Expression profiling of IL-1β, IL-6 and IL-8 genes in lung tissues of Aseel, Crossbred Naked neck, and White Leghorn chicken challenged with H9N2 Influenza virus

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Published: Jan 21, 2023
DOI: https://doi.org/10.12681/jhvms.28174
Keywords:
Interleukin genes expression Avian influenza virus (H9N2) Aseel Naked Neck White Leghorn
KM Malik
Department of Animal Breeding and Genetics, University of Veterinary and Animal Sciences, Lahore, Pakistan
https://orcid.org/0000-0001-7497-368X
I Zahoor
Department of Animal Breeding and Genetics, University of Veterinary and Animal Sciences, Lahore, Pakistan
https://orcid.org/0000-0002-5960-025X
A Khalique
Department of Animal Nutrition, University of Veterinary and Animal Sciences, Lahore, Pakistan
https://orcid.org/0000-0001-7768-3880
AW Sahota
Department of Poultry Production, University of Veterinary and Animal Sciences, Lahore, Pakistan
https://orcid.org/0000-0002-9765-7310
A Basheer
Department of Animal Breeding and Genetics, University of Veterinary and Animal Sciences, Lahore, Pakistan
Abstract

Aseel and Naked neck are major chicken breeds of the tropics and well-known for their thermotolerance and robustness. However, both of them especially Aseel are very susceptible to Avian Influenza (AI) which cause huge mortalities. The role of cytokines in relation to the pathology and severity of the disease caused by the endemic strain (H9N2) of AIV in these breeds remained thus far unclear. The aim of this study was to investigate the effects of H9N2 AIV on the expression level of IL-1β, IL-6 and IL-8 in the lung tissues of Aseel, crossbred Naked Neck, and White Leghorn (WLH). In total 60 birds, 20 from each breed, were used in this study, whereas 30 birds (10 from each breed) were challenged intranasally with H9N2 virus with a concentration of 106 EID50 at 6wk of age and other half were treated as control. The lung tissues were sampled at 5th day post-infection to study the differential expression of IL-1β, IL-6, and IL-8 using qRT-PCR. Our data revealed a significant difference (P<0.001) in the gene expression levels among all the breeds in response to viral challenge. It was also observed that after exposure to H9N2 virus, Aseel birds showed the highest increase in their expressions of interleukin (IL-1 β, IL-6, and IL-8) genes followed by Naked Neck and WLH respectively suggesting greater susceptibility of Aseel to AIV compared with other breeds. However, these results are in agreement with the severity of disease and incidence of mortality caused by AI in these breeds.

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