Fosfomycin Resistant Enterobacterales IsolatedFrom Chicken Meat in Turkey

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Published: Apr 18, 2023
Updated: 2023-04-18
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2023-04-18 (2)
DOI: https://doi.org/10.12681/jhvms.28245
Keywords:
Fosfomycin resistance chicken meat fosA3 gene fosA4 gene Turkey
S Al
Erciyes University
https://orcid.org/0000-0001-8255-3802
HB Dişli
Hatay Mustafa Kemal University
C Kurekci
Hatay Mustafa Kemal University
https://orcid.org/0000-0002-6442-2865
Abstract

This study was conducted to obtain the relative prevalence of fosfomycin resistant (FOSr) Enterobacterales in raw chicken meat samples in Turkey. Samples (n=85) were enriched in non-selective media and transferred to MacConkey agar plates containing FOS and glucose-6-phosphate. As a result, FOSr Enterobacterales isolates were detected by a selective method in 27% of raw chicken meat samples (n=23) and identified as Escherichia coli (21/26), Klebsiella oxytoca (2/26), Escherichia vulneris (1/26), Raoultella terrigena (1/26) and Kluyvera intermedia (1/26). PFGE analysis showed 16 different band patterns in Escherichia spp. isolates (n=22) based on the 85% similarity. The minimum inhibitory concentration for FOS against all isolates was determined to be ≥64 mg/L. In addition, the highest rate of resistance was determined for nalidixic acid (72.7%), ampicillin (68.2%), tetracycline (59.1%), trimethoprim-sulfamethoxazole (54.5%), and chloramphenicol (59.1%) among all Escherichia isolates. PCR screening and sequencing identified the presence of fosA4 and fosA3 genes in ten (47.6%) and seven (33.3%) E. coli isolates, respectively. The fosA3 gene has also appeared in K. intermedia, and R. terrigena isolates.  Only two E. coli isolates were positive for the blaCTX-M-55 gene, whereas the aac(6')-Ib-cr gene was identified in eight E. coli and one K. intermedia isolates. In addition, 19 different replicon types were determined by PCR-based plasmid replicon typing with IncFII (n=20) being the most common and followed by IncI1α (n=10), IncFIIS (n=8), and IncFIB (n=8). We report, to our knowledge, the first evidence on the presence of FOSr Enterobacterales isolates in raw chicken meat samples in Turkey that might be an important reservoir for FOSr organisms to humans.

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