2009) Psychological stress suppresses innate IFN-gamma production via glucocorticoid receptor activation: reversal by the anxiolytic chlordiazepoxide. Brain Behav Immun 23: 535-47.
Dumari MA, Sarir H, Makki OF, Afzali N (2014) Effect of Milk Thistle (Silybum marianum L.) on Biochemical Parameters and ımmunity of broiler chicks fed aflatoxin B1 after three weeks. Iranian J Toxicol 8(26):1098-1103.
Erel O (2005) A new automated colorimetric method for measuring total oxidant status. Clinical Biochem 38: 1103-1111.
Gancarczyk M, Paziewska-Hejmej A, Carreau S, Tabarowski Z, Bilińska B (2004) Dose- and photoperiod- dependent effects of 17beta-estradiol and the anti-estrogen ICI 182,780 on testicular structure, acceleration of spermatogenesis, and aromatase immunoexpression in immature bank voles. Acta Histochem 106(4): 269-278.
Glaser R, Kiecolt-Glaser JK (2005) Stress-induced immune dysfunction: implications for health. Nat Rev Immunol 5(3): 243–251.
Gomes AVS, Quinterio-Filho WM, Ribeiro A, Ferraz-de-Paula V, Pinheiro ML, Baskeville E, Akamine AT, Astolfi-Ferrira CS, Ferrira AJP, Palermo-Neto J (2014) Overcrowding stress decreases macrophage activity and increases Salmonella Enteritidis invasion in broiler chickens. Avian Pathol 43(1): 82-90.
Gostin AI, Waisundara VY (2019) Review. Edible flowers as functional food: A review on artichoke (Cynara cardunculus L.). Trends Food Sci Technol 86: 381-391.
Guardia S, Konsak B, Combes S, Levenez F, Cauquil L, Guillot JF, Moreau-Vauzelle C, Lessire M, Juin H, Gabriel I (2011) Effects of stocking density on the growth performance and digestive microbiota of broiler chickens. Poult Sci 90: 1878-1889.
Gulati K, Guhathakurta S, Joshi J, Rai N, Ray A (2016) Cytokines and their Role in Health and Disease: A Brief Overview. MOJ Immunol 4(2): 00121.
Hong JC, Steiner T, Aufy A, Lien TF (2012) Effects of supplemental essential oil on growth performance, lipid metabolites and immunity, intestinal characteristics, microbiota and carcass traits in broilers. Livest Sci 144: 253-262.
Houshmand M, Azhar K, Zulkifli I, Bejo MH, Kamyab A (2012) Effects of prebiotic, protein level, and stocking density on performance, immunity, and stress indicators of broilers. Poult Sci 91: 393-401.
Johnson RW, Escobar J (2005) Cytokine regulation of protein accretion in growing animals. In: Biology of metabolism in growing animals. Elsevier, Edinburgh: pp 83-106.
Koseli E, Seyidoglu N, Aydin C (2019) Effects of phytogenic ingredients on haematological and morphological parameters in stress exposed male rats. Bulgarian Journal of Veterinary Medicine 22 Suppl.1: 40-49.
Koseli E, Seyidoglu N, Karakci D, Gurbanli R, Aydin C (2021) Effects of essential oils as natural additives on serum irisin, hepcidin and protein levels in a rat model of mixed stress. In: Proceedings of the 6th Internatıonal Conference On Agriculture, Animal Husbandry and Rural Development, Siirt, Turkey: pp 321-333.
Kus I, Songur A, Ozogul C, Kavakli A, Zararsiz I, Sarsılmaz M (2004) Effects of photoperiod on the ultrastructure of leydig cells in rat. Arch of Androl 50: 193-200.
Lee DY, Kim E, Choi MH (2015) Technical and clinical aspects of cortisol as a biochemical marker of chronic stress. BMB Rep 48(4): 209-216.
Li W, Wei F, Xu B, Sun Q, Deng W, Ma H, Bai J, Li S (2019) Effect of stocking density and alpha-lipoic acid on the growth performance, physiological and oxidative stress and immune response of broilers. Asian-Australas J Anim Sci 32(12): 1914-1922.
Mahjoor AA, Denghan A (2008) Effect of Silymarin on metabolic factors of food-restricted over conditioned Wistar rats. Pak J Biol Sci 11(14): 1835-1839.
Marin MT, Cruz FC, Planeta CS (2007) Chronic restraint, or variable stresses differently affect the behavior, corticosterone secretion and body weight in rats. Physiol Behav 90(1): 29‐35.
Meddings JB, Swain MG (2000) Environmental stress-induced gastrointestinal permeability is mediated by endogenous glucocorticoids in the rat. Gastroenterol 119: 1019-1028.
Ménard C, Pfau M, Hodes G, Russo SJ (2017) Immune and Neuroendocrine Mechanisms of Stress Vulnerability and Resilience. Neuropsychopharmacol 42: 62-80.
Mering S, Kaliste-Korhonen E, Nevalainen T (2001) Estimates of appropriate number of rats: interaction with housing environment. Lab Anim 35(1): 80-90.
Mormède C, Castanon N, Médina C, Moze E, Lestage J, Neveu PJ, Dantzer R (2002) Chronic mild stress in mice decreases peripheral cytokine and increases central cytokine expression independently of IL-10 regulation of the cytokine network. Neuroimmun 10(6): 359-366.
Murakami M, Sato N, Sato N, Nakamura T, Masunaga H. (2007) Changes in lymphocyte phenotypes and cytokine production by surgical stress in a rat small intestinal resection model. J Clin Biochem Nutr 40: 216-20.
Picard M, McEwen S (2018). Psychological Stress and Mitochondria: A Systematic Review. Psychosom Med 80(2): 141-153.
Qawami N, Badi HN, Labbafi MR, Mehrafarin A (2013) A Review on Pharmacological, Cultivation and Biotechnology Aspects of Milk Thistle (Silybum marianum (L.) Gaertn.). J Med Plant 12(47): 19-37.
Quezada-Mendoza VC, Heinrichs AJ, Jones CM (2011) The effects of a prebiotic supplement (Prebio Support) on fecal and salivary iga in neonatal dairy calves. Livest Sci 142: 222-228.
Rohleder N, Aringer M, Boentert M (2012) Role of interleukin-6 in stress, sleep, and fatigue. Ann N Y Acad Sci 1261: 88-96.
Samarghandian S, Afshari R, Farkhondeh T (2014). Effect of long-term treatment of morphine on enzymes, oxidative stress indices and antioxidant status in male rat liver. Int J Clin Exp Med 7: 1449-1453.
Sanchez-Valle V, Chavez-Tapia NC, Uribe M, Méndez-Sánchez N (2012) Role of oxidative stress and molecular changes in liver fibrosis: A review. Curr Med Chem 19: 4850-4860.
Saymore PN, Muchenje V, Chimonyo M (2011) Animal welfare in multipurpose cattle production systems and its implications on beef quality. Afr J Biotechnol 10(7): 1049-1064.
Sejian V, Maurya VP, Naqvi SMK (2010) Effect of thermal stress, restricted feeding and combined stresses (thermal stress and restricted feeding) on growth and plasma reproductive hormone levels of Malpura ewes under semiarid tropical environment. J Anim Physiol Anim Nutr 95(2): 252-258.
Seyidoglu N, Koseli E, Aydin C (2019a) Effects of several essential oils on haematological and morphological parameters in stress exposed male rats. Bulg J Vet Med 22(Suppl. 1): 50-58.
Seyidoglu N, Gurbanli R, Koseli E, Cengiz F, Aydin C (2019b) The effects of Spirulina (Arthrospira) platensis on morphological and hematological parameters evoked by social stress in male rats. JIVS 3(1): 21-27.
Seyidoglu N, Aydin C (2020) Stress, Natural Antioxidants and Future Perspectives. In: The Health Benefits of Foods - Current Knowledge and Further Development. Intechopen press, London: pp 149-165.
Seyidoğlu N, Köşeli E, Gurbanlı R, Aydın C (2021) The preventive role of Spirulina platensis (Arthrospira platensis) in immune and oxidative insults in a stress-induced rat model. J Vet Res 65: 193-200.
Seyidoglu N, Koseli E, Karakci D, Gurbanli R, Aydin C (2021) Effects of phytogenic feed supplementation on serum irisin, hepcidin and protein levels ın a rat model of mixed stress. In: Proceedings of the 5th International Erciyes Conference On Scientific Research, Kayseri, Turkey: pp 123-130.
Takahashi A, Flanigan ME, McEwen BS, Russo SJ (2018) Aggression, Social Stress, and the Immune System in Humans and Animal Models. Front Behav Neurosci 12: 56.
Tao L, Zhong S, Liao X, Chen J, He T, Lai S, Jia Y (2015) A meta-analysis of oxidative stress markers in depression. PLoS One 10(10): e0138904.
Ten Hoor F, Rietveld WJ, Kooij M, Flory W (1980) Growth and 24 hour eating patterns of rats kept under various light: dark conditions. Lab Anim 14: 251.
Wilasrusmee C, Kittur S, Shah G, Siddiqui J, Bruch D, Wilasrusmee S, Kittur DS (2002) Immunostimulatory effect of Silybum marianum (Milk Thistle) extract. Med Sci Monit 8: 439-443.
Yildiz A, Hayirli A, Okumus Z, Kaynar O, Kisa F (2007). Physiological profile of juvenile rats: Effects of cage size and cage density Lab Anim 36: 28-38.
Yang PC, Jury J, Soderholm JD, Sherman PM, McKay DM, Perdue MH (2006) Chronic psychological stress in rats induces intestinal sensitization to luminal antigens. Am J Pathol 168: 104-14.
Zlatkovic J, Todorovic N, Tomanovic N, Bošković M, Djordjević S, Lazarević-Pašti T, Bernardi RE, Djurdjević A, Filipović D (2014). Chronic administration of fluoxetine or clozapine induces oxidative stress in rat liver: A histopathological study. Eur J Pharm Sci 59: 20-30.
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